A5068213549- Fatty Acid Research and Health
- Immune Response and Inflammation
- Cell death mechanisms and regulation
- Inflammatory mediators and NSAID effects
- Lipid metabolism and biosynthesis
- Lipid Membrane Structure and Behavior
- Mitochondrial Function and Pathology
- Liver Disease Diagnosis and Treatment
- Osteoarthritis Treatment and Mechanisms
- Pharmacogenetics and Drug Metabolism
- Genomics, phytochemicals, and oxidative stress
- Protein Hydrolysis and Bioactive Peptides
- Viral gastroenteritis research and epidemiology
- Animal Genetics and Reproduction
- Veterinary Equine Medical Research
- Abdominal vascular conditions and treatments
- Moringa oleifera research and applications
- Animal Nutrition and Physiology
- Endoplasmic Reticulum Stress and Disease
- Diet, Metabolism, and Disease
- Infectious Disease Case Reports and Treatments
- Hepatitis B Virus Studies
- Immune cells in cancer
- TGF-β signaling in diseases
- Estrogen and related hormone effects
Leipzig University
2011-2023
Abstract Apoptosis acts in defense against microbial infection, and many infectious agents have developed strategies to inhibit host cell apoptosis. The human pathogen Chlamydia trachomatis ( Ctr ) is an obligate intracellular bacterium that strongly inhibits mitochondrial apoptosis of its but there no agreement how the bacteria achieve this. We here provide a molecular analysis chlamydial apoptosis-inhibition infected cells demonstrate block occurs during activation effectors apoptosis, Bak...
Although it is well recognized that dietary supplementation with fish oil improves clinical symptoms in dogs suffering from osteoarthritis, the molecular basis for benefit not yet completely resolved dogs. This study was designed to further clarify how polyunsaturated fatty acids (PUFA) affect key factors of cartilage degeneration a canine cell culture system mimicking osteoarthritis. Canine chondrocytes were incubated either without or 10 μm eicosapentaenoic acid (EPA), docosahexaenoic...
Nutritional fatty acids are known to have an impact on membrane lipid composition of body cells, including cells the immune system, thus providing a link between dietary acid uptake, inflammation and immunity. In this study we reveal significance macrophage gene expression cytokine synthesis thereby highlighting signal transduction processes, activation as well defense mechanisms. Using RAW264.7 macrophages model identified polyunsaturated (PUFA) both n-3 n-6 family down-regulate of: (i)...
It is well known that PUFA impede the LPS-mediated activation of transcription factor NFkappaB. However, underlying mode action has not been clarified yet. To address this issue in a comprehensive approach, we used monocyte/macrophage cell line RAW264.7 to investigate consequences supplementation on TLR4 pathway with focus (i) gene expression itself as its downstream mediators, (ii) membrane microdomain localization and CD14, (iii) stimulation-induced interaction CD14. Our data indicate...
Background Toll like receptors (TLRs) are an important and evolutionary conserved class of pattern recognition associated with innate immunity. The Gram-positive cell wall constituents strongly depends on TLR2. In order to be functional, TLR2 predominantly forms a heterodimer TLR1 or TLR6 within specialized membrane microdomains, the lipid rafts. composition physicochemical properties rafts subject modification by exogenous fatty acids. Previous investigations our group provide evidence that...
Abstract OBJECTIVE To determine effects of transforming growth factor (TGF)-β and interleukin (IL)-1β on inflammatory markers in cultured canine chondrocytes to clarify the role these cytokines osteoarthritis dogs. SAMPLE Pooled isolated from stifle joints 4 adult PROCEDURES Chondrocytes were isolated, cultured, frozen at −80°C. cells incubated medium with or without TGF-β (1 10 ng/mL) subsequently stimulated IL-1β (10 ng/mL). Concentrations nitric oxide (NO) prostaglandin (PG) E measured...
In recent years, hepatitis C virus (HCV)-related viruses were identified in several species, including dogs, horses, bats, and rodents. addition, a novel of the genus Hepacivirus has been discovered bovine samples was termed hepacivirus (BovHepV). Prediction BovHepV internal ribosome entry site (IRES) structure revealed strong similarities to HCV IRES comprising domains II, IIIabcde, pseudoknot IIIf, IV with initiation codon AUG. Unlike HCV, only one microRNA-122 (miR-122) binding could be...
The regulation of cytochrome P450 3A (CYP3A) enzymes is established in humans, but molecular mechanisms its basal and xenobiotic-mediated cattle are still unknown. Here, ~10 kbp the bovine CYP3A28 gene promoter were cloned sequenced, putative transcription factor binding sites predicted. proximal (PP; -284/+71 bp) contained DNA elements conserved among species. Co-transfection nuclear receptors (NRs) pregnane X constitutive androstane receptor (bPXR bCAR) with various constructs into...
Green liver discoloration (GL) in fattening turkeys is suspected to be a multifactorial disease complex with compromised immune system as the key factor. This study aimed identify formal pathogenesis of GL and investigate possible nutritional influences. A total 360 Bronze turkey hens out 10 flocks from 5 farms were necropsied for detection during 2 consecutive trials on examination dates each (70th 75th 120th 127th day fattening, respectively). At date, hematological clinical chemistry...
Hepatosteatosis is a common metabolic disorder of dairy cows, especially during early lactation. Currently, there are few models bovine hepatic steatosis available, including primary hepatocytes, liver slices, and animal models. Studies that elucidate the influence single fatty acids on lipid classes, acid pattern, gene expression, phenotypic changes still limited. Hence, we investigated suitability fetal hepatocyte-derived cell line BFH12 as model for hepatosteatosis. To create steatotic...
Osteoarthritis the quality and span of life in horses. Previous studies focused on nasal cartilage as a possible source for autologous chondrocyte implantation (ACI) defects humans. “HOX gene-negative” chondrocytes adapted articular HOX patterns after into caprine joint produced matrix proteins. We compared gene profile equine septum, anterior posterior fetlock to identify potential ACI Cartilage was harvested from seven horses death derived were cultured monolayer fourth subcultivation. A3,...