The glycoinositol phospholipid membrane anchor of human erythrocyte acetylcholinesterase (EC 3.1.1.7) contains a novel inositol which in this and the accompanying paper (Roberts, W.L., Santikarn, S., Reinhold, V.N., Rosenberry, T.L. (1988) J. Biol. Chem 263, 18776-18784) is shown to be plasmanylinositol that palmitoylated on ring. was radiolabeled with photoactivated reagent 3-(trifluoromethyl)-3-(m-[125I] iodophenyl)diazirine characterized by various chemical enzymatic cleavage procedures...

Plasma or serum [0.1–1.0 ml] was digested with phospholipase C and total lipid extracts were prepared silylated in the presence of tridecanoylglycerol as internal standard. The neutral free fatty acid profiles determined by means an automated GLC system equipped unheated on-column inlet, time actuated liquid injector, programmed heating, cooling equilibration cycles, electronic peak area integrator. separations accomplished on a 50 cm × 2 mm i.d. steel column packed 3% OV-1 100–120 mesh Gas...

High density lipoprotein (HDL) is rich in polyunsaturated phospholipids that are sensitive to oxidation. However, the effect of apolipoprotein A-I and paraoxonase-1 (PON-1) on phosphatidylcholine oxidation products has not been identified. We subjected native HDL, trypsinized HDL lipid suspensions by peroxynitrite donor, 3-morpholinosydnonimine. had a basal level mono- di-hydroperoxides increased greater extent compared with either or alone. Phosphatidylcholine core aldehydes, which were...

We have compared the molecular species composition of glycerolipids rat liver and plasma very low density lipoproteins (VLDL). There were differences in stereospecific distribution fatty acids triacylglycerols (TG) VLDL. While chiral reversed phase chromatography with mass spectrometry (LC/MS) revealed great similarities positional association between sn-1,2-diacylglycerol (DG) moieties VLDL TG, corresponding sn-2,3-DG distinctly different. The free hepatic sn-1,2-DG moiety contained within...

We have previously shown that Helicobacter pylori specifically binds to a glycerolipid species preferentially found in the antrum of human stomach. now show by high-pressure liquid chromatographic analysis this is form phosphatidylethanolamine and H. bona fide as detected thin-layer chromatogram overlay procedure. Considerable variation binding from different sources was observed, however, suggesting importance nature long-chain hydrophobic moiety. A similar specificity exoenzyme S...

A method of simultaneous determination stereospecific distribution and molecular association acyl groups in triacylglycerols has been developed. The analysis is based on a random generation rac-1, 2-diacylglycerols by Grignard degradation, synthesis rac-phosphatidylcholines, stepwise release 1, 2-sn- 2, 3-sn-diacylglycerols phospholipase C. exact structure the original reconstituted basis complete species as tertiary-butyldimethylsilyl ethers gas chromatography with mass spectrometry....

In order to distinguish between possible fatty acid differences during lumenal lipolysis and cellular absorption, we have reinvestigated the in vitro hydrolysis of menhaden oil its alkyl esters by pancreatic lipase. For this purpose incubated or methyl ethyl with porcine lipase presence bile salts determined composition released free acids, monoacylglycerols, diacylglycerols, residual triacylglycerols, acids esters, respectively, thin-layer gas-liquid chromatography. There was significant...

Abstract This study reports the application of modern methods molecular species analysis in determination structure both major and minor glycerophospholipids sphingomyelins human erythrocytes. Individual phospholipid classes were resolved from total lipid extracts by thin‐layer chromatography. Diradylglycerols released phospholipase C converted into trimethylsilyl ethers, which alkenylacyl, alkylacyl diacylglycerol subclasses normal phase high performance liquid Molecular diradylglycerols...

Abstract In addition to diacyl glycerophosphocholine and sphingomyelin, human plasma also contains small amounts of other glycerophospholipids, which may have special metabolic function. The structure origin these minor lipids has not been determined. Knowledge the detailed composition phospholipids red blood cells (Myher et al., Lipids 24 , 1989) permits evaluation one possible sources. This study reports analyses glycerophospholipids made in parallel those erythrocyte obtained from same...

Abstract The effects of biliary lecithin on fat absorption were studied in 1 day bile fistula rats fed micellar solutions salt, monoglyceride and radioactive free fatty acids. By electron microscopy measurement uptake radioactivity into liver adipose tissue, it was shown that the absence there significant impairment release from mucosa. Fat clearance effected by feeding phosphatidyl choline or choline, but not ethanolamine, inositol cholesterol. In luminal a decrease incorporation leucine...

