A5081991967- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neural Engineering
- Retinal Development and Disorders
- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- Clusterin in disease pathology
- Calcium signaling and nucleotide metabolism
- Connexins and lens biology
- Cholinesterase and Neurodegenerative Diseases
- RNA Research and Splicing
- Tryptophan and brain disorders
- Mitochondrial Function and Pathology
- Salivary Gland Tumors Diagnosis and Treatment
- Alzheimer's disease research and treatments
- Ear and Head Tumors
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Ion Channels and Receptors
- Amino Acid Enzymes and Metabolism
- Calcium Carbonate Crystallization and Inhibition
- Amyotrophic Lateral Sclerosis Research
- Electron Spin Resonance Studies
- Copper Interconnects and Reliability
Queen Mary University of London
2022
University of Oxford
2018-2020
Imperial College London
2017-2018
King's College London
1994-2017
Hammersmith Hospital
2017
Wolfson Medical Center
2001-2007
Texas Instruments (United States)
2002
Kanazawa University
1995
University College London
1990-1993
University of Cambridge
1991
Fouling biofilm development was monitored in a completely mixed tubular recycle reactor. A unique sampling system allowed direct (brightfield, epifluorescence, and scanning electron photomicroscopy) indirect (increased fluid frictional resistance) observations of biofilms. Low velocity (138.5 cm/s) experiments had shorter induction times matrixes which included firmly adherent filamentous bacteria. High (265.4 longer with bacteria present only after the accumulation extracellular materials....
Our understanding of the molecular processes underlying Alzheimer's disease (AD) is still limited, hindering development effective treatments and highlighting need for human-specific models. Advances in identifying components amyloid cascade are progressing, including role protein clusterin mediating β-amyloid (Aβ) toxicity. Mutations gene (CLU), a major genetic AD risk factor, known to have important roles Aβ processing. Here we investigate how CLU mediates Aβ-driven neurodegeneration human...
1. The M‐like current IK(M,ng) in differentiated NG108‐15 mouse neuroblastoma x rat glioma hybrid cells has been studied using tight‐seal, whole‐cell patch‐clamp recording. 2. When calculated from steady‐state current‐voltage curves, the conductance underlying showed a Boltzmann dependence on voltage with half‐activation Vo = ‐44 mV (in 3 mM [K+]) and slope factor (a) 8.1 mV/e‐fold increase conductance. In 12 [K+] ‐38 6.9 mV. deactivation reciprocal time constant accelerated...
1. Acetylcholine (ACh) produces two membrane current changes when applied to NG108‐15 mouse neuroblastoma x rat glioma hybrid cells transformed (by DNA transfection) express m1 muscarinic receptors: it activates a Ca(2+)‐dependent K+ conductance, producing an outward current, and inhibits voltage‐dependent conductance (the M conductance), thus diminishing the M‐type (IK(M)) inward current. The present experiments were undertaken find out how far inhibition of IK(M) might be secondary...
1. The possible role of nicotinamide‐adenine dinucleotide (NAD+) and cyclic adenosine diphosphate ribose (cADPR) as regulators M‐type K+ currents (IK(M)) has been studied in whole‐cell patch‐clamped NG108‐15 mouse neuroblastoma x rat glioma cells that had transformed to express m1 muscarinic acetylcholine receptors (mAChRs). 2. Pre‐incubation for 6‐8 h with streptozotocin (2‐5 mM) reduced NAD+ levels by 40‐50%. Nicotinamide increased prevented depletion streptozotocin. 3. Streptozotocin...
Abstract The ability of different genotypic muscarinic acetylcholine receptors (mAChR) to inhibit the neural K + ‐current, I M , was assessed in clones NG108‐15 mouse neuroblastoma × rat glioma cells transfected with DNA for genomic mAChRs ml –4 using tight‐seal, whole‐cell patch clamp recording. No significant inhibition seen native (non‐transfected) cells, or m2 m4 DNA‐transfected at concentrations up 1 mM muscarine 100 μM. Both and produced complete m3 but only partial (50–60%) m1...
Thirty-three cases of chemodectoma are presented, with patients staged in three groups: tympanic (1), tympanomastoid (2), and petrosal-extrapetrosal (3). Surgery is effective group 1 cases. moderate-dose postoperative cobalt irradiation (3,500 to 4,000 rads weeks) used for more advanced lesions higher-dose (5,000 5,500 five reserved very extensive tumors. Overall control tumor these 84% follow-ups from 31 years.
1 We have studied the effect of bradykinin (Bk) on fibroblast-like satellite (FLS) cells isolated from cultures neonatal rat dorsal root ganglia (DRG). 2 In voltage-clamped FLS Bk evoked an inward current response that was concentration dependent with a half-maximal nM. 3 indo-1 AM-loaded rise in intracellular Ca2+ 4 The still produced to absence extracellular but inhibited if EGTA increased 0.5 5 mM, which suggests release and subsequent Ca2+. reversal potential Bk-induced consistent being...
The facial lymph nodes, consisting of the infraorbital, buccinator, and supramandibular subgroups, are present in no more than 30% probably less 20% all individuals. When they do exist, however, play an important role spread diagnosis malignancies. These nodal groups drain anterior superficial structures empty into submaxillary bed. buccinator node is most frequently often involved this group. We have collected ten cases malignancies involving these nodes illustrative case metastatic...
Amyotrophic lateral sclerosis (ALS) is the most common adult-onset neuromuscular disorder characterised by selective loss of motor neurons leading to fatal paralysis. Current therapeutic approaches are limited in their effectiveness. Substantial advances understanding ALS disease mechanisms has come from identification pathogenic mutations dominantly inherited familial (FALS). We previously reported a coding mutation D-amino acid oxidase (DAOR199W) associated with FALS. DAO metabolises...
Each subunit of the Xenopus nucleoplasmin polypeptide possesses a single nuclear location sequence, boundaries which have been determined by deletion analysis. However, sequence identified in this analysis is unable to locate pyruvate kinase cell nucleus. Further investigation revealed that while element not sufficient for localisation, it necessary feature slightly longer sequences can This unusual suggests testable models interaction with transport mechanism, one discussed here.