A5084250577- Neuroscience and Neuropharmacology Research
- Zebrafish Biomedical Research Applications
- Neural dynamics and brain function
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neural Engineering
- Ion channel regulation and function
- Genetics and Neurodevelopmental Disorders
- Photoreceptor and optogenetics research
- Congenital heart defects research
- Developmental Biology and Gene Regulation
- Mitochondrial Function and Pathology
- Neuroscience of respiration and sleep
- RNA modifications and cancer
- Neuroendocrine regulation and behavior
- Neurobiology and Insect Physiology Research
- Sleep and Wakefulness Research
- Receptor Mechanisms and Signaling
- Olfactory and Sensory Function Studies
- Cannabis and Cannabinoid Research
- Ubiquitin and proteasome pathways
- Autism Spectrum Disorder Research
- stochastic dynamics and bifurcation
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Memory and Neural Computing
- Nerve injury and regeneration
Emory University
2015-2025
Georgia Institute of Technology
2024
Wright State University
2024
RELX Group (United Kingdom)
2007
Singer (United States)
2007
National Institutes of Health
1997-2005
National Institute of Neurological Disorders and Stroke
1996-2005
Novartis (Switzerland)
2001
University of Pittsburgh
1993-1995
Roswell Park Comprehensive Cancer Center
1984
DURING BRAIN DEVELOPMENT, THERE IS A PROGRESSIVE REDUCTION OF INTRACELLULAR CHLORIDE ASSOCIATED WITH SHIFT IN GABA POLARITY: depolarizes and occasionally excites immature neurons, subsequently hyperpolarizing them at later stages of development. This sequence, which has been observed in a wide range animal species, brain structures preparations, is thought to play an important role activity-dependent formation modulation functional circuits. sequence also considerably reinforced recently...
Abstract Homeostatic plasticity encompasses a set of mechanisms that are thought to stabilize firing rates in neural circuits. The most widely studied form homeostatic is upward synaptic scaling (upscaling), characterized by multiplicative increase the strength excitatory inputs neuron as compensatory response chronic reductions rate. While reduced spiking trigger upscaling, an alternative possibility glutamatergic transmission generates this directly. However, and neurotransmission tightly...
The acute-phase reactant rabbit serum amyloid A 3 (SAA3) was identified as the major difference product in Ag-induced arthritis rabbit, a model resembling many aspects clinical characteristics of rheumatoid (RA) humans. In arthritis, up-regulated SAA3 transcription vivo detected cells infiltrating into inflamed joint, area where pannus formation starts and, most notably, also chondrocytes. proinflammatory cytokine IL-1beta induced primary chondrocytes vitro. Furthermore, rSAA3 protein matrix...
Many developing networks exhibit a transient period of spontaneous activity that is believed to be important developmentally. Here we investigate the initiation episodes rhythmic in embryonic chick spinal cord. These recur regularly and are separated by quiescent intervals many minutes. We examined role motoneurons their intraspinal synaptic targets (R-interneurons) these episodes. During latter part inter-episode interval, recorded spontaneous, ventral root depolarizations were accompanied...
Cortical hyperexcitability is a hallmark of fragile X syndrome (FXS). In the Fmr1 knockout (KO) mouse model FXS, cortical linked to sensory hypersensitivity and seizure susceptibility. It remains unclear why homeostatic mechanisms fail prevent such activity. Homeostatic intrinsic plasticity (HIP) adjusts membrane excitability through regulation ion channels maintain activity levels following perturbation. Despite critical role HIP in maturation excitability, it has not been examined FXS....
We examined the effects of spontaneous or evoked episodes rhythmic activity on synaptic transmission in several spinal pathways embryonic day 9–12 chick embryos. compared amplitude potentials by stimulation ventrolateral funiculus (VLF), dorsal ventral roots, before and after activity. With exception short-latency responses root stimulation, were briefly potentiated then reduced for minutes an episode Their progressively recovered interval between successive episodes. The lack post-episode...
During early development, gamma-aminobutyric acid (GABA) depolarizes and excites neurons, contrary to its typical function in the mature nervous system. As a result, developing networks are hyperexcitable experience spontaneous network activity that is important for several aspects of development. GABA depolarizing because chloride accumulated beyond passive distribution these cells. Identifying all transporters accumulate immature neurons has been elusive it unknown whether levels different...
Neurons can respond to decreased network activity with a homeostatic increase in the amplitudes of miniature EPSCs (mEPSCs). The prevailing view is that mEPSC are uniformly multiplied by single factor, termed "synaptic scaling." Deviations from purely multiplicative scaling have been attributed biological differences, or distortion imposed detection threshold limit. Here, we demonstrate neurons dissociated cortices male and female mice shift observed experimental data cannot be reproduced...
Mouse models of Fragile X Syndrome (FXS) have demonstrated impairments in sensory-evoked neuronal firing excitatory and inhibitory neurons. Homeostatic plasticity does not compensate for these changes activity. Previous work has shown that homeostatic mechanisms are observed FXS, including deficits synaptic scaling intrinsic excitability. Here, we aimed to examine how sensory integration vivo following a perturbation, unilateral whisker deprivation (WD), an Fmr1 knock out (KO) mouse model....
Studies on the development of synaptic specificity, embryonic activity, and neuronal specification in spinal cord have all been limited by absence a functionally identified interneuron class (defined its unique set connections). Here, we identify an population chick that appears to be avian equivalent mammalian Renshaw cell (R-interneurons). These cells receive monosynaptic nicotinic, cholinergic input from motoneuron recurrent collaterals. They make predominately GABAergic connections back...
A number of homeodomain transcription factors have been implicated in controlling the differentiation various types neurons including spinal motoneurons. Some these proteins are also expressed interneurons, but their function is unknown. Progress understanding role interneuronal development has slow because synaptic connections which part define identity, difficult to establish. Using whole cell recording isolated cord chick embryos, we assessed lumbosacral interneurons expressing...
When neuronal activity is reduced over a period of days, compensatory changes in synaptic strength and/or cellular excitability are triggered, which thought to act manner homeostatically recover normal levels. The time course homeostatic and occur not clear. Although many studies show that 1-2 days block necessary trigger increases excitatory quantal strength, few have been able examine whether these mechanisms actually underlie recovery network activity. Here, we the underlying embryonic...
When activity levels are altered over days, a network of cells is capable recognizing this perturbation and triggering several distinct compensatory changes that should help to recover maintain the original homeostatically. One feature commonly observed after blockade has been increase in excitatory quantal amplitude. The sensing machinery detects central focus field currently, but thus far it elusive. vast majority studies reduce also neurotransmission. We address possibility reduced...
Homeostatic plasticity mechanisms maintain cellular or network spiking activity within a physiologically functional range through compensatory changes in synaptic strength intrinsic excitability. Synaptic scaling is one form of homeostatic that triggered after blockade neurotransmission which the strengths all inputs to cell are multiplicatively scaled upward downward fashion. We have shown previously upscaling could be chick embryo spinal motoneurons by complete GABA<sub>A</sub> receptor...
Using a novel method for retrogradely labeling specific neuronal populations, we tested different styryl dyes in attempt to find whose staining would be specific, rapid, and lead large activity dependent signals. The were injected into the ventral roots of isolated chick spinal cord from embryos at days E9–E12. voltage-sensitive dye signals recorded synaptically activated motoneurons using 464 element photodiode array. best optical obtained relatively hydrophobic di-8-ANEPPQ di-12-ANEPEQ....