A5092500527- Chemical Synthesis and Analysis
- Carbohydrate Chemistry and Synthesis
- vaccines and immunoinformatics approaches
- SARS-CoV-2 and COVID-19 Research
- Analytical Chemistry and Chromatography
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Protein Structure and Dynamics
- Viral Infections and Immunology Research
- Chromosomal and Genetic Variations
- Enzyme Structure and Function
- COVID-19 Clinical Research Studies
- RNA and protein synthesis mechanisms
École Polytechnique Fédérale de Lausanne
2023-2024
University of Lausanne
2023
Protein structures are essential to understanding cellular processes in molecular detail. While advances artificial intelligence revealed the tertiary structure of proteins at scale, their quaternary remains mostly unknown. We devise a scalable strategy based on AlphaFold2 predict homo-oligomeric assemblies across four proteomes spanning tree life. Our results suggest that approximately 45% an archaeal proteome and bacterial 20% two eukaryotic form homomers. predictions accurately capture...
Gene duplication and domain acquisition are key mechanisms driving protein diversification. However, the impact of these processes on function evolution transcription factors (TFs) remains poorly characterized. Here, we show that DUF3669 domain, found in a subset KRAB zinc-finger proteins (KZFPs), originated from co-option fragment LINE-1 transposable element ORF1p. Similar to its LINE-1-encoded ancestor, promotes KZFPs trimerization enables formation nuclear condensates with...
Biocatalytic hydroamination of alkenes is an efficient and selective method to synthesize natural unnatural amino acids. Phenylalanine ammonia-lyases (PALs) have been previously engineered access a range substituted phenylalanines heteroarylalanines, but their substrate scope remains limited, typically including only arylacrylic Moreover, the enantioselectivity in electron-deficient substrates often poor. Here, we report structure-based engineering PAL from Planctomyces brasiliensis (PbPAL),...
Due to the rapid evolution of SARS-CoV-2 variants with reduced sensitivity vaccine-induced humoral immunity and near complete loss protective efficacy licensed therapeutic monoclonal antibodies, we isolated a potent, broad-spectrum neutralizing antibody that could potentially provide prophylactic protection immunocompromised patient populations.
Abstract Biocatalytic hydroamination of alkenes is an efficient and selective method to synthesize natural unnatural amino acids. Phenylalanine ammonia‐lyases (PALs) have been previously engineered access a range substituted phenylalanines heteroarylalanines, but their substrate scope remains limited, typically including only arylacrylic Moreover, the enantioselectivity in electron‐deficient substrates often poor. Here, we report structure‐based engineering PAL from Planctomyces brasiliensis...
Abstract The rapid evolution of SARS-CoV-2 to variants with improved transmission efficiency and reduced sensitivity vaccine-induced humoral immunity has abolished the protective effect licensed therapeutic human monoclonal antibodies (mAbs). To fill this unmet medical need protect vulnerable patient populations, we isolated P4J15 mAb from a previously infected, vaccinated donor, <20 ng/ml neutralizing activity against all Omicron including latest XBB.2.3 EG.1 sub-lineages. Structural...