Chickpea (Cicer arietinum L.) peptides have shown in vitro potential to inhibit the angiotensin I-converting enzyme (ACE-I). However, molecular interactions between chickpea (CP) and ACE-I as well their ADMET (absorption/distribution/metabolism/excretion/toxicity) characteristics remain unknown. Thus, our aim was study silico of CP with characteristics. Legumin provicilin sequences were submitted analysis search for inhibitory peptides. Simulated enzymatic hydrolysis performed using...

Gluten-related disorders are not considered of relevance at public health level in Central America. The prevalence gluten-related disorders, and adherence to a gluten-free diet, remain unknown the American region. We conducted cross-sectional survey population from San Salvador, El estimate rates self-reported diet. 1326 individuals were surveyed. Self-reported (95% Confidence Interval): gluten sensitivity 3.1% (2.3–4.2); physician-diagnosed celiac disease 0.15% (0.04–0.5); wheat...

Background . Celiac disease seems to be rare in Colombians, but there are currently no data about the prevalence rates of symptomatic adverse reactions gluten or adherence gluten-free diet (GFD) this population. Aim evaluate self-reported gluten, GFD, and gluten-related disorders at population level Colombia. Methods A self-administered questionnaire-based cross-sectional study was conducted a from Northwest Results The estimated were (95% CI) 7.9% (6.5–9.6) 5.3% (4.1–6.7) for recurrent...

Studies on antihypertensive chickpea protein hydrolysates have rarely performed in vivo evaluations, limiting the entry of such into functional food development and clinical trials. Thus, our aim was to optimize hydrolysis conditions produce an alcalase-based hydrolysate with a hypotensive effect at convenient oral doses. The reaction time, temperature, alcalase/substrate concentration were optimized using response surface analysis (RSA). ACE-I inhibition variable. time = 0.5 h, temperature...

Background: The prevalence of gluten-related disorders (GRD) and adherence to a gluten-free diet (GFD) remains unknown in Brazilian population there is no published information on the scientific literature about proportion Brazilians that were diagnosed with disorder. Thus, aim this work was estimate GRDs GFD by self-report adult population. Materials Methods: A questionnaire-based cross-sectional study conducted two cities. Results: response rate 93.2% (1630/1749). self-reported rates (95%...

Chickpea hydrolysates could have antihypertensive potential, but there are no evaluations in vivo. Thus, the potential of a chickpea protein hydrolysate obtained before and after extrusion (a process that modifies digestibility) was evaluated. Protein precipitates were from extruded unextruded flours by isoelectric precipitation hydrolyzed (α-amylase/pepsin/pancreatin). Chemical composition determined (standard methods). ACE-I inhibition assays carried out using colorimetric test. For effect...

The characteristics of food allergen labelling are relevant for avoiding accidental exposure to the allergens interest but no Latin American country has evaluated these characteristics. Our aim was evaluate and precautionary (PAL) in six countries. All data were collected directly from supermarkets surveyed. A total 10,254 packaged products analyzed, which 63.3% (n = 6494) 33.2% 3405) featured and/or PAL, respectively. Most complied with local regulations (≥87.4% both locally produced...

After the global challenges posed by COVID-19, researchers strived to identify risk factors for severe cases, which lead various complications - including death. Lifestyle modifications, such as implementing a healthy diet and recommended physical activity, have shown be protective against COVID-19 cases. Despite an association of plant-based with reduced severity, specific dietary characteristics not been identified. Also, methodology measuring activity is variable, studies overlooking...

Gluten-related disorders (GRDs) are increasing around the world, but their magnitude and relevance remain unknown in most Latin American countries. Thus, an online survey was conducted to estimate prevalence of GRDs as well adherence a gluten-free diet (GFD) Paraguayan adult population. There were 1058 individuals surveyed using validated questionnaire (response rate 93.9%). The self-reported rates follows (95% CI): gluten sensitivity (GS), 10.30% (8.53–12.29); non-celiac GS (NCGS), 5.19%...

The prevalence of food allergy (FA) has not been estimated at a population level in Central American countries and, consequently, the magnitude and relevance problem region remains unknown. Thus, our aim was to evaluate parent-reported FA schoolchildren from country El Salvador. A Spanish version structured questionnaire utilized. Five hundred eight (508) parents returned with valid responses (response rate, 32%). rates (95% CI) were: adverse reactions 15.9 (13.0–19.3), “perceived FA, ever”...

Most food allergy cases are associated with a limited group of allergens. This could be attributed to an increased ability some foods sensitize and trigger allergic reactions. However, there no validated animal models evaluate the sensitizing or allergenic potentials proteins. Our aim was three protocols adjuvant-free intraperitoneal sensitization that differ in time points for sample collection (days 14, 28 35 from beginning sensitization) also number immunizations (2, 5 3, respectively)....

Background: The first cases of food allergy to amaranth grain have recently been published. This pseudocereal is considered hypoallergenic, and there scarce information about the allergenic potential proteins, either before or after processing. Objective: To evaluate, in a mouse model allergy, sensitizing extruded non-extruded albumin globulin fractions from grains. Materials Methods: Amaranth (Amaranthus hypochondriacus) flour was obtained isolated. These protein were also extrusion. An...

