A5035343541- Fibromyalgia and Chronic Fatigue Syndrome Research
- Reproductive System and Pregnancy
- Sperm and Testicular Function
- Muscle metabolism and nutrition
- Exercise and Physiological Responses
- Platelet Disorders and Treatments
- Pharmacological Effects and Assays
- Genetic Neurodegenerative Diseases
- Ectopic Pregnancy Diagnosis and Management
- Bacterial biofilms and quorum sensing
- Myofascial pain diagnosis and treatment
- Pregnancy and preeclampsia studies
- Reproductive Biology and Fertility
- RNA Interference and Gene Delivery
- Urological Disorders and Treatments
- Temporomandibular Joint Disorders
- Infant Nutrition and Health
- Vector-borne infectious diseases
- Male Reproductive Health Studies
- Xenotransplantation and immune response
- Renal Diseases and Glomerulopathies
- Urologic and reproductive health conditions
- Reproductive Physiology in Livestock
- Antimicrobial Resistance in Staphylococcus
- Ovarian function and disorders
University College London Hospitals NHS Foundation Trust
2020-2024
University College London
2020-2024
UCL Biomedical Research Centre
2024
University of Bristol
2021-2023
University of Newcastle Australia
2008-2020
Stellenbosch University
2020
University of Liverpool
2020
Brooke Army Medical Center
2016
Vanderbilt University Medical Center
2012
The University of Sydney
1982-2007
Gene therapy for kidney diseases has proven challenging. Adeno-associated virus (AAV) is used as a vector gene targeting other organs, with particular success demonstrated in monogenic diseases. We aimed to establish the by disease of podocyte. The most common cause childhood genetic nephrotic syndrome mutations podocyte NPHS2 , encoding podocin. AAV-based rescue this defect human and mouse models disease. In vitro transduction studies identified AAV-LK03 serotype highly efficient transducer...
Full blood counts, ESR, CRP, haematinics and markers for oxidative stress were measured 33 patients diagnosed with chronic fatigue syndrome (CFS) 27 age sex matched controls. All participants also completed symptom questionnaires. CFS had increases in malondialdehyde (P <0.006), methaemoglobin <0.02), mean erythrocyte volume <0.02) 2,3-diphosphoglycerate <0.04) compared Multiple regression analysis found to be the principal component that differentiated between control subjects....
This study investigated whether alterations in environmental conditions would induce the formation of small colony variant phenotypes (SCV) with associated changes cell morphology and ultra-structure S. aureus, s. epidermidis, lugdunensis. Wild-type clinical isolates were exposed to low temperature (4°C), antibiotic stress (penicillin G vancomycin; 0-10,000 µg mL-1), pH (pH 3-9) osmotic challenge (NaCl concentrations 0-20%). Changes diameter, cell-wall thickness, population distribution (n ≥...
The effect of temperature fluctuation is an important factor in bacterial growth especially for pathogens such as the staphylococci that have to remain viable during potentially harsh and prolonged transfer conditions between hosts. aim this study was investigate response S. aureus, epidermidis, lugdunensis when exposed low (4°C) periods, how affected their subsequent growth, colony morphology, cellular ultra-structure, amino acid composition non-cytoplasmic hydrolysate fraction. Clinical...
Summary. SCA has been identified as an iron-binding protein present in seminal plasma. It electrophoretic mobility similar to transferrin, and different from lactoferrin, whereas it shares immunological characteristics with lactoferrin not possessed by transferrin. The name scaferrin is proposed for this compound.
The excretion of amino acids in urine represents an important avenue for the loss key nutrients. Some such as glycine and histidine are lost higher abundance than others. These two perform physiological functions required synthesis proteins haemoglobin collagen. Stage 1 this study involved healthy subjects (n = 151) who provided first morning samples completed symptom questionnaires. Urine was analysed acid composition by gas chromatography. 2 a subset initial cohort 37) 30 day trial...
SUMMARY The low affinity IgE receptor FcɛRII (CD23) is important in several aspects of T and B cell function. In this study serum levels soluble CD23 (sCD23) were measured three groups: 26 female patients with systemic lupus erythematosus (SLE), 21 females primary Sjögren's syndrome (pSS) 25 normal healthy females. concentration sCD23 was determined using an enhanced chemiluminescent sandwich ELISA developed laboratory. Increased observed pSS SLE compared controls (median 23·0 versus 8·6, P...
Sweat contains amino acids and electrolytes derived from plasma athletes can lose 1-2L of sweat per hour during exercise. may also contain contributions as well urea, sodium potassium the natural moisturizing factors (NMF) produced in stratum corneum. In preliminary experiments, one participant was tested on three separate occasions to compare composition with surface water washings same area skin assess NMF. Two participants performed a 40 minute self-paced cycle session collected cleansed...
