The aim of the present study was to compare anchorage TiO 2 ‐blasted screw and cylindrical implants with conventionally used machine‐produced inserted immediately in extraction sockets on dogs. 6 adult mongrel dogs had 3rd 4th mandibular premolars extracted bilaterally 24 commercial pure titanium were placed covered mucoperiosteum. Each dog 4 implants: 1 implant blasted titanium‐dioxide‐particles; (m.p.) surface (controls). After a healing period 12 weeks, 16 from animals for removal torque...

For further characterization of the hybridization properties peptide nucleic acids (PNAs), thermodynamics mixed sequence PNA−DNA duplexes have been studied. We characterized binding PNA to DNA in terms affinity (perfectly matched duplexes) and specificity (singly mismatched using mainly absorption hypochromicity melting curves isothermal titration calorimetry. perfectly sequence-matched varying lengths (6−20 bp), average free energy (ΔG°) was determined be −6.5 ± 0.3 kJ mol-1 bp-1,...

Norfloxacin, a quinolone antibacterial reagent, has been studied with respect to its binding calf thymus DNA using fluorescence and linear dichroism techniques unwinding of supercoiled DNA. The norfloxacin is strongly quenched in the presence this decrease titration equilibrium constant complex formation was established be 2.8 × 103 M-1. electric transition moments chromophore have analyzed anisotropy, magnetic circular dichroism, stretched poly(vinyl alcohol) film INDO/S calculations. These...

The absorption spectra and transition moment directions of the four DNA bases uracil were calculated, utilizing semiempirical INDO/S-CI method ab initio CIS/6-31G* method. A linear scaling energies was necessary to get good agreement with observed energies. CASPT2 π → π* polarizations adenine also calculated. Fully MP2/6-31G*-optimized geometries used in calculations electronic spectra. effect possible tautomerism investigated by calculation spectrum several tautomers guanine cytosine....

A high-throughput pKa screening method based on pressure-assisted capillary electrophoresis (CE) and mass spectrometry (MS) is presented. Effects of buffer type ionic strength sensitivity values were investigated. Influence dimethyl sulfoxide (DMSO) concentration present in the sample effective mobility measurement was examined. series ten volatile buffers, covering a pH range from 2.5 to 10.5 with same strength, employed. The application background electrolytes resulted significant signal...

Solubility and solid-state characteristics were determined multivariate data analysis was used to deduce structural features important for limited solubility of marketed drugs. Molecules with extended ring structures large conjugated systems less soluble, indicating that related rigidity aromaticity result in restricted by stable crystal structures. These descriptors successfully predicted the applied test set can be useful avoiding synthesis compounds behaving like "brick dust".

Current advances of new technologies with robotic automated assays combined highly selective and sensitive LC-MS enable high-speed screening lead series libraries in many vitro assays. In this review, we summarize state the art high throughput for key physicochemical properties such as solubility, lipophilicity, pKa, drug-plasma protein binding brain tissue well ADME profiling. We discuss two primary approaches i.e. an 96-well plate assay integrated a rapid multi-wavelength UV reader....

The electronic spectrum of the unnatural nucleic acid base 2-aminopurine is important in order to understand spectroscopy and photophysics normal DNA bases. Knowledge transition moment directions effective absorption components also a prerequisite for interpretation emission spectra incorporated into as probe motion or excitation energy transfer processes. Using linear dichroism, fluorescence anisotropy, ordinary magnetic circular dichroism techniques, near-UV resolved contributions from...

The thermodynamics of hybridization and the conformations decameric mixed purine-pyrimidine sequence PNA/PNA, PNA/DNA, DNA/DNA duplexes have been studied using fluorescence energy transfer (FET), absorption hypochromicity (ABS), isothermal titration calorimetry (ITC), circular dichroism (CD) techniques. interchromophoric distances determined in FET experiments on fluorescein- rhodamine-labeled indicate that solution structures are extended helices agreement with available NMR (PNA/DNA)...

