Strawberry is ranked third in the value of production crops Florida, USA. Classifying strawberry maturity and monitoring growth status field very critical for accurate yield prediction, efficient management, achieving highest crop production. The traditional method distinguishing based on either physical appearance or internal chemical substance content. However, time-consuming costly. In this research, an automatic classification system was developed rapid different stages. A...

We present viral evolution as a novel and powerful method to optimize non-viral proteins. used this approach the tetracycline (Tc)-regulated gene expression system (Tet system) for its function in mammalian cells. The components of Tet were incorporated human immunodeficiency virus (HIV)-1 such that replication is controlled by regulatory system. Upon long term HIV-rtTA T cells, we obtained variant with an enhanced potential resulting from improved rtTA component introduced identified single...

Abstract An objective requirement of the sustainable utilization soil resources and agricultural production management is determination status salinization an understanding its dynamics. This study selected coastal plain, Yellow River flood key inland areas with in Shandong Province. Sampling analysis cultivated lands were conducted 2015–2016, 3,551 valid samples obtained. Statistical spatial analyses performed compared data from Second Soil Survey (1979–1985). The degree, area,...

The doxycycline (dox)-inducible Tet-On system is widely used to control gene expression in mammalian cells. This based on the bacterial Tet operon, which has been modified and improved for its function eukaryotic To identify optimal different applications, we compared variants frequently cell types that were either transiently transfected with relevant plasmids or stably transduced an "all-in-one" lentiviral vector. V10 variant performed optimally cells demonstrated no background activity...

Strawberries are an important and high-value crop in the United States. The total value of strawberry production States was $3.42 billion 2021. However, a major pest strawberries, two-spotted spider mites (TSSM) (Tetranychus urticae Koch), can cause severe economic losses due to feeding on chlorophyll which interferes with photosynthesis. Predatory (Neoseiulus californicus Phytoseiulus persimilis) control TSSM populations provide alternative conventional pesticides. more friendly environment...

Abstract Background We have previously constructed a doxycycline (dox)-dependent HIV-1 variant by incorporating the Tet-On gene regulatory system into viral genome. Replication of this HIV-rtTA virus is driven dox-inducible transactivator protein rtTA, and can be switched on off at will. proposed conditional-live as novel vaccine approach against HIV-1. Upon vaccination, replication temporarily activated transient dox administration controlled to extent needed for optimal induction immune...

Live attenuated human immunodeficiency virus type 1 (HIV-1) vaccines are considered unsafe because more quickly replicating pathogenic variants may evolve after vaccination. As an alternative vaccine approach, we have previously presented a doxycycline (dox)-dependent HIV-1 variant that was constructed by incorporating the tetracycline-inducible gene expression system (Tet-On system) into viral genome. Replication of this HIV-rtTA is driven dox-inducible transcriptional activator rtTA and...

Doxycycline (DOX) is widely used as a pharmacological agent and an effector molecule in inducible gene expression systems. For most applications, it important to determine whether the DOX concentration reaches level required for optimal efficacy. We developed sensitive bioassay measuring biological samples. modified HeLa cell line with luciferase reporter under control of DOX-inducible Tet-On system regulation expression. These HeLaDOX cells constitutively express novel variant rtTA...

The Tet-Off (tTA) and Tet-On (rtTA) regulatory systems are widely applied to control gene expression in eukaryotes. Both based on the Tet repressor (TetR) from transposon Tn10, a dimeric DNA-binding protein that binds specific operator sequences (tetO). To allow independent regulation of multiple genes, novel being developed respond different effectors bind tetO sites. prevent heterodimerization when expressed same cell, single-chain variants transactivators have been constructed....

Deinococcus wulumuqiensis R12, which was isolated from arid irradiated soil in Xinjiang province of China, belongs to a genus that is well-known for its extreme resistance ionizing radiation and oxidative stress. The DNA-binding protein Dps has been studied great contribution resistance. To explore the role D. sequence homology-modeled structure were analyzed. In addition, dps gene knocked out proteomics used verify functions R12. Docking data DNA binding experiments vitro showed R12 better...

Lower respiratory tract infections (LRTIs) are one of the leading causes hospital admissions among children. In this study, we aimed to describe epidemiological characteristics viral pathogens associated with LRTIs in hospitalized children Yan'an; has yet be reported literature and may guide public health interventions resource allocation region. Between June 2021 May 2023, conducted a retrospective analysis results detection using oral pharyngeal swabs from 4565 Inpatient Department Yan'an...

This paper describes the system that we use for Chinese segmentation task in 3rd CIPS-SIGHAN bakeoff.We character sequence labeling method segmentation, and order to improve accuracy over multi-domain, present a CRF-based integrating supervised, unsupervised lexical features.We firstly preliminarily segment target data using CRF model trained three types of features mentioned above, from result which new words are detected absorbed into lexicon.To generalize across different domains, then...

The ecological environment of the Yellow River Delta is fragile, and soil degradation in region serious. Therefore it important to discern status a timely manner for conservation utilization. study area this was Kenli County China. First, physical chemical data were obtained by field investigations sample analyses, hyper-spectra air-dried samples via spectrometer. Then, index (SDI) constructed key indicators degradation, including pH, SSC, OM, AN, AP, AK, texture. Next, according cluster...

Interference detection using deep neural network has recently received increasing attention due to its capability in learning rich features of data. In this paper, we proposed a low-complexity blind interference method. Our method operates on Time-frequency overlapped signal and can separate which from signal. The uses AutoEncoder reconstruct the transmitted consists several layers recurrent (RNN) is well-suited for representations time-correlated Simulation results show that separated same...