Significance Mammalian cells contain only one glycosyltransferase, OGT, that operates in the nucleus and cytoplasm rather than secretory pathway. OGT is required for cell proliferation, but a basic unanswered question which functions are essential. This challenging to address because has thousands of glycosylation substrates, two different enzymatic activities, large number binding partners. Here, by establishing genetic tools replace endogenous with variants preserve subset its we show low...

O-GlcNAc transferase (OGT) is an essential mammalian enzyme that glycosylates myriad intracellular proteins and cleaves the transcriptional coregulator Host Cell Factor 1 to regulate cell cycle processes. Via these catalytic activities as well noncatalytic protein–protein interactions, OGT maintains homeostasis. OGT’s tetratricopeptide repeat (TPR) domain important in substrate recognition, but there little information on how changing TPR impacts its cellular functions. Here, we investigate...

Evidence-based teaching is a highly complex skill, requiring repeated cycles of deliberate practice and feedback to master. Despite existing well-characterized frameworks for practice-based training in K–12 teacher education, the major principles these have not yet been transferred instructor development higher educational contexts, including graduate assistants (GTAs). We sought determine whether program could help GTAs learn use evidence-based methods their classrooms. implemented weekly...

Due to the changing landscape of K-12 education in United States, particularly issues arising from funding, safety, accessibility, and standardization, frequency at which teachers utilize traditional field trips has diminished over last few decades. Thanks continued advances communicative technology, however, are able offer students virtual (VFTs). Although not a perfect substitute for real life trips, VFTs still take beyond classroom walls help bring history, geography, economics, civics...

Journal Article Familial Fitness: Disability, Adoption, and Family in Modern America Get access America. By Sandra M Sufian. (Chicago: University of Chicago Press, 2022. xiv, 375 pp. Cloth, $105.00. Paper, $35.00.) Sarah Potter Memphis, Tennessee Search for other works by this author on: Oxford Academic Google Scholar American History, Volume 110, Issue 2, September 2023, Pages 377–378, https://doi.org/10.1093/jahist/jaad198 Published: 01 2023

Powerful, facile new ways to create libraries of site-directed mutants are demonstrated. These include: (1) one-pot-PCR, (2) multi-pot-PCR, and (3) split-mix-PCR. One-pot-PCR uses mutant oligonucleotides generate megaprimers in situ, it was used randomly incorporate 28 mutations a gabT gene single reaction. In more difficult cases, multi-pot-PCR can be employed: synthesized individually, then combined mutagenesis PCR. This method 14 out 15 pabB gene. Split-mix-PCR is conceptually novel for...

This study investigated the understanding of a criminal act (i.e., assault) as hate crime well perceptions victim and defendant in such scenarios based on perceived bisexual orientation for participants who either reported being an advocate lesbian, gay, bisexual, transgender (LGBT) community or not. In presented scenario gender were held constant (male/male vs. female/female) sexual was varied each condition (gay lesbian heterosexual) stated heterosexual). Results indicated significant...

This article argues that parents of the post–World War II baby boom chose where to live based primarily on characteristics individual neighborhoods, rather than by making sharp distinctions between urban and suburban space. The scholarship domesticity mass suburbanization usually presumes these two phenomena went hand in as baby-boom sought suburbs’ supposedly unique amenities for nuclear family togetherness. case study black white families Chicago reveals instead diverse postwar described...

Abstract Evidence-based teaching is a highly complex skill, requiring repeated cycles of deliberate practice and feedback to master. Despite existing well characterized frameworks for practice-based training in K-12 teacher education, the major principles these have not yet been transferred instructor development higher educational contexts, including graduate assistants (GTAs). We sought determine whether program could help GTAs learn use evidence-based methods their classrooms. implemented...

This article traces the changing sexual politics of Southern Baptist Convention (SBC) from 1950s through 1980s. It argues that moderates who led denomination in 1950s, 1960s, and early 1970s joined other supporters “sexual containment” during Cold War to develop a theology about salvific power marital sex—and personal, social, national harm created by extramarital sex—which undergirded conservatism denomination's fundamentalist leadership rose analysis reframes our understanding Baptists...

Holly Allen offers a compelling analysis of how widely circulated narratives about diverse figures such as the “forgotten man,” “nagging wife,” and Kibei “troublemaker” shaped ordinary men’s women’s understanding their relationship to economic, political, social upheavals Great Depression World War II. posits that these also help us understand era’s vast growth federal power many structural inequalities inherent in emergent welfare state. By analyzing tandem range civic tropes variety core...

Rachel Rains Winslow provides an ambitious and wide-ranging analysis of the rise international adoption from 1940s to 1970s. She suggests that practice became accepted enduring during these years because it provided appealing solution humanitarian crises abroad. From prospective adoptive parents seeking avoid low supply children long wait times for domestic adoptions relief organizations nongovernmental (Ngos) trying save needy Cold War policy makers portray United States as a prosperous,...

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Abstract O-GlcNAc transferase (OGT), found in the nucleus and cytoplasm of all mammalian cell types, is essential for proliferation. Why OGT required growth not known. performs two enzymatic reactions same active site. In one, it glycosylates thousands different proteins, other, proteolytically cleaves another protein involved gene expression. Deconvoluting OGT’s myriad cellular roles has been challenging because genetic deletion lethal; complementation methods have established. Here, we...

Brian Donovan offers an engaging and insightful analysis of the stereotype gold digger across twentieth-century U.S. history. A refers to a woman who is believed pursue romantic relationships for financial gain. This became, argues, “a misogynistic trope through which everyday people made sense marriage, money, law” (p. 6). traces cultural genealogy diggers at key moments throughout twentieth century. From emergence in 1920s, “allotment Annies” during World War II, model actress Anna Nicole...