A5066363677- Photosynthetic Processes and Mechanisms
- Spectroscopy and Quantum Chemical Studies
- Photoreceptor and optogenetics research
- Light effects on plants
- Algal biology and biofuel production
- Photochemistry and Electron Transfer Studies
- Cell Adhesion Molecules Research
- Protein Interaction Studies and Fluorescence Analysis
- Protein Structure and Dynamics
- Porphyrin and Phthalocyanine Chemistry
- Blood properties and coagulation
- Free Radicals and Antioxidants
- RNA Interference and Gene Delivery
- Pharmaceutical and Antibiotic Environmental Impacts
- Proteoglycans and glycosaminoglycans research
- Hemoglobin structure and function
- Quantum Dots Synthesis And Properties
- Molecular spectroscopy and chirality
- Protease and Inhibitor Mechanisms
- Microbial Community Ecology and Physiology
- Metalloenzymes and iron-sulfur proteins
- Barrier Structure and Function Studies
- Erythrocyte Function and Pathophysiology
- ATP Synthase and ATPases Research
- Salivary Gland Disorders and Functions
Lomonosov Moscow State University
2014-2024
Moscow State University
2004-2024
Alberta Children's Hospital
2022
St George's, University of London
1979-1988
St George's Hospital
1979-1988
University of Surrey
1986
University of London
1985
Hungarian Academy of Sciences
1982
HUN-REN Szegedi Biológiai Kutatóközpont
1982
Offshore Renewable Energy Catapult
1980
The adhesion and migration of human diploid fibroblasts on plasma clots were measured. role fibronectin was examined by depleting before clotting. Fibronectin not essential for cell spreading, although rates slightly slower depleted clots. Rates the surface unaffected depletion. In contrast, an absolute requirement cells into Cells migrated rapidly control but completely failed to penetrate fibronectin-depleted effect depletion could only be reversed adding Adsorption after clotting reverse...
Proteoglycan was isolated from cartilage and freed contaminating glycoproteins hyaluronic acid. The macromolecule consists of a protein core covalently linked to number glycosaminoglycan side chains, namely chondroitin sulphate keratan sulphate. This proteoglycan retards the attachment variety cell types tissue culture plastic collagen. Glycosaminoglycans alone, have no significant effect on rates attachment. Similarly, trypsinized is without effect. It concluded that structural integrity...
Sephacryl S-300 gel filtration of animal sera is used to demonstrate that there are at least 2 components promote the 'spreading' cells in culture. A morphometric technique described quantitate spreading process. For a number cell strains and established lines more quantitatively significant factor not fibronectin. Rather it component with fibronectin seems cause via different mechanism from stimulated by Thus will absence protein synthesis, whereas smaller requires synthesis. The kinetics...
ABSTRACT A morphometric assay has been used to show that speed of cell spreading is sensitive the level serum in surrounding growth medium. There was an inverse relationship between concentration and spreading; as increased from 0·1% 100% cells took progressively longer become fully spread. The time taken achieve complete concentrations 0·1, l·0, 10 45, 60, 105 240 min, respectively. At 3% above, fibronectin depletion had no effect on rates spreading. Only at below there although became...
Synovial fluid from rabbit elbow, shoulder, hip and knee was analysed to establish the normal levels of parameters relevant exchange ‐ viz. mass per joint, hydraulic pressure, temperature, colloid osmotic pressure (COP), protein, albumin glycosaminoglycan (GAG) concentrations. Fluid greatest in least congruous shoulder (43 ± 4 mg), cf. 6 2 mg highly hip. In (24 mg) mean thickness layer calculated be 30 µm. subatmospheric all joints (mean ‐2·8 0·4 cmH O, ‐5·7 0·3 0, knee), as many other...
A foetal-calf-serum component has been isolated that stimulates cells in culture to adopt typical fibroblast morphology. This "spreading" factor is a glycoprotein with mol. wt. of approx. 62000 and contains 12% carbohydrate; it an isoelectric point 4.8. The method described allows purification homogeneity as judged by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis analytical focusing. Under cell-culture conditions the active at 18 micrograms/ml.
The composition of afferent lymph draining into the canine popliteal node was compared with that efferent leaving node. Both protein and cellular were studied. In twenty‐five greyhounds concentration greater than collected from same limb. Although absolute level varied greatly between dogs, in a particular animal there constant ratio content lymph. approximately double Chromatographic analysis use radio‐iodinated albumin indicated reason for increased is concentrates Afferent contained less...
Human diploid fibroblasts were seeded onto or into plasma clots and different aspects of cell adhesion migration measured. The roles plasminogen activators plasmin studied by either the removal from prior to clotting addition 10 mM epsilon-aminocaproic acid, which brings about an inhibition in this system. When cells surface clots, rates attachment, spreading, unaffected depletion inhibition. In contrast, when then, although spreading unaffected, was abolished rate measured, there absolute...
Picosecond absorption spectroscopy was used to monitor laser‐induced oxidation‐reductions of reaction center (RC) bacteriochlorophyll (P) and bacteriopheophytin (I) in Rhodopseudomonas sphaeroides RC preparations on exposure different chemicals. The D 2 O isotope substitution H or partial water by organic solvents (ethylene glycol, glycerol, propylene dimethyl sulfoxide) causes the appearance a fast, nanosecond component P + reduction, result an increased probability recombination primary...
The typical elongated bipolar morphology of baby hamster kidney (BHK) cells is not shown by polyoma-transformed BHK (Py-BHK) cells. Instead, the transformed line adheres poorly to tissue-culture plastic and have a more rounded than parent line. Plasma fibronectin known mediate spreading cells, but when human serum subjected Sephacryl S-300 chromatography two peaks activity are eluted; first second, which quantitatively significant, 70 K protein that related stimulates different mechanism....