A5033733622- SARS-CoV-2 detection and testing
- Nosocomial Infections in ICU
- Clostridium difficile and Clostridium perfringens research
- Neonatal Respiratory Health Research
- SARS-CoV-2 and COVID-19 Research
- Advanced biosensing and bioanalysis techniques
- COVID-19 Clinical Research Studies
- COVID-19 Impact on Reproduction
- Antibiotic Use and Resistance
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Enhanced Recovery After Surgery
- Streptococcal Infections and Treatments
- Neonatal Health and Biochemistry
- Palliative Care and End-of-Life Issues
- Cardiac, Anesthesia and Surgical Outcomes
- Respiratory viral infections research
- Inflammatory mediators and NSAID effects
- Antibiotics Pharmacokinetics and Efficacy
- Neutropenia and Cancer Infections
- Hemoglobinopathies and Related Disorders
- Pneumonia and Respiratory Infections
- Legionella and Acanthamoeba research
Boston Children's Hospital
2020-2025
Boston Children's Museum
2021-2025
Harvard University
2020-2023
Boston University
2022
Diagnosis of COVID-19 by PCR offers high sensitivity, but the utility detecting samples with cycle threshold (CT ) values remains controversial. Currently available rapid diagnostic tests (RDTs) for SARS-CoV-2 nucleocapsid antigens (Ag) have sensitivity well below PCR. The correlation Ag and RNA quantities in clinical nasopharyngeal (NP) is unknown. An ultrasensitive, quantitative electrochemiluminescence immunoassay (the MSD S-PLEX N assay) was used to measure NP from adults children...
The distribution of upper respiratory viral loads (VL) in asymptomatic children infected with severe acute syndrome coronavirus 2 (SARS-CoV-2) is unknown. We assessed PCR cycle threshold (Ct) values and estimated VL diagnosed nine pediatric hospital testing programs. Records for symptomatic patients positive clinical SARS-CoV-2 tests were reviewed. Ct (i) adjusted by centering each value around the institutional median from tested that assay (ii) converted to (numbers copies per milliliter)...
Most severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections in pediatric patients are mild or asymptomatic. However, infants have emerged at higher risk of hospitalization and outcomes disease 2019 (COVID-19). We report a case series 4 full-term neonates hospitalized with fever found to SARS-CoV-2 infection spectrum illness severities. Two required admission the intensive care unit for insufficiency end organ involvement. Half were coinfection. One neonate received antiviral...
Abstract Background Clostridioides difficile (C. difficile) infection (CDI) is the most common healthcare-associated in US. Use of molecular diagnostics may be contributing to increasing incidence due high sensitivity these testing modalities, leading over-diagnosis persons with asymptomatic colonization. Colonization C. early infancy. Laxative use cause diarrhea and confound CDI diagnosis. Misdiagnosis can lead over-treatment, increased hospital costs, prolonged stay, misrepresentation true...
Abstract Background Diagnosis of COVID-19 by PCR offers high sensitivity, but the utility detecting samples with cycle threshold (Ct) values remains controversial. Currently available rapid diagnostic tests (RDTs) for SARS-CoV-2 nucleocapsid antigens (Ag) have sensitivity well below PCR. The correlation Ag and RNA quantities in clinical nasopharyngeal (NP) is unknown. Methods An ultrasensitive, quantitative electrochemiluminescence immunoassay (the MSD ® S-PLEX CoV-2 N assay) was used to...
Abstract Purpose Acute bacterial sinusitis is among the most frequent outpatient infections in children and adolescents well suited to study large healthcare utilization databases, but validity of International Classification Diseases, 10th Revision (ICD‐10) codes together with antibiotic prescriptions identify cases acute has not been established. We aimed evaluate ICD‐10 combined new diagnoses pediatric patients evaluated setting. Methods Children aged 17 years younger an diagnosis along...
Abstract Background Gram-negative bacteremia historically has been treated with 10-14 days of parenteral antibiotics. However, data supporting this practice are lacking, and recent evidence shows equivalent outcomes for short-course (SC) therapy (7 days) early (by day 5) conversion to highly bioavailable enteral (PO) antibiotics Enterobacterales bacteremia. Methods Under a QI framework, we used PDSA cycles reduce treatment duration increase use PO levofloxacin or...