A5047753228- Circular RNAs in diseases
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Cancer, Hypoxia, and Metabolism
- Extracellular vesicles in disease
- Pulmonary Hypertension Research and Treatments
- Ferroptosis and cancer prognosis
- Education and Work Dynamics
- Radiopharmaceutical Chemistry and Applications
- Chemical Synthesis and Reactions
- Synthesis and Biological Activity
- HVDC Systems and Fault Protection
- ATP Synthase and ATPases Research
- Phytochemical Studies and Bioactivities
- Mitochondrial Function and Pathology
- Bioactive Compounds and Antitumor Agents
- Microbial Natural Products and Biosynthesis
- Traditional and Medicinal Uses of Annonaceae
- Synthesis and Catalytic Reactions
- Oxidative Organic Chemistry Reactions
- Frequency Control in Power Systems
- RNA Research and Splicing
- Marine Sponges and Natural Products
- Eicosanoids and Hypertension Pharmacology
Harbin Medical University
2004-2025
Daqing City People's Hospital
2022-2024
Ministry of Education of the People's Republic of China
2023
Marquette University
2018
Milwaukee VA Medical Center
2018
Second Affiliated Hospital of Harbin Medical University
2013
Background: Circular RNAs (circRNAs) have been implicated in pulmonary hypertension progression through largely unknown mechanisms. Pulmonary artery endothelial cell (PAEC) dysfunction is a hallmark the pathogenesis of hypertension. However, specific role circular PAEC injury caused by hypoxia remains unclear. Methods: In this study, using Western blotting, RNA pull down, Dual-luciferase reporter assay, immunohistochemistry, and immunofluorescence, we identified novel derived from...
We explored mechanisms that alter mitochondrial structure and function in pulmonary endothelial cells (PEC) after hyperoxia.Mitochondrial structures of PECs exposed to hyperoxia or normoxia were visualized fragmentation quantified. Expression pro-fission fusion proteins autophagy-related assessed by Western blot. Mitochondrial oxidative state was determined using mito-roGFP. Tetramethylrhodamine methyl ester estimated polarization treatment groups. The role mitochondrially derived reactive...
ABSTRACT Long noncoding RNAs (lncRNAs) are implicated in pulmonary hypertension (PH) progression. However, the underlying mechanisms remain largely unknown. Although mitophagy plays a crucial role hypoxia‐induced PH pathogenesis, of lncRNAs remains unclear. Especially, mechanism lncRNA encoded by mitochondrial genome regulating needs to be elucidated. We explored lncND5 human artery smooth muscle cells (PASMCs) and Sugen5416 plus hypoxia (SuHx)‐induced mouse model vitro vivo. LncND5...
Abstract Glycolysis is a major determinant of pulmonary artery smooth muscle cell (PASMC) proliferation in hypertension (PH). Circular RNAs (circRNAs) are powerful regulators glycolysis multiple diseases; however, the role circRNAs PH has been poorly characterized. The aim this study was to uncover regulatory mechanism new circRNA, circNAP1L4, human (HPASMC) through host protein NAP1L4 regulate super‐enhancer‐driven gene hexokinase II (HK II). CircNAP1L4 downregulated hypoxic HPASMCs and...
Pulmonary hypertension is a rare, progressive disorder that can lead to right ventricular hypertrophy, heart failure, and even sudden death. N6-methyladenosine modification the main methyltransferase mediates it, methyltransferase-like (METTL) 3, exert important effects on many biological pathophysiological processes. However, role of METTL3 in pyroptosis remains unclear.
BACKGROUND: Ferroptosis has been implicated in pulmonary hypertension. Chromatin-associated RNAs are linked to ferroptosis. However, their role vascular ferroptosis PH is unexplored. METHODS: Bioinformatics, Sanger sequencing, RNase R and others were employed identify differentially higher expression of ca-circSCN8A. Functional gain loss assays unveil the ca-circSCN8A hypoxia-induced redox-dependent HPASMCs mice model. Interaction between FUS was detected via RNA immunoprecipitation...
BACKGROUND: Pulmonary hypertension (PH) is a rare and fatal disease, the pathological changes of which include pulmonary arterial smooth muscle cell (PASMC) proliferation, basis vascular remodeling. Studies have demonstrated that chromatin-associated circRNA can regulate variety biological processes, including PASMC proliferation in patients with hypoxic PH. However, role PH remains largely unexplored. In this study, we aimed to identify function mechanism METHODS: The ca-circFOXP1 was...
BACKGROUND: Ferroptosis has been implicated in pulmonary hypertension (PH), and chromatin-associated RNAs are increasingly recognized as key regulators of this process. However, the detailed mechanism remains unexplored. METHODS: Bioinformatics, Sanger sequencing, RNase R digestion were used to identify upregulation ca-circSCN8A. Functional gain loss assays unveil role ca-circSCN8A hypoxic redox–dependent ferroptosis human arterial smooth muscle cells a PH mice model. Interaction between FUS...
BACKGROUND: Pulmonary artery endothelial cell (PAEC) dysfunction is a pathological hallmark of pulmonary hypertension (PH). Yet, the roles long noncoding RNAs (lncRNAs) driven by super-enhancers (SEs) in PAECs are not well understood. In this study, we focused on PAEC-specific SE-associated lncRNA HCG20 (HLA complex group 20) and to elucidate its role underlying mechanisms progression PH. METHODS: ChIP-qPCR, chromosome conformation capture followed PCR, CRISPR/Cas9 (clustered regularly...
A mild, reagent-cyanide-free, and efficient synthesis of O-phosphinoyl-protected cyanohydrins from readily available α-substituted malononitriles was realized using diarylphosphine oxides in the presence O2. Mechanistic studies indicated that addition to initial aerobic oxidation malononitrile derivative notable features this process include formation a tetrahedral intermediate subsequent intramolecular rearrangement. The phosphinoyl-protecting group can be removed by alcoholysis or...
Chemical investigation of the ethanol extract aerial parts Hydrangea macrophylla collected in Sichuan Province China resulted isolation two new cyanogenic glucosides. Their structures were elucidated as [(2R)-2-(b-D-glucopyranosyloxy)-2-(3,4-dimethoxy-phenyl)] acetonitrile (1) and {(2R)-2-[a-D-glucopyranosyl(1®6)b-D-glucopyranosyloxy]-2-(3-hydroxy-4-methoxy-phenyl)}acetonitrile (2) on basis extensive spectroscopic analysis (1D, 2D NMR HRESIMS) chemical studies.
ABSTRACT BACKGROUND Pulmonary hypertension (PH) represents a significant cardiovascular disorder marked by both functional and structural alterations within the pulmonary vasculature. Long non-coding RNAs (lncRNAs) have been closely associated with pathogenesis progression of PH. Nonetheless, precise mechanisms which lncRNAs interact its downstream target molecules to modulate disease remain inadequately elucidated. METHODS The expression levels LINC00599 were quantified in lung tissues mice...