The corn planthopper, Peregrinus maidis, is a major pest of agronomically-important crops. maidis has large geographical distribution and transmits Maize mosaic rhabdovirus (MMV) stripe tenuivirus (MSpV). objective this study was to develop effective RNAi methods for P. maidis. Vacuolar-ATPase (V-ATPase) an essential enzyme hydrolysis ATP transport protons out cells thereby maintaining membrane ion balance, it been demonstrated be efficacious target in other insects. In study, two genes...

Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), also known as fall armyworm (FAW), is a destructive and highly polyphagous insect pest of corn. We hypothesize that associated weed species corn may be an alternate host FAW. This study investigated how different with – such Rottboellia cochinchinensis (Lour.) Clayton, Ageratum conyzoides L., Amaranthus spinosus Celosia argentea Commelina benghalensis Corchorus olitorius Ipomoea triloba Eleusine indica (L.) Gaertn., Portulaca...

The corn planthopper, Peregrinus maidis (Ashmead) (Hemiptera: Delphacidae), transmits Maize mosaic rhabdovirus (MMV), an important pathogen of maize and sorghum, in a persistent propagative manner. To better understand the vectorial capacity P. maidis, we determined efficiency MMV acquisition by nymphal adult stages, characterized titer through development. Acquisition efficiency, i.e., proportion insects that acquired virus, was reverse transcriptase polymerase chain reaction (RT-PCR) virus...

Abstract Rhabdovirus glycoproteins (G) serve multifunctional roles in virus entry, assembly, and exit from animal cells. We hypothesize that maize mosaic (MMV) G is required for invasion, infection, spread Peregrinus maidis , the planthopper vector. Using a membrane-based yeast two-hybrid assay, we identified 125 P. proteins physically interacted with MMV G, of which 68% matched known functions endocytosis, vesicle-mediated transport, protein synthesis turnover, nuclear import/export,...

The corn planthopper, Peregrinus maidis Ashmead (Hemiptera:Delphacidae), is a widely distributed insect pest which serves as vector of two phytopathogenic viruses, Maize mosaic virus (MMV) and stripe (MStV). It transmits the viruses in persistent propagative manner. MMV an alphanucleorhabdovirus with negative-sense, single-stranded RNA unsegmented genome. One identified protein that may serve receptor to Syntaxin-18 (PmStx18) belongs SNAREs (soluble N-ethylmaleimide-sensitive factor...

Maize mosaic virus (MMV) is a plant that transmitted by the corn planthopper, Peregrinus maidis Ashmead (Hemiptera: Delphacidae) in persistent and propagative manner. MMV causal agent for disease can cause significant loss yield. Management focused on controlling insect vector population. The molecular mechanisms of transmission are not fully understood. Previously, we identified receptor MMV, P. syntaxin 18 (PmStx18). PmStx18 interacts with viral glycoprotein potentially facilitates...

Fall armyworm (FAW), Spodoptera frugiperda, has been causing significant crop losses and now become the most economically important pest of corn globally. With challenges arising from extensive use synthetic chemicals such as reports that FAW gained resistance to various insecticides, researchers are developing environmentally sound control measures address these issues. An example is application RNA interference in managing populations. a biological process where dsRNA molecules silence...

Fall armyworm (FAW), Spodoptera frugiperda, has been causing significant crop losses and now become the most economically important pest of corn globally. With challenges arising from extensive use synthetic chemicals such as reports that FAW gained resistance to various insecticides, researchers are developing environmentally sound control measures address these issues. An example is application RNA interference in managing populations. a biological process where dsRNA molecules silence...

Rice and corn are one of the most important crops in Philippines. Several insect pests contribute to losses low yield these crops. The fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith), was recently reported cause economic damage corn. Also, this consists two genetically differentiated strains namely, strain rice strain. This study conducted a laboratory conditions evaluate biological parameters FAW on varieties. Newly hatched larvae were fed such as open pollinated variety (OPV),...

Theresistance mechanisms exhibited by the five selected maize varieties against Ostrinia furnacalis [Gueene] or Asian corn borer (ACB) were identified and evaluated in study terms of insects’ percent mortality development. The detached leaf-feeding assay was used experiment laid out Complete Randomized Design (CRD) with 5 treatments replicated three times. Findings among tested ACB, Bt had a high degree antibiosis mechanism. This expected because toxin from variety conferring toxicity to...

ABSTRACT The corn planthopper, Peregrinus maidis , is a widely distributed insect pest which serves as vector of two phytopathogenic viruses, Maize mosaic virus (MMV) and stripe (MStV). It transmits the viruses in persistent propagative manner. MMV an alphanucleorhabdovirus with negative-sense, single-stranded (ss) RNA unsegmented genome. One identified protein that may serve receptor to Syntaxin-18 (PmStx18) belongs SNAREs (soluble N-ethylmaleimide-sensitive factor attachment receptors)....

The use of transgenic crops to combat insect infestation has been proven be an effective and environment-friendly approach. However, insecticidal proteins with a single mode action are prone the development resistance. Researchers now interested in combining acquire multiple modes address that problem. Here, we have performed virtual evaluation fusion protein was silico constructed by vegetative (Vip3Aa) Bacillus thurengensis garlic leaf lectin Allium sativum (ASAL) fl exible peptide linker...

Several species of Bacillus are plant growth-promoting rhizobacteria that can produce the phytohormone Indole-3-acetic acid (IAA) which regulates growth and development, in some species, protects host from pathogen invasion. Previous studies reveal several pathways for IAA biosynthesis various bacterial including genes enzymes take part biosynthetic pathway. In this study, we aim to amplify dhaS , one component indole-3-pyruvic pathway (IPyA) synthesis, conduct 16S rRNA sequence analysis two...

The corn planthopper, Peregrinus maidis, is a widely distributed insect pest which serve as vector of two plant pathogenic viruses, Maize mosaic virus (MMV) and stripe (MStV). It transmits the viruses in persistent, circulative, propagative manner. MMV an alphanucleorhabdovirus with negative-sense, single-stranded (ss) RNA unsegmented genome. Previous work using membrane-based yeast hybrid system on this pathosystem identified potential receptors for MMV. One protein Syntaxin-18 (PmSTX18)...

Host-plant resistance is a fundamental part of integrated pest management through breeding for genes conferring against insect pests. One method that can achieve host plant to insects quantitative trait locus (QTL) mapping analysis. This has advanced significantly recently because the availability modern genomic methods and instrumentation, powerful statistical methods, wide range molecular markers. Single nucleotide polymorphism (SNPs) genotyping by sequence (GBS) resulted in high...

A new report of Stenocranus near pseudopacificus Kirkaldy is documented as an insect pest corn in Kalinga, Luzon, Philippines. As the identification this species was done only again first quarter 2021, work understanding its biology and life history infancy compared to other planthoppers with primary plant host, which may share similar genome organization vectorial capacity transmit phytopathogens. Very little knowledge known regarding biological ecological characteristics. Here, number...