A5044335396- Photosynthetic Processes and Mechanisms
- Coenzyme Q10 studies and effects
- Mitochondrial Function and Pathology
- Biochemical Acid Research Studies
- Vitamin K Research Studies
- Algal biology and biofuel production
- Antioxidant Activity and Oxidative Stress
- Aquatic Ecosystems and Phytoplankton Dynamics
- Water Quality and Resources Studies
- Enzyme Catalysis and Immobilization
- Geochemistry and Geologic Mapping
- Vitamin C and Antioxidants Research
- Innovations in Aquaponics and Hydroponics Systems
- Heavy metals in environment
- Microbial Metabolic Engineering and Bioproduction
- Hormonal Regulation and Hypertension
- Plant Stress Responses and Tolerance
University of Florida
2001-2025
Everglades University
2023
University of Nebraska–Lincoln
2015
Land plants possess the unique capacity to derive benzenoid moiety of vital respiratory cofactor, ubiquinone (coenzyme Q), from phenylpropanoid metabolism via β-oxidation p-coumarate form 4-hydroxybenzoate. Approximately half in comes this pathway; origin rest remains enigmatic. In study, Phe-[Ring-13C6] feeding assays and gene network reconstructions uncovered a connection between biosynthesis that flavonoids Arabidopsis (Arabidopsis thaliana). Quantification tomato (Solanum lycopersicum)...
Mutation of Arabidopsis thaliana NAD(P)H DEHYDROGENASE C1 (NDC1; At5g08740) results in the accumulation demethylphylloquinone, a late biosynthetic intermediate vitamin K1. Gene coexpression and phylogenomics analyses showed that conserved functional associations occur between K biosynthesis NDC1 homologs throughout prokaryotic eukaryotic lineages. Deletion Synechocystis ndbB, which encodes for one such homolog, resulted same defects as those observed cyanobacterial demethylnaphthoquinone...
Flavonols are subclasses of flavonoids, with hundreds structures identified in plants. This chemical diversity primarily arises from glycosylation, where sugars selectively added to the flavonol backbone. While profiles vary across species and organs, evolutionary forces shaping this chemodiversity physiological significance specific glycosides remain a mystery. Here, we reveal that finely tuned transcriptional regulation sugar selectivity glycosyltransferases drive formation distinct organ...
Plants have evolved the ability to derive benzenoid moiety of respiratory cofactor and antioxidant, ubiquinone (coenzyme Q), either from β-oxidative metabolism p-coumarate or peroxidative cleavage kaempferol. Here, isotopic feeding assays, gene co-expression analysis reverse genetics identified Arabidopsis 4-COUMARATE-COA LIGASE 8 (4-CL8; At5g38120) as a contributor β-oxidation for biosynthesis. The enzyme is part same clade (V) acyl-activating enzymes than At4g19010, CoA ligase known play...
Ubiquinone (Coenzyme Q) is a vital respiratory cofactor and liposoluble antioxidant. In plants, it not known how the C-6 hydroxylation of demethoxyubiquinone, penultimate step in ubiquinone biosynthesis, catalyzed. The combination cross-species gene network modeling along with mining embryo-defective mutant databases Arabidopsis thaliana identified embryo lethal locus EMB2421 (At1g24340) as top candidate for missing plant demethoxyubiquinone hydroxylase. marked contrast prototypical...
The proteinogenic branched-chain amino acids (BCAAs) leucine, isoleucine and valine are essential nutrients for mammals. In plants, BCAAs double as alternative energy sources when carbohydrates become limiting, the catabolism of providing electrons to respiratory chain intermediates tricarboxylic acid cycle. Yet, actual architecture degradation pathways is not well understood. this study, gene network modeling in Arabidopsis rice, plant-prokaryote comparative genomics detected candidates...
Knowledge of arsenic background concentrations in urban soils is important for making remediation decisions. The soil cleanup target level (SCTL) Florida lies within the range concentrations. residential SCTL also near practical quantification limits using analytical procedures. Currently no standard protocols are available determining soils, apart from site-specific cases. Therefore, a pilot study was conducted to develop and employ appropriate determine distribution soils. This involved:...
Abstract Prenylated quinones are membrane-associated metabolites that serve as vital electron carriers for respiration and photosynthesis. The UbiE (EC 2.1.1.201)/MenG 2.1.1.163) C-methyltransferases catalyze pivotal ring methylations in the biosynthetic pathways of many these quinones. In a puzzling evolutionary pattern, prokaryotic eukaryotic UbiE/MenG homologs segregate into 2 clades. Clade 1 members occur universally prokaryotes eukaryotes, excluding cyanobacteria, include mitochondrial...
Growing vegetables in controlled environments (CEs), such as hydroponics, aquaponics, and vertical structures, is a rapidly expanding industry Florida the United States, especially nearby urban areas. Although lettuce ( Lactuca sativa ) still mostly produced fields, growing CEs proximal to areas has become increasingly popular because it may facilitate reduced transportation time associated postharvest degradation. Lettuce among top-most consumed States could provide some of nutrition...