How to find early gastric cancer cells in vivo is a great challenge for the diagnosis and therapy of cancer. This study aimed at investigating feasibility using fluorescent magnetic nanoparticle (FMNP)-labeled mesenchymal stem (MSCs) realize targeted imaging hyperthermia The primary cultured mouse marrow MSCs were labeled with amino-modified FMNPs then intravenously injected into model subcutaneous tumor, then, distribution FMNP-labeled was observed by fluorescence system resonance system....

Abstract Atopic dermatitis (AD) is a common chronic pruritic inflammatory skin disorder characterized by recurrent eczematous lesions. Interleukin (IL)−33, cytokine of the IL-1 family, was found to play an important role in pathogenesis AD. As key component inflammasome, NLRP3 has been mostly described myeloid cells that mediate inflammasome activation conducted proinflammatory production family. However, AD, as well IL-33 processing are highly controversial. Whether can expression and...

Abstract Manganese (Mn) overexposure produces long-term cognitive deficits and reduces brain-derived neurotrophic factor (BDNF) in the hippocampus. However, it remains elusive whether Mn-dependent enhanced alpha-synuclein (α-Syn) expression, suggesting a multifaceted mode of neuronal toxicities, accounts for interference with BDNF/TrkB signaling. In this study, we used C57BL/6J WT α-Syn knockout (KO) mice to establish model manganism found that Mn-induced impairments spatial memory synaptic...

An injectable and thermo-sensitive hydrogel was derived from AM, which can be injected solidified in vivo . The dAM-gel offers an option for preventing IUA by promotion of the re-epithelialization damaged endometrium.

Liposomal honokiol isolated from the genus Magnolia has been found to have antiangiogenic, anti-inflammatory and antitumor properties. However, there no report on its role in hair growth. Hair follicles are life-long cycled organelles that go through anagen, catagen telogen stages regulated by diverse signaling pathways, including Wnt/β-catenin, Notch, Epidermal growth factor (EGF) Sonic hegehog (SHH). Wnt signals essential for initiation of follicle placode development a new potential...

Decellularized extracellular matrix (dECM) is a valuable tool for generating three-dimensional in vitro tumor models that effectively recapitulate tumor-extracellular (ECM) interactions. However, current culture models, the components and structures of dECM are enzymatically disrupted to form hydrogels, making it difficult native ECM. Additionally, when studying ECM-cell interactions, large-volume incompatible with traditional experimental techniques nutrient-oxygen concentration gradient,...

Glioblastoma (GBM), the most common primary malignant brain tumor, has a poor prognosis, with median survival of only 14.6 months. The Warburg effect is an abnormal energy metabolism, which main cause acidic tumor microenvironment. This study explored role in prognosis and immune microenvironment GBM.A prognostic risk score model effect-related genes (Warburg signature) was constructed using GBM cohort data from Cancer Genome Atlas. Cox analysis performed to identify independent factors....

Abstract Background Skull base chordoma is a rare and aggressive tumour of the bone that has high likelihood recurrence. The fundamental differences in single cells between primary recurrent lesions remain poorly understood, impeding development effective treatment approaches. Methods To obtain an understanding chordomas, we performed single‐cell RNA sequencing T‐cell/B‐cell receptor (BCR) sequencing. This allowed us to delineate two types cells, tumour‐infiltrating lymphocytes, myeloid...

Abstract Overexposure to manganese (Mn) has been known induce alpha‐synuclein (α‐Syn) oligomerization, which is degraded mainly depending on endoplasmic reticulum stress (ER stress) and autophagy pathways. However, little data reported the cross‐talk between ER Mn‐induced α‐Syn oligomerization. To explore relationship autophagy, we used 4‐phenylbutyric acid (4‐PBA, inhibitor), rapamycin (Rap, activator) 3‐methyladenine (3‐MA, inhibitor) in mice model of manganism. After 4 weeks treatment...

Enhancer release and retargeting (ERR) events could activate disease-causing gene promoters for increasing the expression level of oncogenes. Meanwhile, class A orphan GPCRs (oGPCRs) are known as potential biomarkers or drug targets various cancers, such gastric cancer (GC). Hence, systemic investigation ERR oGPCRs in GC help to explore GC. In this study, ENCODE GTEx eQTL data were utilized define Only GPR35 was then detected that be activated by based on these ChIP-seq. Then, functional...

