A5054707266- CRISPR and Genetic Engineering
- DNA Repair Mechanisms
- Polyomavirus and related diseases
- Renal and related cancers
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Pluripotent Stem Cells Research
- Lung Cancer Research Studies
- Cancer therapeutics and mechanisms
- Bacterial Genetics and Biotechnology
University of Sheffield
2010-2025
The topoisomerase I (TOP1) inhibitor irinotecan triggers cell death by trapping TOP1 on DNA, generating cytotoxic protein-linked DNA breaks (PDBs). Despite its wide application in a variety of solid tumors, the mechanisms cancer resistance to remains poorly understood. Here, we generated colorectal (CRC) models for and report that is neither due downregulation main cellular target nor upregulation key PDB repair factor TDP1. Instead, faster PDBs underlies resistance, which associated with...
Human pluripotent stem cells (hPSCs) are of fundamental relevance in regenerative medicine. Naïve hPSCs hold promise to overcome some the limitations conventional (primed) hPSCs, including recurrent epigenetic anomalies. Naïve-to-primed transition (capacitation) follows transcriptional dynamics human embryonic epiblast and is necessary for somatic differentiation from naïve hPSCs. We found that capacitated transcriptionally closer postimplantation than This prompted us comprehensively study...
We report a simple and effective means to increase the biosynthetic capacity of host CHO cells. Lonza proprietary CHOK1SV® cells were evolved by serial sub-culture for over 150 generations at 32 °C. During this period specific proliferation rate hypothermic gradually recovered become comparable that routinely maintained 37 Cold-adapted cell populations exhibited (1) significantly increased volume biomass content (exemplified total RNA protein), (2) mitochondrial function, (3) an antioxidant...