A5103162703- Cancer-related Molecular Pathways
- Adenosine and Purinergic Signaling
- Ubiquitin and proteasome pathways
- Histone Deacetylase Inhibitors Research
- Biochemical and Molecular Research
- Amino Acid Enzymes and Metabolism
- Cell death mechanisms and regulation
- Pancreatic function and diabetes
- Carbohydrate Chemistry and Synthesis
- Mitochondrial Function and Pathology
- Sesquiterpenes and Asteraceae Studies
- Biochemical effects in animals
- Cancer, Hypoxia, and Metabolism
- Protein Degradation and Inhibitors
- Glycosylation and Glycoproteins Research
- Cannabis and Cannabinoid Research
- Natural product bioactivities and synthesis
- Reproductive Biology and Fertility
- Endoplasmic Reticulum Stress and Disease
- Retinoids in leukemia and cellular processes
- Polyamine Metabolism and Applications
- Metabolism and Genetic Disorders
- Nuclear Receptors and Signaling
- Pharmacological Effects of Natural Compounds
- Adipose Tissue and Metabolism
Sapienza University of Rome
2024-2025
Temple University
2013-2016
University of Palermo
1994-2013
Clinica Luganese Moncucco
2008-2010
European Institute of Oncology
2005
Casa di Cura Columbus
1999
Istituto Zooprofilattico Sperimentale Della Sicilia
1994
Istituto di Chimica Biomolecolare
1972-1974
Triple-negative breast cancers (TNBCs) are clinically aggressive forms associated with a poor prognosis. We evaluated the cytotoxic effect exerted on triple-negative MDA-MB231 cancer cells both by parthenolide and its soluble analogue dimethylamino (DMAPT) explored underlying molecular mechanism. The drugs induced dose- time-dependent decrement in cell viability, which was not prevented caspase inhibitor z-VAD-fmk. In particular first hours of treatment (1–3 h), DMAPT strongly stimulated...
Abstract Triple-negative breast cancers (TNBCs) are aggressive forms of carcinoma associated with a high rate recidivism. In this paper, we report the production mammospheres from three lines TNBC cells and demonstrate that both parthenolide (PN) its soluble analog dimethylaminoparthenolide (DMAPT) suppressed induced cytotoxic effects in cancer stem-like cells, derived dissociation mammospheres. particular, drugs exerted remarkable inhibitory effect on viability cells. Such an was by N...
Several mutations in the SOD1 gene encoding for antioxidant enzyme Superoxide Dismutase 1, are associated with amyotrophic lateral sclerosis, a rare and devastating disease characterized by motor neuron degeneration patients' death within 2–5 years from diagnosis. Motor loss related symptomatology manifest mostly adult life and, to date, there is still gap of knowledge on precise cellular molecular events preceding neurodegeneration. To deepen our awareness early phases disease, we leveraged...
Abstract Vitamin B6 is a water-soluble vitamin which possesses antioxidant properties. Its catalytically active form, pyridoxal 5’-phosphate (PLP), crucial cofactor for DNA and amino acid metabolism. The inverse correlation between cancer risk has been observed in several studies, although dietary intake sometimes failed to confirm this association. However, the molecular link remains elusive. Previous work shown that deficiency causes chromosome aberrations (CABs) Drosophila human cells,...
In this article, we demonstrate that the synthetic cannabinoid <i>R</i>-(+)-(2,3-dihydro-5-methyl-3-[(4-morpholinyl)methyl]pyrol[1,2,3-de]-1,4-benzoxazin-6-yl)-(1-naphthalenyl) methanone mesylate (WIN 55,212-2) sensitizes human hepatocellular carcinoma (HCC) cells to apoptosis mediated by tumor necrosis-related inducing ligand (TRAIL). The apoptotic mechanism induced treatment with WIN/TRAIL combination involved loss of mitochondrial transmembrane potential and led activation caspases. HCC...
Abstract Introduction Pyridoxal 5’-phosphate (PLP), the biologically active form of vitamin B6 is involved in 4% cellular enzymatic activities and its deficiency responsible for or contributes to several human diseases. The study underlying mechanisms still infancy requires suitable model organisms. In Drosophila produces chromosome aberrations hallmarks diseases including diabetes cancer. However, effects have never been examined at a metabolic level. Objectives This evaluates changes...
This report is focused on the apoptotic effect induced by MG132, an inhibitor of 26S proteasome, in human hepatoma HepG2 cells. The results were compared with those obtained non-transformed Chang liver MG132 reduced viability cells a time- and dose-dependent manner. was tight connection induction apoptosis, as indicated fluorescence microscopy cytometric analysis, accompanied remarkable increase production H2O2 reduction mitochondrial transmembrane potential (Δψm). In addition cell death...
This report shows that histone deacetylase inhibitors (HDACIs) induced apoptosis in human hepatoma HepG2 cells a dose- and time-dependent manner. Trichostatin A (TSA), ITF2357 suberoylanilide hydroxamic acid (SAHA), which were very effective agents, caused apoptotic effects after lag phase of 12-16 h. In order to elucidate the mechanism HDACIs action we have studied TSA, SAHA on acetylation p53 histones H2A, H2B, H3 H4. It was observed rapidly these proteins, being clearly visible already at...
The sesquiterpene lactone parthenolide (PN) has recently attracted considerable attention because of its anti-microbial, anti-inflammatory and anticancer effects. However, the mechanism cytotoxic action on tumor cells remains scarcely defined. We provided evidence that effect exerted by PN in MDA-MB-231 breast cancer was mediated production reactive oxygen species (ROS). present study shows promoted phosphorylation EGF receptor (phospho-EGFR) at Tyr1173, an event which observed already 1 h...
This study demonstrated that cisplatin and carboplatin stimulate apoptosis in human retinoblastoma Y79 cells, being the most effective compound. The apoptotic effect appeared after 8 h then increased a time-dependent manner. Treatment with also provoked an increase level of p53 p21, lowering Bcl-2. prolonged exposure cells to induced resistance cisplatin, etoposide. basal was resistant higher than untreated while Bcl-2 not modified. levels did change treating or However, camptothecin which...
This study describes the molecular mechanism by which treatment with 3‐AB, a potent inhibitor of PARP, allows human osteosarcoma MG‐63 cells to restrict growth and enter differentiation. Our findings show that in cells, aberrant gene expression keeps Rb protein constitutively inactivated through hyperphosphorylation this promotes uncontrolled proliferation cells. After 3‐AB‐treatment, poly(ADP‐ribosyl)ation nuclear proteins markedly decreases results an increase both hypophosphorylated...
Abstract Polyamines and related monoacetyl derivatives were studied in chick embryo retina during development (6th–19th day). Putrescine, which is high the first phase of retinogenesis, necessary to sustain both tissue proliferation via N ‐acetylputrescine, γ‐aminobutyric acid synthesis. A later increase spermidine particularly spermine may play a role last when reaches maturation. The presence ‐acetylspermidine already at 8th day indicates that retina, putrescine synthesis can depend on two...