A5074486629- Electron Spin Resonance Studies
- Photosynthetic Processes and Mechanisms
- Advanced NMR Techniques and Applications
- Bacteriophages and microbial interactions
- Heme Oxygenase-1 and Carbon Monoxide
- Advanced MRI Techniques and Applications
- Hemoglobin structure and function
- Spectroscopy and Quantum Chemical Studies
- Nitric Oxide and Endothelin Effects
- Molecular spectroscopy and chirality
Miami University
2020-2025
Cleveland Clinic Lerner College of Medicine
2022
Significance Nitric oxide (NO) performs many biological functions, but how it operates at the molecular and cellular levels is not fully understood. We discovered that cell NO generation physiologic triggers a rapid redeployment of intracellular heme, an iron-containing cofactor, we show this drives assembly natural receptor protein, soluble guanylyl cyclase, which needed for to perform its signaling functions. Our study uncovers way can shape processes cells may use control their...
Membrane proteins play a vital role in various cellular functions and are important targets for drug interactions. However, determining their local secondary structure is challenging due to hydrophobic nature limited experimental techniques. This study focuses on the use of electron spin echo envelope modulation (ESEEM) spectroscopy, combination with site-directed labeling (SDSL) 2H-labeled amino acid side chain (d10-Leu), recently discovered phage-encoded lytic protein, gp28. gp28 membrane...
The bacteriophage infection cycle plays a crucial role in recycling the world's biomass. Bacteriophages devise various cell lysis systems to strictly control length of for an efficient phage life cycle. Phages evolved with protein systems, which can and fine-tune this depending on host growing environment. Among these proteins, holin controls first rate-limiting step by permeabilizing inner membrane at allele-specific time concentration hence known as simplest molecular clock. Pinholin S21...