A5041814330- Glycosylation and Glycoproteins Research
- Toxin Mechanisms and Immunotoxins
- RNA and protein synthesis mechanisms
- Malaria Research and Control
- Transgenic Plants and Applications
- Bacterial Genetics and Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Microbial Metabolites in Food Biotechnology
- Polyamine Metabolism and Applications
- Algal biology and biofuel production
- Carbohydrate Chemistry and Synthesis
- Insect and Pesticide Research
- Erythrocyte Function and Pathophysiology
- Antimicrobial Peptides and Activities
- Complement system in diseases
- RNA Research and Splicing
- Wastewater Treatment and Nitrogen Removal
- Listeria monocytogenes in Food Safety
- Monoclonal and Polyclonal Antibodies Research
- Mosquito-borne diseases and control
- Nuclear Structure and Function
- Insect Resistance and Genetics
- Bacteriophages and microbial interactions
- Blood groups and transfusion
- Lipid Membrane Structure and Behavior
John Wiley & Sons (United States)
2018
New York University
1987-2015
Columbia University Irving Medical Center
1975-2015
Kaplan (United States)
1991-2015
Office of Diversity and Inclusion
2013
Bayer (United States)
2000
Society for Leukocyte Biology
2000
University Medical Center
1980-1994
NYU Langone Health
1993-1994
DuPont (United States)
1992
Chaperonins are thought to participate in the process of protein folding bacteria and eukaryotic mitochondria chloroplasts. While some chaperonins relatively well characterized, structures mammalian unknown. We have expressed a mitochondrial chaperonin 60 Escherichia coli purified recombinant homogeneity. Structural biochemical analyses this establish single toroidal structure seven subunits, contrast homologous bacterial, fungal, plant 60s, which double comprising two layers identical...
In Escherichia coli, the arginine repressor, product of argR gene, in conjunction with L-arginine controls synthesis enzymes biosynthesis. We describe nucleotide sequence including its control region, and show that formation repressor is autoregulated. The region contains two promoters, one which overlaps operator site and, as other arg genes, consists adjacent palindromic sequences ("ARG boxes"). protein an repressor-beta-galactosidase fusion were purified, amino acid N-terminal end portion...
Despite yearly advances in life‐saving and preventive medicine, as well strategic approaches by governmental social agencies groups, significant disparities remain health, health quality, access to care within the United States. The determinants of these include baseline status, race ethnicity, culture, gender identity expression, socioeconomic region or geography, sexual orientation, age. In order renew commitment medical community address disparities, particularly at school level, we must...
Azotobacter synthesizes an extensive internal membranous nework when grown with air (N 2 ), i.e., under conditions these bacteria fix nitrogen. Very slight quantities of membrane, concentrated mainly about the cell periphery, are formed grows fixed nitrogen, ammonia and amino acids. Compared to cells growing ammonia, utilizing atmospheric nitrogen as sole source smaller in size volume, grow one-third slower, lack detectable poly-β-hydroxybutyrate.
The sin gene of Bacillus subtilis encodes a dual-function regulatory protein, Sin, which is negative as well positive regulator alternate developmental processes that are induced at the end vegetative growth in response to nutrient depletion. Sin has been purified homogeneity by using simple two-step procedure. It was found bind developmentally regulated aprE (alkaline protease) two sites vitro. stronger Sin-binding site (SBS-1) located more than 200 bp upstream from transcription start...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTSG-6, an Arthritis-Associated Hyaluronan Binding Protein, Forms a Stable Complex with the Serum Protein Inter-.alpha.-inhibitorHans-Georg Wisniewski, Wilson H. Burgess, Joel D. Oppenheim, and Jan VilcekCite this: Biochemistry 1994, 33, 23, 7423–7429Publication Date (Print):June 1, 1994Publication History Published online1 May 2002Published inissue 1 June...
Abstract Cultures of human peripheral blood leukocytes (PBL) induced with phytohemagglutinin (PHA) and the phorbol ester 12‐0‐tetradecanoylphorbol 13‐acetate (TPA) produced two types cytotoxic proteins, indistinguishable in vitro assay employing murine L 929 cells as targets. One these proteins had antigenic physicochemical properties lymphotoxin (LT). We have identified other cytotoxin tumor necrosis factor (TNF), mainly on basis cross‐reactivity demonstrated antiserum to TNF, also by its...
Hemozoin, the pigment granule which develops within blood stage food vacuole of malaria parasite Plasmodium falciparum, was biochemically characterized. Hemozoin found to be composed 65% protein, 16% ferriprotoporphyrin-IX (hematin), 6% carbohydrate, and trace amounts lipid nucleic acids. The overwhelming majority protein component is a mixture native denatured human globin non-covalently associated with metalloporphyrin. Immunoelectron microscopy, employing anti-human hemoglobin as probe,...
The diterpene ester 12-O-tetradecanoylphorbol-13-acetate (TPA) and several structurally related compounds were tested for their ability to stimulate interferon (IFN) production in primary cultures of human leukocytes. In Ficoll-Hypaque-purified mononuclear cells, TPA treatment alone induced only low levels IFN, but pretreatment cells caused significant enhancement IFN yields produced with phytohemagglutinin or other T cell mitogens. unprocessed derived from plateletpheresis residues buffy...
The small Ras-related GTP binding and hydrolyzing protein Ran has been implicated in a variety of processes, including cell cycle progression, DNA synthesis, RNA processing, nuclear-cytosolic trafficking both proteins. Like other GTPases, appears to function as switch: Ran-GTP Ran-GDP levels are regulated by guanine nucleotide exchange factors GTPase activating proteins, interact differentially with one or more effectors. One such putative effector, Ran-binding 1 (RanBP1), interacts...
The bacterial component responsible for the induction of transient cold agglutinin syndrome in rabbits after intravenous injection heat-killed Listeria monocytogenes type 4B has been purified and biologically chemically characterized. A immunoglobulin M was prepared from high-titer sera resulting immunization with L. subsequently used to monitor purification its induction. isolated a hot phenol-water extract lyophilized by multiple molecular sieve chromatography. Upon chemical analysis...
A nitrogenase system that remains in the supernatant fluid after centrifuging for 3 hr at 180,000 × g can be extracted from Azotobacter vinelandii by osmotic lysis of bacteria. This preparation is oxygen-labile and appears to similar, though not identical, obtained Clostridium pasteurianum . The particulate characteristic oxygen stability previously described preparations are likely due method cell disruption, e.g., French pressure cell. data support a model intact which enzymes protected...