A5023309243- Plant Stress Responses and Tolerance
- Aluminum toxicity and tolerance in plants and animals
- Plant Micronutrient Interactions and Effects
- Photosynthetic Processes and Mechanisms
- Heavy metals in environment
- Heavy Metals in Plants
- Marine and coastal ecosystems
- Magnetic and Electromagnetic Effects
- Dye analysis and toxicity
- Nanoparticle-Based Drug Delivery
- Silicon Effects in Agriculture
- Biocrusts and Microbial Ecology
- Cassava research and cyanide
- Clay minerals and soil interactions
- Epoxy Resin Curing Processes
- Material Properties and Applications
- Iron oxide chemistry and applications
- Electrochemical Analysis and Applications
- Phosphorus and nutrient management
- Mechanical Behavior of Composites
- Microbial Community Ecology and Physiology
- Aquatic Ecosystems and Phytoplankton Dynamics
- Nanoplatforms for cancer theranostics
- Algal biology and biofuel production
- Bauxite Residue and Utilization
HTWG Hochschule Konstanz - Technik, Wirtschaft und Gestaltung
2024
Federal Institute For Materials Research and Testing
2024
Institute of Plant Molecular Biology
2016-2023
Czech Academy of Sciences, Biology Centre
2016-2023
Czech Academy of Sciences
2016-2023
University of Konstanz
2009-2016
University of South Bohemia in České Budějovice
2016
In-Q-Tel
2013
Metal accumulation in seeds is a prerequisite for germination and establishment of plants but also micronutrient delivery to humans. To investigate metal transport processes their interactions seeds, we focused on METAL TOLERANCE PROTEIN8 (MTP8), tonoplast transporter the manganese (Mn) subclade cation diffusion facilitators, which Arabidopsis (Arabidopsis thaliana) expressed embryos seeds. The x-ray fluorescence imaging showed that expression MTP8 was responsible Mn localization...
Chlorophyll fluorescence kinetic analysis has become an important tool in basic and applied research on plant physiology agronomy. While early systems recorded the integrated kinetics of a selected spot or plant, later enabled imaging at least slower parts (20-ms time resolution). For faster events, such as rise from dark-adapted yield to maximum (OJIP transient), decrease during reoxidation plastoquinone A after saturating flash, integrative are used because limiting speed available...
Arsenic (As) pollution is a serious concern worldwide. Recent studies under environmentally relevant conditions revealed that, in the aquatic plant Ceratophyllum demersum , pigments are first observable target of toxicity, prior to any effect on photosynthetic parameters or oxidative stress. Lethal toxicity was initiated by change As species and their distribution pattern various tissues. Here, localization investigated at subcellular level through X-ray fluorescence using submicron beam...
Summary Cadmium (Cd) is an important environmental pollutant and poisonous to most organisms. We aimed unravel the mechanisms of Cd toxicity in model water plant Ceratophyllum demersum exposed low (nM) concentrations as are present nature. Experiments were conducted under environmentally relevant conditions, including nature‐like light temperature cycles, a biomass ratio. measured chlorophyll (Chl) fluorescence kinetics, oxygen exchange, reactive species pigments, metal binding proteins,...
Solving the global environmental and agricultural problem of chronic low-level cadmium (Cd) exposure requires better mechanistic understanding. Here, soybean (Glycine max) plants were exposed to Cd concentrations ranging from 0.5 nM (background concentration, control) 3 µM. Plants cultivated hydroponically under non-nodulating conditions for 10 weeks. Toxicity symptoms, net photosynthetic oxygen production photosynthesis biophysics (chlorophyll fluorescence: Kautsky OJIP) measured in young...
The heavy metal cadmium (Cd) is highly toxic to plants. To understand the mechanisms of tolerance and resistance Cd, we treated rootless, submerged macrophyte Ceratophyllum demersum L. with sub-micromolar concentrations Cd under environmentally relevant conditions. X-ray fluorescence measurements revealed changing distribution patterns Zn at non-toxic (0.2 nM, 2 nM), moderately (20 nM) (200 levels Cd. Increasing led enhanced sequestration into non-photosynthetic tissues like epidermis vein....
Techniques for metal speciation analysis with subnanomolar (ppt) detection limits in complex matrices, simultaneous quantification of matrix elements, have become a necessity investigating targets trace binding to macromolecules and pigments at environmentally relevant concentrations. In this work we optimized the such custom-built HPLC-ICP-MS system. Key elements optimization were choice components metal-free HPLC-DAD system sector-field ICP-MS (ICF-sfMS) desolvating injection sample...
As the nonheterocystous diazotrophic cyanobacterium Trichodesmium lives both at ocean surface and deep in water column, it has to acclimate vastly different irradiances. Here, we investigate its strategy of light acclimation several ways. In this study, used spectrally resolved fluorescence kinetic microscopy biophysics photosynthesis individual cells, analysed cell extracts for pigment phycobiliprotein composition, measured nitrogenase activity abundance key proteins, assayed protein...
METAL TOLERANCE PROTEIN8 (MTP8) of Arabidopsis thaliana is a member the CATION DIFFUSION FACILITATOR (CDF) family proteins that transports primarily manganese (Mn), but also iron (Fe). MTP8 mediates Mn allocation to specific cell types in developing embryo, and Fe re-allocation as well tolerance during imbibition. We analysed if an overexpression driven by CaMV 35S promoter has effect on imbibition storage seeds, which would render it biofortification target. Fe, Zn concentrations...
Abstract Carbon fiber-epoxy laminates are used in aerospace manufacturing, e.g. as substrates for solar cells of satellites. Commonly, fibers or fibermats impregnated with epoxy resin and placed the required orientation. During subsequent curing, molecules crosslinked. Cured parts characterized by their glass transition temperature (T g ). It has been observed that T matrix vary recorded absolute air humidity during wet fiber placement manufacturing. Based on production data a series 203...