Chickpea (Cicer arietinum L.) peptides can inhibit dipeptidyl peptidase IV (DPP-IV), an important type 2 diabetes mellitus therapeutic target. The molecular interactions between the inhibitory and active site of DPP-IV have not been thoroughly examined, nor their pharmacokinetic properties. Therefore, predictions legumin- provicilin-derived peptides, with DPP-IV, properties were carried out. Ninety-two unique identified. Papain trypsin enzymes highest AE (0.0927) lowest BE (6.8625 × 10−7)...

After the global challenges posed by COVID-19, researchers strived to identify risk factors for severe cases, which lead various complications—including death. Lifestyle modifications, such as implementing a healthy diet and recommended physical activity, have been shown be protective against COVID-19 cases. Despite an association of plant-based with reduced severity, specific dietary characteristics not identified. Also, methodology measuring activity is variable, studies overlooking...

Background: A Spanish version of a questionnaire intended to estimate, at the population level, prevalence rates self-reported gluten-related disorders and adherence gluten-free diets has been applied in four Latin American countries. However, idiom issues have hampered application Brazilian population. Thus, aim present study was carry out translation, cultural adaptation, evaluation Portuguese estimate Materials Methods: Two bilingual Portuguese–Spanish health professionals carried...

Background and objectives: The epidemiology of food allergy (FA) food-dependent anaphylaxis remains unknown in Colombia. Our aim was to estimate by parent-report the prevalence FA a Colombian population schoolchildren. Materials methods: A printed questionnaire sent parents schoolchildren aged 5–12 years old from Medellín, Colombia order collect FA-related data. Results: Nine hundred sixty-nine (969) returned with valid responses (response rate, 52.5%). estimated rates (95% CI) were: adverse...

Lupin (Lupinus angustifolius L.) proteins are potential sources of bioactive peptides (LBPs) that can inhibit dipeptidyl peptidase IV (DPP-IV) and angiotensin I-converting enzyme (ACE-I) activity. However, the capacity different enzymes to release LBPs, pharmacokinetic bioactivities released, their binding affinities with active sites DPP-IV ECA-I topics scarcely addressed. Therefore, we used in silico hydrolysis (BIOPEP-UWM platform) various predict LBPs. Among identified lupin (n = 4813),...

To determine functional food consumption preferences in Culiacán, Mexico. A survey was designed, validated, and implemented to gather information on consumption. Associations between health conditions were evaluated. Using the Borda count methodology, preference order of different foods identified, seeking statistical differences preferences. validated questionnaire obtained for application. Association found having a diagnosed illness, undergoing medical treatment, sex with awareness foods;...

The magnitude and relevance of food allergies in the preschool population remain unknown most regions Mexico Latin America. Thus, our aim was to estimate parent-reported prevalence a Mexican characterize their clinical diagnosis presentation. A cross-sectional survey conducted Culiacán City. validated questionnaire utilized. total 810 parents responded (valid response rate, 40.7%). estimated rates (95% CI) were: "physician-diagnosed Food Allergy (FA), ever" 5.30% (3.86-7.08); "immediate-type...

BALB/c mice can be orally sensitized to food proteins under acid suppressive medication, mimicking human exposure and triggering a human-like allergic immune response. However, the reproducibility of such an oral allergy model remains questionable. Our aim was evaluate IgE responses triggered against ovalbumin (OVA) cow’s milk (CMP) after intragastric (IG), either gastric-acid suppression or not, intraperitoneal (IP) sensitization in mice. OVA (0.2 mg) different concentrations CMP were...

There are no epidemiological data about food-dependent exercise-induced anaphylaxis (FDEIA) in Latin America. Our aim was to design, assess, and validate a questionnaire identify potential FDEIA cases and/or estimate its prevalence by self-report. Questions were included the instrument address main symptoms of FDEIA, type/intensity physical activity, anaphylaxis. The instrument's clarity, comprehension repeatability evaluated. These evaluations carried out Hispanic people...

Sugar-free (SF), sugar-reduced (SR), or low-glycemic-index (low GI) cereal products could be helpful for the dietary treatment of disorders related to glucose homeostasis. However, access and economic aspects are barriers that hamper their consumption. Thus, availability price such were evaluated in Northwestern México. The categorized 10 groups. data collected five cities by store visitation (from November 2015 April 2016). specialized stores supermarkets was expressed as rates based on...

The search for an animal model to evaluate the allergenic potential of processed food products is still ongoing. Both sensitization ovalbumin (OVA) in different structural states and allergic response triggered after intragastric or challenges were assessed. BALB/c mice sensitized intraperitoneally OVA (50 µg) (native OVA, N-OVA; denatured D-OVA; formaldehyde- lysine-treated FK-OVA; OVA-FK, OVA-DFK; peptides from pepsin digestion, Pep-OVA). Anti-OVA-specific IgE responses evaluated using...