A5041304842- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Forensic Toxicology and Drug Analysis
- Alcohol Consumption and Health Effects
- Protein Kinase Regulation and GTPase Signaling
- Poisoning and overdose treatments
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Animal Behavior and Reproduction
- Liver Disease Diagnosis and Treatment
- Drug Transport and Resistance Mechanisms
- Cardiac Arrest and Resuscitation
- Fossil Insects in Amber
- Kawasaki Disease and Coronary Complications
- Hepatitis C virus research
- Lipoproteins and Cardiovascular Health
- Spider Taxonomy and Behavior Studies
- Restraint-Related Deaths
- Anesthesia and Sedative Agents
- Insect and Pesticide Research
- Coronary Artery Anomalies
- Peroxisome Proliferator-Activated Receptors
- Metabolomics and Mass Spectrometry Studies
- Smoking Behavior and Cessation
- Neurotransmitter Receptor Influence on Behavior
- Cholesterol and Lipid Metabolism
National Institute of Health Sciences
2025
Fukuyama University
2025
Nippon Medical School Hospital
2015-2024
Nippon Medical School
1998-2024
University of Hyogo
2014-2024
Aomori University of Health and Welfare
2024
Museum of Nature and Human Activities Hyogo
2014-2023
Hyogo University
2007-2023
Shioda General Hospital
2023
Hakodate Central General Hospital
2007-2019
The hypothesis that calcineurin, the Ca2+/calmodulin-dependent protein phosphatase, contains an autoinhibitory domain was tested using synthetic peptides corresponding to regions of carboxyl-terminus calcineurin. Of several analyzed, one, containing residues I-T-S-F-E-E-A-K-G-L-D-R-I-N-E-R-M-P-P-R-R-D-A-M-P, gave complete inhibition its phosphatase activity. Using [32P]myosin light chain as substrate IC50 about 10 microM obtained with either native assayed in presence Ca2+/calmodulin, or...
Incubation of purified rat brain Ca2+/calmodulin-dependent protein kinase II for 2 min in the presence Ca2+, calmodulin (CaM), Mg2+, and ATP converted from a completely Ca2+-dependent to substantially Ca2+-independent form with little loss total activity. Subsequent addition EGTA autophosphorylation reaction enhanced further which was associated suppression activity value. Protein phosphatase 1 rapidly increased suppressed back control value slowly decreased Kinetic analysis showed that not...
The genus Myrmarachne MacLeay, 1839 (Araneae: Salticidae) is one of the most diversified salticid groups in Southeast Asia, with 23 species previously recorded from Borneo. Based on collections accumulated 2004 to 2014 Lambir Hills National Park, we herein describe six new species: M. amabilis sp. nov., hashimotoi lagarosoma leptosoma salaputium nov. and tintinnabulum In addition, female endoi Yamasaki & Ahmad, 2013 for first time. male-female combination were confirmed by DNA barcoding.
Abstract The site in calcineurin, the Ca2+/calmodulin (CaM)-dependent protein phosphatase, which is phosphorylated by Ca2+/CaM-dependent kinase II (CaM-kinase II) has been identified. Analyses of 32P release from tryptic and cyanogen bromide peptides derived [32P]calcineurin plus direct sequence determination established as -Arg-Val-Phe-Ser(PO4)-Val-Leu-Arg-, conformed to consensus phosphorylation for CaM-kinase (Arg-X-X-Ser/Thr-). This located at C-terminal boundary putative CaM-binding...
Abstract The 63-kDa subunit, but not the 60-kDa of brain calmodulin-dependent cyclic nucleotide phosphodiesterase was phosphorylated in vitro by autophosphorylated form Ca2+/calmodulin-dependent protein kinase II. When calmodulin bound to phosphodiesterase, 1.33 +/- 0.20 mol phosphate incorporated per subunit within 5 min with no significant effect on enzyme activity. Phosphorylation presence low concentrations resulted a phosphorylation stoichiometry 2.11 0.21 and increased about 6-fold...
We evaluated the synergistic effect of zinc supplementation on response to interferon (IFN) therapy in patients with intractable chronic hepatitis C a pilot study using natural IFN‐α or without zinc. No clinical differences were observed between treated IFN alone ( n =40) and polaprezinc (IFN + Zn, =35). All positive for HCV genotype Ib had more than 10 5 copies virus/mL serum. Ten million units was administered daily 4 weeks followed by same dose every other day 20 weeks. In Zn group,...
Objective— Plasma platelet-activating factor (PAF) acetylhydrolase (AH) is an enzyme bound with lipoproteins that degrades not only PAF but also PAF-like oxidized phospholipids are proposed to promote atherosclerosis. In this study, we investigated the distribution of PAF-AH protein among lipoprotein classes by using adenovirus-mediated gene transfer in mice, and examined its effects on oxidation foam cell formation macrophages. Methods Results— Adenovirus-mediated overexpression mice...
Diffuse alveolar damage (DAD) is a pathological hallmark of severe interstitial lung diseases, such as acute respiratory distress syndrome (ARDS), and linked to poor prognosis. Previously, we identified 14–3–3σ/stratifin (SFN) serum biomarker candidate for diagnosing DAD. To clarify the time-dependent relationship between SFN expression DAD, here investigated molecular changes in serum, bronchoalveolar lavage fluid (BALF), tissue an oleic acid (OA)-induced ARDS rat model. Acute edema was...
Abstract The present study explores toxicologic significance of blood cyanide concentrations in fire victims. Headspace gas chromatography was used for detection. Analysis samples from ten victims (postmortem interval = 8 h to 3 5 d) detected zero 11.9 mg/L and a large difference among Carboxyhemoglobin (COHb) saturation the range 24.9 84.2%. To examine effects methemoglobinemia postmortem on victims, an experiment carried out using rabbits as animal model. were sacrificed by intramuscular...
To elucidate intracellular events evoked via this receptor, we examined changes caused by PGF,, stimulation in the phosphotyrosine composition of cellular proteins.The addition to cells quiescent culture rapidly increased levels proteins with Mr values 70,000 (pp70), 85,000 (ppS5), 92,000 (pp92), 100,000 (pplOO), and 125,000 (pp125); latter was immunologically identified as p125 focal adhesion kinase.The PGF,,-induced level Ca2+ ([Ca2+],) elevation, formation inositol phosphates,...
There are large individual variations in the responses of risk factors for coronary heart disease to alcohol consumption. To clarify responsible these variations, we studied relationship between blood pressure, serum lipids, and uric acid genetic polymorphisms dehydrogenase (ADH) 2 aldehyde (ALDH) drinkers.We examined 133 male workers who drank >300 g per week. Information regarding lifestyle habits was obtained by questionnaire. The ADH2 genotype determined PCR subsequent digestion with...
A study was conducted to examine the postmortem stability of cocaine and cocaethylene in rabbit blood tissues, determine whether is produced decomposed human specimens containing endogenous ethanol. Heart blood, liver, brain femoral muscle taken from rabbits 20 min after oral administration mg/kg together with 2 g/kg ethanol were kept at 20-25 degrees C for 5 days. Cocaine concentrations order > liver showed very large intersubject variations time death. degraded rapidly liver. However, 12.0...