A5005204699- Animal testing and alternatives
- Effects and risks of endocrine disrupting chemicals
- Carcinogens and Genotoxicity Assessment
- Inhalation and Respiratory Drug Delivery
- Risk and Safety Analysis
- 3D Printing in Biomedical Research
- Restraint-Related Deaths
- Advanced Chemical Sensor Technologies
- Neonatal Respiratory Health Research
- Asthma and respiratory diseases
- Medical Imaging and Pathology Studies
- Pesticide Exposure and Toxicity
Charles River Laboratories (United Kingdom)
2021-2025
University of Calgary
1991
The aim of the study was to use multiple in vitro assays assess effects a model irritant, sodium dodecyl sulphate (SDS) (≤10 mM (0.29 %, w/v)), on an airway, MucilAir™. MucilAir™ recovery studies also explored. A 24 h exposure increased IL-8 release at SDS concentration ≥0.63 (0.018 w/v). Mucin secretion and transepithelial electrical resistance (TEER) decreased concentrations ≥1.25 (0.04 Cytotoxicity (lactate dehydrogenase (LDH) into basolateral chamber) observed ≥2.5 (0.07 sensitivity >...
This work evaluated a non-animal toolbox to be used within next-generation risk assessment (NGRA) framework assess chemical-induced lung effects using human upper and lower respiratory tract models, namely MucilAir™-HF EpiAlveolar™ systems, respectively. A 12-day substance repeated exposure scheme was established explore potential through analysis of bioactivity readouts from the tissue integrity functionality, cytokine/chemokine secretion, transcriptomics. Eleven benchmark chemicals were...
Introduction: The United States Environmental Protection Agency (US EPA) has issued a directive to reduce mammalian studies 30% by 2025. application of new approach methodologies is being implemented provide information on chemical hazard and risk assessment. In vitro methods, such as the MucilAir™ assay, are used detect airway damage acute irritation potential in agrochemicals. Materials Methods: Four agrochemicals, including Adepidyn™ two developmental chemicals (DC 1 2), were evaluated...
Hydrogen sulfide is an irritant and chemical asphyxiant gas that exerts its primary toxic effects on the respiratory neurological systems. Exposure to hydrogen above a threshold value of 200-300 ppm characterized by sudden onset hemorrhagic pulmonary edema. The purpose this study was determine whether response associated with changes in surface properties surfactant. Bronchoalveolar lavage fluid retrieved from lungs Fischer 344 rats exposed two concentrations or fresh air for 4 h. Surface...
In vivo models (mostly rodents) are currently accepted by regulatory authorities for assessing acute inhalation toxicity. Considerable efforts have been made in recent years to evaluate vitro human airway epithelial (HAEM) as replacements testing. the current work, an organotypic rat model (RAEM), EpiAirway, was developed and characterized allow a direct comparison with available HAEM, order address potential interspecies variability responses harmful agents. The were evaluated 2 independent...