A5049747639- Advanced biosensing and bioanalysis techniques
- Click Chemistry and Applications
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
- Luminescence and Fluorescent Materials
- Molecular Junctions and Nanostructures
- DNA and Nucleic Acid Chemistry
- Advanced Biosensing Techniques and Applications
- Photochemistry and Electron Transfer Studies
- Lanthanide and Transition Metal Complexes
- Protein purification and stability
- Dendrimers and Hyperbranched Polymers
- Genetics, Bioinformatics, and Biomedical Research
- Molecular Biology Techniques and Applications
National Academy of Sciences of Belarus
2017-2021
Institute of Physical and Organic Chemistry
2012-2021
We developed a novel technique for the efficient conjugation of oligonucleotides with various alkyl azides such as fluorescent dyes, biotin, cholesterol, N-acetylgalactosamine (GalNAc), etc. using copper-catalysed alkyne–azide cycloaddition on solid phase and CuI·P(OEt)3 catalyst. Conjugation is carried out in an oligonucleotide synthesizer fully automated mode coupled to synthesis on-column deprotection. also suggest set reagents construction diverse conjugates. The sequential double-click...
GalNAc conjugation is emerging as a dominant strategy for delivery of therapeutic oligonucleotides to hepatocytes. The structure and valency the ligand contributes potency conjugates. Here we present panel multivalent variants using two different synthetic strategies. Specifically, novel conjugate based on support-bound trivalent cluster, four others phosphoramidite monomer that was readily assembled into tri- or tetravalent designs during solid phase oligonucleotide synthesis. We compared...
Fluorescent properties of molecular beacons with two dyes or quenchers in real-time PCR are reported.
An azido-derivative of a fluorescein bifluorophore was obtained and used for the synthesis "molecular beacon"-type oligonucleotide fluorogenic probes RT-PCR. Eight probe variants were synthesized based on an optimized sequence: with one or two quencher residues at 3'-end, single label attached to 5'-end using modifying phosphoramidites (short linker) "click reaction" (long linker). Comparison in RT-PCR showed that doubled (single short dye linker dye) are somewhat superior sensitivity...
Polyethyleneglycol (PEG) is nontoxic, nonimmunogenic, hydrophilic, chargeless and nonbiodegradable poliymer. Its usage as a part of therapeutics protein drugs common in medicine practice. It known that covalent attachment PEG conduces to prolong blood circulation half-lives, improves drug solubility stability reduces immunogenicity. allows optimizing pharmacodynamic pharmacokinetic properties. The goal structure optimization therapeutic proteins conjugates with reduce loss biological...