A5004772008- Advanced Electron Microscopy Techniques and Applications
- Electron and X-Ray Spectroscopy Techniques
- Photosynthetic Processes and Mechanisms
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Advanced X-ray Imaging Techniques
- Machine Learning in Materials Science
Institut de minéralogie, de physique des matériaux et de cosmochimie
2021-2023
Sorbonne Université
2022-2023
Centre National de la Recherche Scientifique
2022-2023
Structure et Instabilité des Génomes
2023
Génomique Bioinformatique et Applications
2022
Single-particle cryo-electron microscopy (cryo-EM) is a technique for biomolecular structure reconstruction from vitrified samples containing many copies of complex (known as single particles) at random unknown 3D orientations and positions. Cryo-EM allows reconstructing multiple conformations the complexes images same sample, which usually requires rounds 2D classifications to disentangle interpret combined conformational, orientational, translational heterogeneity. The elucidation...
Understanding how structure and function meet to drive biological processes is progressively shifting the cryoEM field towards a more advanced analysis of macromolecular flexibility. Thanks techniques such as single-particle electron tomography, it possible image macromolecule in different states, information that can subsequently be extracted through image-processing methods build richer approximation conformational landscape. However, interoperability all these algorithms remains...
Cryo electron microscopy (cryo-EM) instrumentation allows obtaining 3D reconstruction of the structure biomolecular complexes in vitro (purified studied by single particle analysis) and situ (complexes cells cryo tomography). Standard cryo-EM approaches allow high-resolution only a few conformational states molecular complex, as they rely on data classification into given number classes to increase resolution from few, most populated discard all other classes. Such discrete-classification...
Cryo electron microscopy (cryo-EM) allows high-resolution 3D reconstruction of biomolecular structures from highly noisy 2D parallel-beam projection images containing tens thousands copies the same macromolecular complex but at different random orientations and positions. However, complexes are not rigid flexible entities that change their conformations gradually (continuous transition with many intermediate states) to accomplish biological functions (e.g., DNA replication, protein...
Cryo electron microscopy (cryo-EM) instrumentation allows obtaining 3D reconstruction of the structure biomolecular complexes in vitro (purified studied by single particle analysis) and situ (complexes cells cryo tomography). Standard cryo-EM approaches allow high-resolution only a few conformational states molecular complex, as they rely on data classification into given number classes to increase resolution from most populated while discarding all other classes. Such discrete result...