A5074886892- IL-33, ST2, and ILC Pathways
- Immune Response and Inflammation
- Alkaline Phosphatase Research Studies
- Drug Transport and Resistance Mechanisms
- Inflammatory Bowel Disease
- Pancreatitis Pathology and Treatment
- Liver Disease Diagnosis and Treatment
- Whipple's Disease and Interleukins
- Protein Hydrolysis and Bioactive Peptides
- Diet, Metabolism, and Disease
- Inflammasome and immune disorders
- Microscopic Colitis
- Helicobacter pylori-related gastroenterology studies
- Advanced Glycation End Products research
- Gut microbiota and health
- Biomarkers in Disease Mechanisms
- Immune cells in cancer
- Fatty Acid Research and Health
- Digestive system and related health
- Infant Nutrition and Health
- Seaweed-derived Bioactive Compounds
- Ion Transport and Channel Regulation
- Barrier Structure and Function Studies
- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
Universitätsklinikum Erlangen
2022-2025
Friedrich-Alexander-Universität Erlangen-Nürnberg
2020-2025
Universidad de Granada
2016-2025
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2016-2025
Instituto de Investigación Biosanitaria de Granada
2016-2025
German Climate Computing Centre
2022
Dysregulation at the intestinal epithelial barrier is a driver of inflammatory bowel disease (IBD). However, molecular mechanisms failure are not well understood. Here, we demonstrate dysregulated mitochondrial fusion in cells (IECs) patients with IBD and show that impaired sufficient to drive chronic inflammation. We found reduced expression fusion–related genes, such as dynamin-related guanosine triphosphatase (GTPase) optic atrophy 1 ( OPA1 ), fragmented networks crypt IECs IBD. Mice Opa1...
Objective We sought to investigate the role of interleukin (IL)-20 in IBD and experimental colitis. Design Experimental colitis was induced mice deficient components IL-20 signal transducer activator transcription (STAT)2 signalling pathways. In vivo imaging, high-resolution mini-endoscopy histology were used assess intestinal inflammation. further RNA-sequencing (RNA-Seq), RNAScope Gene Ontology analysis, western blot analysis co-immunoprecipitation, confocal microscopy epithelial cell...
Mutations in presenilin genes are the major cause of Alzheimer's disease. However, little is known about their expression and function gut. In this study, we identify presenilins Psen1 Psen2 as key molecules that maintain intestinal homoeostasis.
We investigated the effect of a high fructose diet (HFD) on Sprague Dawley rats and impact synbiotic composed Lactobacillus fermentum CECT5716 fructooligosaccharides. Feeding HFD for 5 weeks resulted in liver steatosis insulin resistance but not obesity. These changes were associated with increased production short‐chain fatty acids Bacteroidetes feces, an augmented Bacteroidetes/Firmicutes ratio, among other microbiota. In addition, barrier function was weakened, LPS plasma levels. data are...
Hydrolysates of food protein sources have immunomodulatory effects, which are interest for use as functional foods. In this study, we characterized the immune regulatory effect on rat splenocytes, macrophages and T lymphocytes Ulva spp. hydrolysates their peptide fractions with or without in vitro gastrointestinal digestion and/or ultrafiltration. IL-10 was induced almost all conditions cell types obtained from wild type animals. The induction general increased by ultrafiltration digestion....
Background IBD is a chronic inflammatory condition driven by complex genetic and immune interactions, yet preclinical models often fail to fully recapitulate all aspects of the human disease. A systematic comparison commonly used essential identify conserved molecular mechanisms improve translational relevance. Objective We performed multimodel transcriptomic analysis 13 widely mouse uncover coregulatory gene networks between colitis/ileitis define model-specific cellular, subcellular...
Regulation of miR-146a abundance and its role in intestinal inflammation particularly epithelial cells (IECs) has been poorly studied. Here we study the relationship between bacterial antigens inflammatory stimuli, expression using IEC lines models colitis (trinitrobenzenesulfonic acid (TNBS), dextran sulfate sodium (DSS) CD4 + CD62L T cell transfer model). Specific cytokines (LPS, flagelin IL-1β/TNF) stimulate expression, while peptidoglycan, muramyldipeptide CpG DNA have no effect....
