The order Piroplasmida (e.g., Babesia, Theileria, Cytauxzoon, and Rangelia) suborder Adelorina Hepatozoon) comprise protozoa of the phylum Apicomplexa, which are related to significant economic, veterinary, medical concerns. This study aimed investigate molecular occurrence evaluate phylogenetic placement Hepatozoon agents in small mammals (rodents marsupials) midwestern Brazil. maximum likelihood inference positioned 18S ribosomal RNA (18S rRNA) sequences obtained from Hylaeamys...

Toxoplasma gondii, Neospora caninum and Sarcocystis neurona are related apicomplexan parasites that cause reproductive neurological disorders in a wide range of domestic wild animals. In the present study, immunofluorescence antibody test (IFAT) was used to investigate presence antibodies against T. N. S. sera 11 free-living jaguars (Panthera onca) two protected areas Pantanal region Mato Grosso state, Brazil. Ten (90.9%) showed seropositivity for eight (72.7%) neurona, seven (63.6%)...

Neosporosis is considered a major cause of abortion among cattle worldwide. The aim this study was to investigate the prevalence anti-Neospora caninum antibodies in dairy and correlate them with possible risk factors on 63 small farms (family farms) municipality Ji-Paraná, main milk-producing region state Rondônia, northern Brazil. For purpose, 621 serum samples were collected from cows evaluated by means indirect fluorescence antibody test (IFAT). overall herd N. (38/63; 60.31%) showed that...

The present study evaluated Canine Monocytic Ehrlichiosis and Thrombocytic Anaplasmosis in 77 dogs treated at the Veterinary Hospital (HOVET) of Federal University Mato Grosso (UFMT) 2009. An association among clinical hematological, serological molecular data was investigated. Dogs were by partial amplification dsb 16S rRNA Ehrlichia canis Anaplasma platys Indirect Fluorescence Antigen Test (IFAT) for E. canis. DNA A. detected 18 (23.3%) 07 (9.1%) respectively. Fifty four (70.1%) positive...

Trypanosoma (Megatrypanum) theileri is a flagellated protozoan that infects ruminants and it displays high genetic diversity. In this study, we investigated the prevalence rates of based on hemoculture molecular diagnosis. The isolates T. thus obtained were characterized by markers SSU rDNA gGAPDH diagnosis Cathepsin L-like gene (PCR-TthCATL). PCR-TthCATL indicated an overall rate 8.13%, CATL derived sequence named IB was identified for first time in cattle western Amazon region, as well IF...

ABSTRACT: The municipality of Ji-Paraná, Rondônia, is one the major dairy production areas in north region Brazil. Thus, it important to evaluate infectious agents that have potential negatively affect productivity industry. objective this study was investigate prevalence ' Candidatus Mycoplasma haemobos' by using a PCR-based detection method and correlate with herd variables (abortion frequency, weak calf birth rate, total cattle number, >24-month-old cow farm size, system) family farms...

Giardia duodenalis, a unicellular, eukaryotic, and flagellated protozoan, presents two evolutionary forms in its life cycle, namely, trophozoites cysts. It causes diarrhea humans, dogs, cats, rodents, ungulates. Despite being morphologically similar, the isolates of G. duodenalis are genetically diverse, affecting stability unanimity taxonomic classification. Since different assemblages may occur within one isolate, multilocus genotyping is recommended for genetic identification.To determine...

Abstract Trypanosomatids are uniflagellate protozoa belonging to the Trypanosomatidae family. The genera Trypanosoma and Leishmania of paramount importance as they contain species that cause serious diseases, such Chagas disease Leishmaniasis, respectively. objective present study was identify trypanosomatids in whole blood free-living captive neotropical primates Mato Grosso State, Midwest Brazil. Between 2017 2019, 38 samples were collected from seven different primate cities state....

Neosporosis is a parasitic disease caused by the obligate intracellular protozoan Neospora caninum, which responsible for miscarriage cases in cattle countrywide. The vertical or transplacental route with prevalent miscarriages between 3rd and 6rd months of pregnancyis main transmission form cattle. aim present study to investigate presence anti-N.caninum antibodies (Bos indicus) Nellore female animals fetuses Nossa Senhora do Livramento County - MT. Blood samples from 489 bovines 128 bovine...

