A5000522119- SARS-CoV-2 and COVID-19 Research
- Pediatric health and respiratory diseases
- Tryptophan and brain disorders
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Plant Molecular Biology Research
- Gut microbiota and health
- Viral gastroenteritis research and epidemiology
- HIV Research and Treatment
- Cytomegalovirus and herpesvirus research
- Animal Virus Infections Studies
- Plant Parasitism and Resistance
- SARS-CoV-2 detection and testing
- vaccines and immunoinformatics approaches
- Marine Sponges and Natural Products
- Animal Genetics and Reproduction
- Respiratory viral infections research
- Genomics and Phylogenetic Studies
- Epigenetics and DNA Methylation
- Chromosomal and Genetic Variations
- Metabolomics and Mass Spectrometry Studies
- Physiological and biochemical adaptations
- Plant-Microbe Interactions and Immunity
- Reproductive biology and impacts on aquatic species
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sulfur Compounds in Biology
University of Florida
2008-2024
The Arabidopsis thaliana Elongator complex subunit2 (ELP2) genetically interacts with NONEXPRESSOR OF PATHOGENESIS-RELATED GENES1 (NPR1), a key transcription coactivator of plant immunity, and regulates the induction kinetics defense genes. However, mechanistic relationship between ELP2 NPR1 how gene are unclear. Here, we demonstrate that is an epigenetic regulator required for pathogen-induced rapid transcriptome reprogramming. We show functions in transcriptional feed-forward loop...
Cellular thiols such as cysteine spontaneously and readily react with the respiratory intermediate fumarate, resulting in formation of stable S-(2-succino)-adducts. Fumarate-mediated succination increases certain tumors response to glucotoxicity associated diabetes. Therefore, S-(2-succino)-adducts S-(2-succino)cysteine (2SC) are considered oncometabolites biomarkers for human disease. No disposal routes S-(2-succino)-compounds have been reported prior this study. Here, we show that Bacillus...
The lung microbiome impacts on function, making any smoking-induced changes in the potentially significant. complex co-occurrence and co-avoidance patterns between bacterial taxa lower respiratory tract (LRT) were explored for a cohort of active (AS), former (FS) never (NS) smokers. Bronchoalveolar lavages (BALs) collected from 55 volunteer subjects (9 NS, 24 FS 22 AS). LRT composition was assessed using 16S rRNA amplicon sequencing. Identification differentially abundant patterns,...
Abstract The emergence of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) variants concern (VOC) has raised questions regarding vaccine protection against SARS‐CoV‐2 infection, transmission, and ongoing virus evolution. Twenty‐three mildly symptomatic “vaccination breakthrough” infections were identified as early January 2021 in Alachua County, Florida, among individuals fully vaccinated with either the BNT162b2 (Pfizer) or Ad26 (Janssen/J&J) vaccines. genomes successfully...
Abstract Background The eastern mosquitofish ( Gambusia holbrooki ) has the potential to become a bioindicator organism of endocrine disrupting chemicals (EDCs) due its androgen-driven secondary sexual characteristics. However, lack molecular information on G . hinders use as coupled with biomarker data. While traditional gene-by-gene approaches provide insight for development, holistic analysis would more rapid and expansive determination biomarkers. objective this study was develop utilize...
Abstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Delta variant has caused a dramatic resurgence in infections the United Sates, raising questions regarding potential transmissibility among vaccinated individuals. Methods Between October 2020 and July 2021, we sequenced 4439 SARS-CoV-2 full genomes, 23% of all known Alachua County, Florida, including 109 vaccine breakthrough cases. Univariate multivariate regression analyses were conducted to evaluate...
Summary Despite the limitations of genetic bottlenecks, several invasive species appear to thrive in non-native ranges with extremely low levels sequence-based variation. We previously demonstrated differentiation DNA methylation habitat types highly clonal, genetically depauperate Japanese knotweeds using anonymous markers, but functional relevance this variation is unknown. Here, we sequenced full transcriptome combined a reduced representation bisulfite sequencing approach, epigenotyping...
