A5012707624- Renal and related cancers
- Genetic and Kidney Cyst Diseases
- Neonatal Respiratory Health Research
- Renal cell carcinoma treatment
- Pancreatic function and diabetes
- Single-cell and spatial transcriptomics
- Muscle Physiology and Disorders
- Tissue Engineering and Regenerative Medicine
- Immunotherapy and Immune Responses
- Cardiovascular Disease and Adiposity
- Monoclonal and Polyclonal Antibodies Research
- Cell Image Analysis Techniques
- Atherosclerosis and Cardiovascular Diseases
- Pancreatic and Hepatic Oncology Research
- Cellular Automata and Applications
- Advanced Materials and Mechanics
- Plant Molecular Biology Research
- Pediatric Urology and Nephrology Studies
- Signaling Pathways in Disease
- T-cell and B-cell Immunology
- S100 Proteins and Annexins
- Congenital heart defects research
- Epigenetics and DNA Methylation
ETH Zurich
2018-2023
SIB Swiss Institute of Bioinformatics
2019-2023
University of Bern
2023
Science for Life Laboratory
2016
Yale University
1988
Branching patterns and regulatory networks differ between branched organs. It has remained unclear whether a common mechanism exists how organ-specific can emerge. Of all previously proposed signalling-based mechanisms, only ligand-receptor-based Turing based on FGF10 SHH quantitatively recapitulates the lung branching patterns. We now show that GDNF-dependent of cultured wildtype mutant ureteric buds, achieves similar when directing domain outgrowth in silico. further predict confirm...
During lung development, epithelial branches expand preferentially in a longitudinal direction. This bias outgrowth has been linked to cell shape and the division plane. How this arises is unknown. Here, we show that biased occurs independent of surrounding mesenchyme, preferential turnover extracellular matrix at bud tips FGF signalling. There also no evidence for actin-rich filopodia tips. Rather, find tubes be collapsed during early kidney observe fluid flow narrow tubes. By simulating...
ABSTRACT Generating comprehensive image maps, while preserving spatial three-dimensional (3D) context, is essential in order to locate and assess quantitatively specific cellular features cell-cell interactions during organ development. Despite recent advances 3D imaging approaches, our current knowledge of the organization distinct cell types embryonic pancreatic tissue still largely based on two-dimensional histological sections. Here, we present a light-sheet fluorescence microscopy...
During kidney development, reciprocal signaling between the epithelium and mesenchyme coordinates nephrogenesis with branching morphogenesis of collecting ducts. The mechanism that positions renal vesicles, thus nephrons, relative to ureteric buds has remained elusive. By combining computational modeling experiments, we show geometric effects concentrate key regulator, WNT9b, at junctions parent daughter branches where vesicles emerge, even when uniformly expressed in epithelium. This...
During kidney development, reciprocal signalling between the epithelium and mesenchyme coordinates nephrogenesis with branching morphogenesis of collecting ducts. The mechanism that positions renal vesicles, thus nephrons, relative to ureteric buds has remained elusive. By combining computational modelling experiments, we show geometric effects concentrate key regulator, WNT9b, at junctions parent daughter branches where vesicles emerge, even when uniformly expressed in epithelium. This...
ABSTRACT During lung development, epithelial branches expand preferentially in longitudinal direction. This bias outgrowth has been linked to a cell shape and the division plane. How this arises is unknown. Here, we show that biased occurs independent of surrounding mesenchyme, preferential turn-over extracellular matrix at bud tips, FGF signalling. There also no evidence for actin-rich filopodia tips. Rather, find tubes be collapsed during early kidney observe fluid flow narrow tubes. By...
Genome-wide association studies (GWAS) identified 56 loci that are robustly associated with the risk of coronary artery disease (CAD). Only a handful genes in these currently known to influence CAD. We previously showed that: 1) CAD-associated exert their effect at least part by influencing atherosclerotic plaque formation; 2) zebrafish larvae promising model system for high-throughput screens early-stage atherosclerosis. now aim identify causal GWAS-identified CAD using system. Heterozygous...
A common developmental process, called branching morphogenesis, generates the epithelial trees in a variety of organs, including lungs, kidneys, and glands. How morphogenesis can create architectures very different shapes functions remains elusive. In this review, we compare its regulation lungs kidneys discuss role signaling pathways, mesenchyme, extracellular matrix, cytoskeleton as potential organ-specific determinants branch position, orientation, shape. Identifying organ shape their...
Abstract Generating comprehensive image maps, while preserving spatial 3D context, is essential to quantitatively assess and locate specific cellular features cell-cell interactions during organ development. Despite the recent advances in imaging approaches, our current knowledge of organization distinct cell types embryonic pancreatic tissue still largely based on 2D histological sections. Here, we present a light-sheet fluorescence microscopy approach pancreas map at key development time...