A5090704182- Advanced NMR Techniques and Applications
- Protein Structure and Dynamics
- Molecular spectroscopy and chirality
- Solid-state spectroscopy and crystallography
- NMR spectroscopy and applications
- Crystallography and Radiation Phenomena
- Enzyme Structure and Function
- Antimicrobial Resistance in Staphylococcus
- Muon and positron interactions and applications
- Spectroscopy and Quantum Chemical Studies
- RNA and protein synthesis mechanisms
- Antimicrobial Peptides and Activities
- RNA modifications and cancer
- Probiotics and Fermented Foods
- Microbial Metabolism and Applications
- RNA Research and Splicing
- Lipid Membrane Structure and Behavior
- Antibiotic Resistance in Bacteria
Institute of Science and Technology Austria
2022-2023
Utrecht University
2022
Goethe University Frankfurt
2022
Abstract Antibiotics that use novel mechanisms are needed to combat antimicrobial resistance 1–3 . Teixobactin 4 represents a new class of antibiotics with unique chemical scaffold and lack detectable resistance. targets lipid II, precursor peptidoglycan 5 Here we unravel the mechanism teixobactin at atomic level using combination solid-state NMR, microscopy, in vivo assays molecular dynamics simulations. The enduracididine C-terminal headgroup specifically binds pyrophosphate-sugar moiety...
Abstract Aromatic side chains are important reporters of the plasticity proteins, and often form contacts in protein–protein interactions. We studied aromatic residues two structurally homologous cross‐β amyloid fibrils HET‐s, HELLF by employing a specific isotope‐labeling approach magic‐angle‐spinning NMR. The dynamic behavior Phe Tyr indicates that hydrophobic core is rigid, without any sign “breathing motions” over hundreds milliseconds at least. exposed fibril surface have rigid ring...
Abstract Control of posttranscriptional mRNA decay is a crucial determinant cell homeostasis and differentiation. lifetime governed by cis-regulatory elements in their 3′ untranslated regions (UTR). Despite ongoing progress the identification cis we have little knowledge about functional structural integration multiple 3′UTR regulatory hubs recognition mRNA-binding proteins (RBPs). Structural analyses are complicated inconsistent mapping prediction RNA fold, dynamics, size. We here, for...
Abstract. Dynamic processes in molecules can occur on a wide range of timescales, and it is important to understand which timescales motion contribute different parameters used dynamics measurements. For spin relaxation, this easily be understood from the sampling frequencies spectral-density function by relaxation-rate constants. In addition data relaxation measurements, determining dynamically averaged anisotropic interactions magic-angle spinning (MAS) solid-state NMR allows for better...
Probing the dynamics of aromatic side chains provides important insights into behavior a protein because flips rings in protein's hydrophobic core report on breathing motion involving large part protein. Inherently invisible to crystallography, motions have been primarily studied by solution NMR. The question how packing proteins crystals affects ring has, thus, remained largely unexplored. Here we apply magic-angle spinning NMR, advanced phenylalanine
Probing the dynamics of aromatic side chains provides important insights into behavior a protein because flips rings in protein’s hydrophobic core report on breathing motion involving large part protein. Inherently invisible to crystallography, motions have been primarily studied by solution NMR. The question how packing proteins crystals affects ring has, thus, remained largely unexplored. Here we apply magic-angle spinning NMR, advanced phenylalanine 1 H- 13 C/ 2 H isotope labeling and MD...
Abstract. Dynamic processes in molecules can occur on a large range of time scales, and it is important to understand which scales motion contribute different parameters used dynamics measurements. For spin relaxation, this easily be understood from the sampling spectral-density function by relaxation-rate constants. In addition data relaxation measurements, determining dynamically-averaged anisotropic interactions magic-angle spinning (MAS) solid-state NMR allows better quantification...
Table S1.Summary of simulation parameters.In simulations heteronuclear two-spin systems (CP, REDOR, wPARS and off-MAS) I spins correspond to protons S 13 C.More than 538 powder orientations are only necessary for quadrupolar spectra.All programs freely available in the data repository.
Rock-solid amyloid?. Aromatic residues in the core of amyloid fibrils HELLF and HET-s show no sign ring flips suggesting a rigid that does not undergo "breathing motions". facing outside on other hand are highly flexible various timescales. These conclusions could be drawn from dynamics measurements by solid-state MAS NMR combination with specific labeling phenylalanines tyrosines as Paul Schanda et al. describe their Research Article (e202219314). Coordination Compounds Nanocatalysis...
Abstract Aromatische Seitenketten sind wichtige Indikatoren für die Plastizität von Proteinen und bilden oft entscheidende Kontakte bei Protein‐Protein‐Wechselwirkungen. Wir untersuchten aromatische Reste in den beiden strukturell homologen cross‐β Amyloidfibrillen HET‐s HELLF mit Hilfe eines spezifischen Ansatzes zur Isotopenmarkierung Festkörper NMR Drehung am magischen Winkel. Das dynamische Verhalten der aromatischen Phe Tyr deutet darauf hin, dass hydrophobe Amyloidkern starr ist keine...