Neosporosis is caused by an obligate intracellular protozoan, Neospora caninum . It considered one of the most widespread and frequent causes abortion in cattle worldwide. To evaluate prevalence anti-N. antibodies associated risk factors, serum samples were collected from 2,452 bovines at 262 farms northern Pantanal, state Mato Grosso, Brazil. Each farmer was asked to fill out a questionnaire for subsequent epidemiological data analysis. Anti-N. detected means indirect immunofluorescent...

Neste trabalho foi realizada uma avaliação sobre a presença de anticorpos anti-Leishmania infantum chagasi em cães domésticos das áreas urbanas e rurais da região do Pantanal brasileiro usando técnicas sorológicas. Um total 429 foram amostrados três bioma Pantanal, incluindo os municípios Poconé, Santo Antônio Leverger Barão Melgaço, Mato Grosso, o município Corumbá, Grosso Sul. A reação imunofluorescência indireta (RIFI) utilizada para detectar (ponto corte 40) Leishmania como antígeno....

This study evaluated species richness and rickettsial infection in ticks infesting non-volant small mammals a preserved area of the Cerrado biome, located region Araguaia Valley, Midwestern Brazil. Between 2017 2018, 116 wild (102 rodents 14 marsupials) were captured 75 collected tested for infection. Four [(Amblyomma auricularium, Amblyomma cajennense sensu stricto (s.s.), coelebs, Ornithodoros guaporensis)] identified with new tick-host association as follows: nymphs A. s.s. auricularium...

In Brazil, the most important tickborne pathogens affecting dogs include Babesia vogeli, Ehrlichia canis, Anaplasma platys, Hepatozoon and Mycoplasma haemocanis. spp. spp., transmitted by ixodid ticks, have been reported to naturally infect are widespread. The authors aimed investigate incidence of B. vogeli infection using molecular methods identify factors associated with in from urban areas Cuiabá municipality, Midwestern Brazil. Polymerase chain reaction (PCR) assay revealed a prevalence...

O estudo teve como objetivo avaliar a ocorrência de anticorpos anti-Rickettsia spp. e anti-Ehrlichia canis, bem detecção molecular DNA Babesia vogeli, Ehrlichia Hepatozoon em cães do município Ji-Paraná, estado Rondônia, norte Brasil. Para tanto, foram analisadas 177 amostras soro sangue por meio reação imunofluorescência indireta (RIFI) cadeia pela polimerase (PCR), respectivamente. Um total 8,47 (15/177) sororreativos para pelo menos uma espécie Rickettsia, com títulos finais variando 64...

Cryptosporidium spp. is a protozoan that infects wide range of vertebrate hosts; it has been reported to be the cause severe illness or death in livestock worldwide, which leads decreased performance and production losses, especially young animals. This study investigated presence calves from beef farms state Mato Grosso, midwestern Brazil. For this purpose, fecal samples 237 animals aged ? 45 days, raised 20 rural properties were subjected DNA extraction nested polymerase chain reaction...

Ehrlichia minasensis, a novel species of that is closely related to E. canis, known infect cattle and deer in Canada. This rickettsial bacterium was isolated from Rhipicephalus (Boophilus) microplus ticks dairy beef southeast midwestern Brazil, respectively. The purpose this study determine the seroprevalence perform molecular detection sp. northern region Brazil. conducted municipality Ji-Paraná, located state Rondônia western Brazilian Amazon region. Blood serum samples were obtained...

We conducted a seroepidemiological study on the occurrence of anti-Sarcocystis spp.and anti-Toxoplasma gondii antibodies in dogs from family farming properties municipality Ji-Paraná, Rondônia.Blood samples were collected apparently healthy between September 2012 and November 2013.In total, 181 blood serum analyzed using an indirect immunofluorescence assay, among which 57 (31.49%) 20 (11.04%) positive for anti-T.gondii spp., respectively.Statistical analyses showed that type food fed to was...