The development of hyperphospholipidemia and hypercholesterolemia was studied in infants that required total parenteral nutrition given a continuous infusion Intralipid, (1-4 g/kg body wt per 24 h. Detailed studies were carried out on periods lasting 1-10 d. After h there marked increase plasma free cholesterol (68%) phospholipid (77%) concentrations. Based the amount rate infusion, it estimated at least 50% increment during 64-h infusions derived from endogenous sources. By contrast, could...

The triglyceride compositions of the milk fats man, dog, guinea pig, cow, sheep, goat, and horse were compared by gas-liquid chromatography intact triglycerides butyl esters component fatty acids.The which largely made up long-chain acids, showed a common pattern with major contributions glycerides 48-54 acyl carbon atoms.The rich in short-chain significant proportions 28-54 atoms.Horse milk, contains large amounts medium-chain gave characteristic 26-54 atoms range.The experimentally...

Triglycerides with a total of 34-44 acyl carbon atoms have previously been shown to account for nearly 50% bovine milk fat. About 80% this material was isolated by molecular distillation and representative fraction these glycerides subjected stereospecific analysis. Of the 29 moles % C4-C8 fatty acids present in analyzed fraction, at least 95% were specifically attached glycerol molecule position corresponding 3 sn-glycerol 3-(dihydrogen phosphate) (L-α-glycerophosphate). The distribution...

Abstract The stereochemical course of hydrolysis radioactive triolein was determined using lipoprotein lipase cow's milk and rat postheparin plasma. [3H]Glycerol trioleate or glycerol [1-14C]trioleate emulsified with total egg yolk lipids to provide 0.2 2.5 µCi 3H 0.03 14C per µm substrate. products were isolated at 1 45 min free fatty acids the various positional isomers mono- diglycerides resolved by thin layer chromatography. In a yield 0.16 µmole diglyceride, 9 30% 1,3- 70 91%,...

The incorporation of orthopho~phate-~~P and glycerol-2J4C into the various species rat liver phosphatidylinositols as a function time was determined in vivo.3zP administered intraperitoneally glycerol-14C given intravenously.The were resolved intact according to degree unsaturation.1-3 hr after injection labeled phosphate, relative specific activity linoleoyl dienes exceeded that arachidonoyl tetraenes about 17-fold, trienes polyenes 8fold.The activities all fractions became equal 2-3 days...

The specific activities of individual molecular species rat liver diacylglycerylphosphorocholine (PC), diacylglycerylphosphoroethanolamine (PE), and diacylglycerophosphorylinositol (MPI) were determined compared following intravenous injection glycerol- 14 C. PC, PE, MPI contained 41, 51, 83%, respectively, tetraenoic species, 40,17, 9% combined mono-, di-, trienoic species. rest the phosphatide mass was contributed by 18, 32, 8% penta- hexaenoic respectively. proportions chemical classes...

Nine samples of fresh raw cow's milk were separated into fat globules and serum by centrifugation. After destabilization freezing thawing, the resolved membranes cores. The lipid composition these structures was compared to that surrounding serum. Of total fat, 95-98% in cores, 0.5-1% globule rest (1.5-4%) cores contained 88-93% triglyceride, 5.2-9.8% diglyceride, 1.5-7.3% free fatty acid 0.2-0.4% cholesterol, but no phospholipid. lipids membrane 21-44% phospholipid, made up about equal...

In the acute-phase response and in diseases with prolonged acute phases, normal HDL (NHDL) is converted into (APHDL) becomes proinflammatory unable to protect LDL against oxidative modification. Earlier work had demonstrated that these changes are associated alterations apolipoprotein composition enzymatic activity of APHDL, but effect condition on lipid APHDL remained obscure. The present study shows marked quantitative differences between NHDL APHDL. Specifically, contained 25% less total...

A membrane-associated sn-1,2(2,3)-diacylglycerol transacylase activity was purified 550-fold from the microsomes of rat intestinal villus cells. The enzyme solubilized with zwitterionic detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate and isolated by sequential chromatography on hydroxylapatite, an anion exchanger, Affi-Gel heparin Cibacron blue A-agarose. SDS-polyacrylamide gel electrophoresis revealed two major closely migrating polypeptides apparent molecular mass 50 52...