In order to assess the association between atopy and cancer risk, a cohort of 6593 skin‐prick‐tested patients was established. Among atopic subjects, no overall increased risk found, but incidence both breast (standardized ratio (SIR) 2.50, 95% CI 1.01–5.16) malignant lymphomas (SIR 4.40, 1.20–11.3) significantly enhanced. Atopic subjects with asthma showed decreased 0.73, 95%, 0.27–1.60), as compared other asthmatic 1.46, 1.03–2.04). The for rhinitis near unity 1.11), irrespective whether...

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTA Highly Fluorescent DNA Base Analogue that Forms Watson−Crick Pairs with GuanineL. Marcus Wilhelmsson, Anders Holmén, Per Lincoln, Peter E. Nielsen, and Bengt NordénView Author Information Department of Physical Chemistry Chalmers University Technology S-41296 Gothenburg, Sweden Cite this: J. Am. Chem. Soc. 2001, 123, 10, 2434–2435Publication Date (Web):February 16, 2001Publication History Received14 July 2000Published online16 February...

The electronic spectra of a series adenine derivatives have been investigated with respect to the number transitions, their intensities, and transition moments. experimental work includes linear dichroism (LD) measurements on samples partially oriented in stretched polymer poly(vinyl alcohol) films, fluorescence anisotropy (FA), magnetic circular (MCD). UV both 7-methyladenine (7MA) 9-methyladenine (9MA) are resolved into contributions from five π → π* transitions (I−V). Their polarizations...

Biomarkers of brain damage and head injury are potentially useful tools in the management afflicted patients. Particularly S100B has received much attention been adapted into clinical guidelines. Alcohol intoxication higher age (65 years over) have used as risk factors for serious complications following injury. The effect these on levels not fully established a relevant patient cohort.We prospectively included 621 adult patients with mild traumatic (TBI) sampling. Mild TBI was defined...

The novel fluorescent DNA cytosine base analogue 1,3-diaza-2-oxophenothiazine, tC, has previously been shown to have a remarkably preserved high quantum yield upon incorporation into single strand of peptide nucleic acid, PNA, as well when the latter is hybridized with complementary form PNA−DNA duplex. Here, we investigate fundamental photophysical properties tC. Using fluorescence anisotropy, stretched film linear dichroism, and chemical calculations, transition moment polarizations lowest...

The aim of this study was to evaluate the influence an oral health education program (OHEP) on attitudes among responsible nursing personnel toward performing procedures for care receivers. A total 2882 were offered participation in OHEP, and effect evaluated by means a questionnaire distributed pre‐educationally as well 1–2 months post‐educationally. allocated, basis education, either “high level group (HHCE), including registered enrolled nurses, or a”low education“group (LHCE), assistants...

Reproducibility of the skin prick method testing for allergy was studied in 20 subjects examined by four nurses. Hypodermic needles were used pricking and test panel included a histamine control, diluent nine allergens. The reproducibility best when size weal reaction caused an allergen expressed as geometric area weal. When ratio to that histamine, decreased considerably. ratios further classified into three class ratings, very low. reduction due low reactions. According these results, at...

The chemical reactivity of electrophilic metabolites usually prevents their detection in vivo since, by definition, they are relatively short-lived and likely to undergo one or more structural modifications form stable final products. Electrochemical oxidation provides a means generate reactive an environment without the presence such nucleophiles. This paper describes results our MS, MS/MS, NMR, IR, computational studies on products (and conjugates) that have been generated...

In CNS drug discovery, knowledge of drug-tissue binding is essential for a better understanding brain penetration by assessing unbound to plasma ratio as well pharmacokinetics (PK) and pharmacodynamics (PD) relationship relating free concentration pharmacological effect in target tissues. this work, we present novel microemulsion based capillary electrophoresis (CE) method that enables coupling electrokinetic chromatography (MEEKC) mass spectrometry (MS) prediction biopartitioning drugs...

Smoking and the use of smokeless tobacco have a detrimental impact on general oral health. The relationship to dental caries is however still unclear. As multi-factorial disease with clear life-style, socio-economic socio-demographic gradients, may be co-variable in this complex rather than direct etiological factor. Our aim was analyze incidence among adolescents, consideration variables by residency, using epidemiological data from longitudinal study region Halland, Sweden.The population...