Podocyte loss or injury is one of the earliest features observed in pathogenesis diabetic kidney disease (DKD), which leading cause end-stage renal failure worldwide. Dysfunction IGF axis, including binding proteins (IGFBPs), associated with DKD, particularly early stages progression. The aim this study was to investigate potential roles IGFBPs development type 2 focusing on podocytes.IGFBP expression analysed Pima DKD cohort, alongside data from Nephroseq database, and ex vivo human...
Abstract A study was undertaken to assess the ability of erythrocyte protect other tissues against oxidative damage. Radiolabelled (51Cr) human umbilical vein endothelial cells (HUVEC) were incubated with erythrocytes and neutrophils activated phorbol myristate acetate (PMA). Damage indicated by release radioactivity into suspending medium. We found that co‐incubation HUVEC an increasing range concentrations resulted in a dose‐dependent reduction radioactivity. When superoxide cross membrane...
ABSTRACT: This report describes the cytotoxic properties of human seminal plasma and demonstrates that inhibition response to mitogens shown by murine lymphocytes in presence whole can be attributed largely an effect components on lymphocyte viability. It is hypothesised arises as a result oxidation spermine amine oxidase enzyme present fetal calf serum. In support this hypothesis, it was found cytotoxicity serum dependent inhibited inhibitor hydroxylamine
Patients with chronic fatigue syndrome (CFS) have a broad and variable spectrum of signs symptoms onsets. This report outlines the results single-blind, cross-sectional research project that extensively investigated large cohort 100 CFS patients 82 non fatigued control subjects aim performing case-control evaluation alterations in standard blood parameters urinary amino organic acid excretion profiles. Blood biochemistry full counts were unremarkable fell within normal laboratory ranges....
Lactoferrin isolated from sow milk (about 0-6 mg/ml) was shown to be chromatographically homogeneous, an observation supported by electrophoresis and reaction against monospecific anti-lactoferrin antiserum. Isoelectric focusing showed multiple forms of the protein (i.e.p., 9-3 10-0) converted neuraminidase one form 9-65). Boar seminal plasma contains immunologically identical lactoferrin (0-1 0-5 which binds strongly boar spermatozoa.
A.R.C Unit of Reproductive Physiology and Biochemistry, 307 Huntingdon Road, Cambridge CB3 0JQ (Received 9th October 1974) Rapid cooling the spermatozoa most mammalian species leads to irreversible decrease in motility metabolism (Milovanov, 1934; Chang & Walton, 1940), leakage intracellular proteins enzymes (Mann Lutwak-Mann, 1955), increase proportion differentially stained cells (Easley, Mayer Bogart, 1942) changes cellular cation concentrations (Blackshaw Salisbury, 1957). These effects...
Staphylococcus lugdunensis has emerged as a major cause of community-acquired and nosocomial infections. This bacterium can rapidly adapt to changing environmental conditions survive capitalize on opportunities colonize infect through wound surfaces. It was proposed that S. would have underlying alterations in metabolic homeostasis provide the necessary levels adaptive protection. The aims this project were examine impacts subtle variations growth characteristics, cell size membrane fatty...
Highlights•Virologic responses to NA therapy in chronic hepatitis B patients show significant variability.•Five distinct virologic trajectory classes with clinical, laboratory and demographic features are identified.•Over 20% of exhibit suboptimal virological treatment.•Complete suppression for on current standard antiviral treatment can be slow.•Slow is associated a twofold increased risk fibrosis/cirrhosis.AbstractBackground & AimsThe dynamics HBV viral load (VL) (CHB) nucleos/tide...
Forty-six patients presenting with chronic orofacial muscle pain and eight age- sex-matched control subjects were investigated for the carriage prevalence of, exotoxin production by, coagulase-negative staphylococci (CNS). The selected from an initial group of 41 on basis absence musculoskeletal symptoms. There was a significantly higher multiple four or more strains CNS in than (23 versus 9 isolates/10 subjects). Two 103 isolates none produced toxic shock syndrome toxin 1 (TSST-1),...
ABSTRACT: This report further characterizes the cytotoxic properties of seminal plasma and provides evidence for a role spermine oxidation in generation cytotoxicity. Addition to lymphocyte cultures was found result effect similar that observed upon addition plasma. Furthermore, although is not serum‐free medium, monoamine oxidase sufficient plasma‐associated Analysis 73 individual samples indicated all were cytotoxic, suggesting this an intrinsic property These results support mechanism...
Within 48 hours of fertilization human preovulatory oocytes in vitro, an immunosuppressive factor was detected the culture medium by a rosette inhibition test. Cultures which oocyte unsuccessful were indistinguishable from control medium. Release early pregnancy preimplantation embryo could be clinical significance to establishment after transfer.