A5103261200- Immunotherapy and Immune Responses
- Dermatology and Skin Diseases
- T-cell and B-cell Immunology
- Allergic Rhinitis and Sensitization
- Thyroid Cancer Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Food Allergy and Anaphylaxis Research
- Nonmelanoma Skin Cancer Studies
- Mast cells and histamine
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Molecular Biology Techniques and Applications
- Tumors and Oncological Cases
- Cancer-related Molecular Pathways
- Cell Adhesion Molecules Research
- Prostate Cancer Diagnosis and Treatment
- Insurance, Mortality, Demography, Risk Management
- Cancer and Skin Lesions
- demographic modeling and climate adaptation
- BRCA gene mutations in cancer
- Cancer Immunotherapy and Biomarkers
- Lymphatic System and Diseases
- Cutaneous Melanoma Detection and Management
- Prostate Cancer Treatment and Research
- Histiocytic Disorders and Treatments
Dermatologie Freiburg
2018-2022
Massachusetts General Hospital
2012-2019
Harvard University
2011-2019
University Medical Center Hamburg-Eppendorf
2015
Universität Hamburg
2015
Indiana University – Purdue University Indianapolis
2013
Beth Israel Deaconess Medical Center
2004-2013
University of Pittsburgh
2013
University of Alabama at Birmingham
2013
City Of Hope National Medical Center
2013
Store-operated Ca2+ entry is mediated by release-activated (CRAC) channels following release from intracellular stores. We performed a genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins that inhibit store-operated influx. A secondary patch-clamp identified CRACM1 and CRACM2 (CRAC modulators 1 2) as of CRAC currents. characterized the human ortholog CRACM1, plasma membrane-resident protein encoded gene FLJ14466. Although overexpression did not affect currents,...
Recent studies have described an encapsulated and infiltrative form of follicular variant papillary thyroid carcinoma (FVPTC). While tumors been reported to virtually no metastatic potential or recurrence risk if angioinvasion capsular penetration are absent, found a significant recurrence. In our experience, substantial number FVPTCs neither fully nor infiltrative, but instead partially-encapsulated (PE) well-circumscribed (WC). Thus, the aim this study was investigate PE/WC in comparison...
Purpose: Targeting mutations leading to PI3K/mTOR/Akt activation are of interest in thyroid cancer. We evaluated the efficacy everolimus aggressive, radioactive iodine-refractory (RAIR) cancer and correlated tumor mutational profiling with response. Exploratory medullary anaplastic cohorts were included.Experimental Design: This single-arm, multi-institutional phase II study was conducted from 2009 2013 patients incurable RAIR who had radiographic progression six months prior enrollment. The...
High-grade neuroendocrine carcinoma of the head and neck is an aggressive neoplasm which rarely arises in oropharynx. Here we report a series 8 oropharyngeal carcinomas associated with both human papillomavirus (HPV) infection tobacco exposure. The tumor occurred predominantly male patients (6 8) at mean age 59 years. Histologically, these cases were all classified as poorly differentiated (small cell carcinoma) high mitotic activity [mean 53.3 mitoses per 10 HPF], necrosis,...
Abstract Mast cell (MC) activation through the high-affinity IgE receptor FcεRI leads to release of mediators involved in immediate-type allergic reactions. Although Abs against tetraspanins CD63 and CD81 inhibit FcεRI-induced MC degranulation, intrinsic role these molecules is unknown. In MCs, expressed at surface lysosomes (particularly secretory that contain mediators). this study, we investigated using a knockout mouse model. CD63-deficiency did not affect vivo numbers tissue...
HOXB13 is a prostate cancer susceptibility gene which shows predisposing (G84E) mutation in 0.1-0.6% of males. We analyzed the prognostic impact expression by immunohistochemistry on tissue microarray containing more than 12,400 cancers. Results were compared to tumor phenotype, biochemical recurrence, androgen receptor (AR) and specific antigen (PSA) as well molecular subtypes defined ERG status genomic deletions 3p, 5q, 6q, PTEN. immunostaining was detectable 51.7% 10,216 interpretable...
Although the lip is considered a high-risk location in cutaneous squamous cell carcinoma (cSCC), it has not been established whether this risk stems from vermilion or locations both.To compare differences risks of recurrence, metastasis, and death cSCCs on vs lip.Retrospective cohort study 303 patients with 310 primary (138 cutaneous, 172 vermilion) diagnosed between 2000 2015 at 2 academic tertiary care centers Boston, Massachusetts.Development local nodal distant disease-specific death,...
We studied expression of the 5-lipoxygenase (5-LO) pathway in normal human skin. In situ hybridization revealed a 5-LO mRNA-containing epidermal cell (EC) population that was predominantly located midportion spinous layer, outer hair root sheaths, and epithelial compartment sebaceous glands. Examination skin specimens by immunohistochemistry primary ECs flow cytometry mapped protein exclusively to Langerhans cells (LCs). The LC largely found nuclear matrix, envelopes, perinuclear regions as...
To cite this article: Peng WM, Yu CF, Kolanus W, Mazzocca A, Bieber T, Kraft S, Novak N. Tetraspanins CD9 and CD81 are molecular partners of trimeric FcɛRI on human antigen-presenting cells. Allergy 2011; 66: 605–611. Abstract Background: Most functions tetraspanins not related to cell-surface receptor ligand binding, but mediated by direct interactions with their partner proteins. Functions FcɛRI, expressed cells (APCs), range from amplification allergic inflammatory reactions active...
Angiotropism is the histopathological correlate of pericytic mimicry and extravascular migratory metastasis (EVMM), a mechanism melanoma spread by migration along external surface blood lymphatic vessels. The frequency angiotropism in primary cutaneous clinical utility its detection remain unclear.We investigated 179 melanomas hematoxylin eosin (H&E), CD31, S100/D240 stains.We detected 31 cases (17%) H&E. CD31 immunohistochemistry increased to 59 (33%). When vessels were included using...
Cutaneous squamous cell carcinomas mainly affect older, predominantly male patients. Most are due to chronic ultraviolet exposure, and associated with actinic keratoses. On the lower extremities, they occur more commonly in women. However, data on these tumors as a distinct group scarce. We evaluated 61 of extremities. Overall, 69% patients were female. Mean age was 75 years. More than 90% well differentiated, 3% showed perineural invasion, none lymphovascular invasion. In all, 63.9%...
The kinase p38α restrains skin stem cell–associated tumorigenesis by inhibiting p63.
9564 Background: ICI has shown major benefit in cutaneous malignancies including melanoma and Merkel cell carcinoma. Efficacy data cSCC is, however, limited. Here we report our institutional experience of anti-PD-1 therapy patients (pts) with advanced cSCC, biomarker analysis. Methods: We conducted a retrospective analysis pts treated nivolumab or pembrolizumab at Massachusetts General Hospital. NGS IHC for PD-L1 expression immune composition were performed, correlation response was...
We describe a sequential multistaining protocol for immunohistochemistry, immunofluorescence and CyTOF imaging formalin-fixed, paraffin-embedded specimens (FFPE) in the formalin gas-phase (FOLGAS), enabling multistaining, independent from primary secondary antibodies retrieval. Histomorphologic details are preserved, crossreactivity loss of signal intensity not detectable. Combined with DAB-based hydrophobic masking metal-labeled antibodies, FOLGAS allows extended use FFPE sections.