Calprotectin (CP), a heterodimer of S100A8 and S100A9, is expressed by neutrophils number innate immune cells used widely as marker inflammation, particularly intestinal inflammation. CP ligand for toll-like receptor 4 (TLR4) the advanced glycation end products (RAGE). In addition, can act microbial modulatory agent via mechanism termed nutritional immunity, depending on metal binding, most notably Zn
Background: Enteric glial cells (EGC) play a crucial role in maintaining gut homeostasis, but their dysregulation inflammatory bowel diseases (IBD) remains poorly understood. Emerging preclinical data suggests activated EGC have beneficial roles controlling pathophysiology. Objective: Understanding activation and adaptation during experimental clinical IBD. Design: We provide the first highly integrated approach to identify signature Profiling 390 samples from IBD patients via bulk...
The development of inflammatory bowel diseases (IBD) involves the breakdown two barriers: epithelial barrier and gut-vascular (GVB). destabilization each can promote initiation progression disease. Interestingly, first evidence is available that both barriers are communicating through secreted factors may accordingly serve as targets for therapeutic modulation functions. Interferon (IFN)-γ among major pathogenesis in IBD severely impair barriers. In order to identify transmitting signals...
Two alkaline phosphatase isoforms, intestinal [IAP] and tissue non-specific [TNAP], are coexpressed in mouse colon, with the latter predominating colitis. We aimed to examine role of TNAP T lymphocytes, using heterozygous TNAP+/- mice [as TNAP-/- non-viable].In vitro primary cultures vivo cell models were used.Stimulated splenocytes [lipopolysaccharide concanavalin A] lymphocytes [concanavalin A a-CD3/a-CD28] showed a decreased cytokine production expression when compared wild-type [WT]...
Objective Psen1 was previously characterised as a crucial factor in the pathogenesis of neurodegeneration patients with Alzheimer’s disease. Little, if any, is known about its function gut. Here, we uncovered an unexpected functional role gut epithelial cells during intestinal tumourigenesis. Design Human colorectal cancer (CRC) and control samples were investigated for PSEN1 proteins theγ-secretase complex. Tumour formation analysed AOM-DSS Apc min/+ mouse models using newly generated...
Brewers' spent grain (BSG) is a relevant, protein-rich by-product of the brewing process. Protein hydrolysates from different sources exert immune-regulatory actions activating toll-like receptors (TLRs), nuclear factor kappa B (NFκB), and mitogen-activated protein kinases (MAPKs). Effects gastrointestinal digestion have been poorly studied. Here, we studied effect BSG hydrolysates, their in-vitro-digested products, on rat splenocytes, macrophages, T lymphocytes RESULTS: In primary cultures...
Introduction: Prematurity, a well-established risk factor for various intestinal diseases in newborns, results increased morbidity and mortality. However, the inflammatory status of preterm (PT) infants has been poorly characterized. Here we have broadly described systemic PT children. Materials Methods: Meconium plasma from 39 32 full term (T) newborns were studied. Fecal calprotectin, polymorphonuclear leukocyte elastase (PMN-E), TNF, IL-17A, IL-8, IP-10, MCP-1, MIP-1, IL-1β, IL-1α,...
The liver expresses tissue-nonspecific alkaline phosphatase (TNAP), which may participate in the defense against bacterial components, cell regulation as part of purinome or bile secretion, among other roles. We aimed to study role TNAP development hepatosteatosis. TNAP+/− haplodeficient and wild type (WT) mice were fed a control diet (containing 10% fat w/w) same deficient methionine choline (MCD diet). MCD induced substantial weight loss together with hepatic steatosis increased alanine...
Abstract Background Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the intestine with complex and multifaceted pathogenesis. While various animal models exist to study specific mechanisms relevant human IBD, comprehensive comparative framework linking these IBD pathophysiology lacking. Objective In our study, we aimed at providing that delineates common unique features encountered in 13 widely used mouse comparing them identify translatable pathways model-cohort...
The role of leptin in the development intestinal inflammation remains controversial, since proinflammatory and anti-inflammatory effects have been described. This study describes effect absence signaling inflammation. Experimental colitis was induced by intrarectal administration trinitrobenzene sulfonic acid (TNBS) to lean obese Zucker rats (n = 10). Effects on mucosal barrier were studied. Bacterial translocation LPS concentration evaluated together with colonic permeability 4-kDa...