Abstract Despite improvements in antiretroviral therapy, human immunodeficiency virus type 1 (HIV-1)-associated neurocognitive disorders (HAND) remain prevalent subjects undergoing therapy. HAND significantly affects individuals’ quality of life, as well adherence to and, despite the increasing understanding neuropathogenesis, no definitive diagnostic or prognostic marker has been identified. We investigated transcriptomic profiles frontal cortex tissues Simian (SIV)-infected Rhesus macaques...
Abstract Spaceflight has a unique set of abiotic conditions to which plants respond by orchestrating genome-wide alterations their transcriptome. The methods for preserving RNA analysis are well-established and proven over multiple missions, but, investigating the possible epigenetic mechanisms that may contribute transcriptome alteration not well-developed confining limitations International Space Station (ISS). Currently, used isolate genomic DNA perform analyses ideal frozen plants, as...
To investigate a Florida manatee (Trichechus manatus latirostris) mortality event following red tide bloom in Southwest Florida, an RNA sequencing experiment was conducted. Gene expression changes white blood cells were assessed manatees rescued from affected area (n = 4) and control group 7) using sequencing. The genes with the largest fold compared between two groups to identify molecular pathways related cellular disease processes. In total, 591 (false discovery rate <0.05) differentially...
Abstract The emergence of SARS-CoV-2 variants concern (VOC) has raised questions regarding the extent protection currently implemented vaccines. Ten “vaccination breakthrough” infections were identified in Alachua County, Florida, among individuals fully vaccinated with BNT162b2 mRNA vaccine as a result social or household transmission. Eight presented mild symptoms absence infection other common respiratory viruses, confirmed using viral genetic sequencing. genomes successfully generated...
ABSTRACT Background SARS-CoV-2 Delta variant has caused a dramatic resurgence in infections the United Sates, raising questions regarding potential transmissibility among vaccinated individuals. Methods Between October 2020 and July 2021, we sequenced 4,439 full genomes, 23% of all known Alachua County, Florida, including 109 vaccine breakthrough cases. Univariate multivariate regression analyses were conducted to evaluate associations between viral load (VL) level patient characteristics....
Abstract Background. Despite improvements in antiretroviral therapy, human immunodeficiency virus type 1 (HIV-1)-associated neurocognitive disorders (HAND) remain prevalent subjects undergoing therapy. HAND significantly affects individuals’ quality of life, as well adherence to and, despite the increasing understanding neuropathogenesis, no definitive diagnostic or prognostic marker has been identified.Results. We investigated transcriptomic profiles frontal cortex tissues Simian...
Abstract Background Despite improvements in antiretroviral therapy, human immunodeficiency virus type 1 (HIV-1)-associated neurocognitive disorders (HAND) remain prevalent subjects undergoing therapy. HAND significantly affects individuals’ quality of life, as well adherence to and, despite the increasing understanding neuropathogenesis, no definitive diagnostic or prognostic marker has been identified. Results We investigated transcriptomic profiles frontal cortex tissues Simian...
The lung microbiome impacts function, making any smoking-induced changes in the potentially significant. complex co-occurrence and co-avoidance patterns between bacterial taxa LRT were explored for a cohort of active (AS), former (FS), never (NS) smokers. Bronchoalveolar lavages (BAL) collected from 55 volunteer subjects (nine NS, 24 FS, 22 AS). composition was assessed using 16S rRNA amplicon sequencing. Identification differentially abundant taxa, patterns, discriminant analysis, biomarker...
The lung microbiome impacts function, making any smoking-induced changes in the potentially significant. complex co-occurrence and co-avoidance patterns between bacterial taxa LRT were explored for a cohort of active (AS), former (FS), never (NS) smokers. Bronchoalveolar lavages (BAL) collected from 55 volunteer subjects (nine NS, 24 FS, 22 AS). composition was assessed using 16S rRNA amplicon sequencing. Identification differentially abundant taxa, patterns, discriminant analysis, biomarker...