A5045410651- Plant Virus Research Studies
- Nanopore and Nanochannel Transport Studies
- Bacteriophages and microbial interactions
- PARP inhibition in cancer therapy
- Calcium signaling and nucleotide metabolism
- RNA and protein synthesis mechanisms
- Advanced Fluorescence Microscopy Techniques
- Monoclonal and Polyclonal Antibodies Research
- Graphene research and applications
- Advanced biosensing and bioanalysis techniques
- Toxin Mechanisms and Immunotoxins
- Viral gastroenteritis research and epidemiology
- Mosquito-borne diseases and control
- Electrostatics and Colloid Interactions
- DNA and Nucleic Acid Chemistry
- Microfluidic and Bio-sensing Technologies
- Advanced Electron Microscopy Techniques and Applications
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Force Microscopy Techniques and Applications
- HIV Research and Treatment
University of Illinois Urbana-Champaign
2022-2024
Max Planck Institute of Biophysics
2024
In solution, DNA, the “most important molecule of life,” is a highly charged macromolecule that bears unit negative charge on each phosphate its sugar-phosphate backbone. Although partially compensated by counterions (cations solution) adsorbed at or condensed near it, DNA still produces substantial electric field in vicinity, which screened buffer electrolytes longer distances from DNA. This experienced any dipolar species approaching and interacting with So far, such has been explored...
ABSTRACT Accurate localization of biomolecules is pivotal for understanding biological processes. Utilizing the atomically flat surface 2D materials offers a promising route to achieve this without need tethering or constraining. Here we comprehensively investigate binding and diffusion DNA on hexagonal boron nitride (hBN) surfaces. Our findings reveal non-specific pristine hBN, with subsequent confinement within plane. Through single-molecule experiments computational techniques, explore...
Abstract The intricate interplay between DNA and proteins is key for biological functions such as replication, transcription, repair. To better understand these interactions, it crucial to develop tools study DNA-protein complexes with high spatial temporal resolution. Here, we use the vertical orientation that adopts on graphene investigate its interactions via energy transfer from a probe dye graphene, achieving resolution down Ångström scale. We measured bending angle of induced by...
<title>Abstract</title> Accurate localization and delivery of biomolecules is pivotal for building tools to understand biology. The interactions with atomically flat 2D surfaces offer a means realize both the delivery, yet experimental utilization such has remained elusive. By combining single-molecule detection methods computational approaches, we have comprehensively characterized individual DNA molecules hexagonal boron nitride (hBN) surfaces. Our experiments directly show that, upon...
Poly(ADP-ribose) (PAR), as part of a post-translational modification, serves flexible scaffold for noncovalent protein binding. Such binding is influenced by PAR chain length through mechanism yet to be elucidated. Structural insights have been elusive, partly due the difficulties associated with synthesizing chains defined lengths. Here, we employ an integrated approach combining molecular dynamics (MD) simulations small-angle X-ray scattering (SAXS) experiments, enabling us identify highly...