We conducted a multicenter clinical study to construct novel index based on combination of albumin-globulin score and sarcopenia (CAS) that can comprehensively reflect patients' nutritional inflammatory status assess the prognostic value CAS in renal cell carcinoma (RCC) patients. Between 2014 2019, 443 patients from 3 centers who underwent nephrectomy were collected (343 training set 100 test set). Kaplan-Meier curves employed analyze impact ratio (AGR), (AGS), sarcopenia, overall survival...

Gliomas, the most common primary tumors in central nervous system (CNS), can be categorized into 4 grades according to World Health Organization. The malignant glioma type is grade Ⅳ, also named glioblastoma multiforme (GBM). However, standard treatment of concurrent temozolomide (TMZ) chemotherapy and radiotherapy after maximum resection does not improve overall survival patients with GBM. Targeting components CNS microenvironment represents a new strategy for improving efficacy treatment....

We sought to compare the symptomatic radiation pneumonitis (RP) in lung cancer patients treated with helical tomotherapy (HT) versus intensity-modulated radiotherapy (IMRT), and examine predictive value of circulating lymphocyte subsets affecting occurrence RP.Circulating subsets, clinical characteristics, dosimetric parameters pulmonary function were collected from 130 HT (n = 53) or IMRT 77) 2016 through 2020. Symptomatic RP was compared between groups. Binary logistic regression used...

Tumor-infiltrating T-lymphocytes are defined as that infiltrated into tumor tissues; however, their composition, clinical significance, and underlying mechanism in hepatocellular carcinoma (HCC) adjacent non-tumor tissues still not completely understood. Herein, we collected marker genes of T cell subpopulations from a previous study estimated relative infiltrating levels HCC tissues. Specifically, the all cells were significantly reduced compared with Unsupervised clustering samples by...

Medulloblastoma, a common pediatric malignant tumor, has been recognized to have four molecular subgroups [wingless (WNT), sonic hedgehog (SHH), group 3, 4], which are defined by the characteristic gene transcriptomic and DNA methylomic profiles, distinct clinical features within each subgroup. The tumor immune microenvironment is integral in initiation progression might be associated with therapeutic responses. However, date, infiltrative landscape of medulloblastoma not yet elucidated....

612 Background: Pancreatic Adenocarcinoma (PA) is a leading cause of cancer-related death across the world, with poorer outcomes noted in minority populations. The observed variation has been attributed to delayed diagnosis, barriers optimal treatment and health care related disparities. We evaluated impact race on clinical presentation pancreatic cancer at large academic teaching center. Methods: performed retrospective review patients adenocarcinoma diagnosed between January 2016 December...

Abstract Pyrogen, often as a contaminant, is key indicator affecting the safety of almost all parenteral drugs (including biologicals, chemicals, traditional Chinese medicines and medical devices). It has become goal to completely replace in vivo rabbit pyrogen test by using vitro based on promoted ‘reduction, replacement refinement’ principle, which been highly considered regulatory agencies from different countries. We used NF-κB, central signalling molecule mediating inflammatory...

Background: The abnormal expression of α-arrestin protein family plays a key regulatory role in the occurrence and development many cancers, including colorectal cancer cervical cancer, is inseparable from changes tumor immune microenvironment. However, ARRDC2, an important member this family, malignant biological process ovarian (OC) has not been reported, its change microenvironment also unknown. Methods: In study, HPA, TCGA, GEO other databases were used to explore ARRDC2 prognosis...

e18657 Background: Pancreatic Adenocarcinoma (PA) is a leading cause of cancer-related death across the world, with poorer outcomes observed in minority populations. The variation has been attributed to delayed diagnosis, barriers optimal treatment and health care related disparities. We sought evaluate impact race on clinical presentation pancreatic cancer at large academic teaching center. Methods: performed retrospective review patients adenocarcinoma diagnosed between January 2016...

Objective To assess the safety and feasibility of robotic-assisted multivisceral resection for advanced colorectal cancer. Methods From Nov. 2014 to Dec. 2017, patients who underwent cancer were investigated. Patient demographics, treatment characteristics, perioperative outcomes, pathological results evaluated retrospectively. Results There 13 patients; 7 men 6 women, with a median age 60 years. 5 had rectal cancer, 8 sigmoid cT4b tumor, complete primary tumor. The resected...

Abstract Background: Uterine Corpus Endometrial Carcinoma (UCEC) ranks fourth among female cancers in the world. Frustratingly, 5-year survival rates for advanced patients are only 17%. KDM4B is overexpressed or dysregulated a variety of and could be associated with tumor progression poor prognosis. Therefore, we performed bioinformatics analysis vitro assays to assess role UCEC. In addition, its relevance immune cells microenvironment was explored. Methods: The mRNA level protein UCEC...