Xanthomonas oryzae pv. (Xoo) is a bacterial pathogen responsible for leaf blight (BLB) in rice, which can result significant yield losses of up to 70%. A study evaluated the spread Xoo rice fields using environmental samples and employed colorimetric loop-mediated amplification (cLAMP) PCR detection. Quantitative (qPCR) was used measure infection levels. The research compared severity between susceptible variety, Phitsanulok 2 (PSL2), resistant PSL2-Xa21. Results showed that decreased from...

Rice (Oryza sativa L.) is a vital global crop with predominant presence in Asia, including Thailand. However, it faces significant threat from bacterial blight disease (BB), primarily caused by Xanthomonas oryzae pv. (Xoo). This research aims to provide an insights into the genetic virulence and variation of Xoo strains isolated Our phylogenetic analysis unveils that 20 Thai align Asian strains, setting them apart African USA strains. Remarkably, Average Nucleotide Identity (ANI) values,...

Abstract. Sujipuli K, Mingmanit Y, Inthima P, Laywisadkul S, Ratanasut Premjet D, Pongcharoen Pathaichindachote W, Phinyo M, Boonsrangsom T. 2025. Biodiversitas 26: 125-135. Banana (Musa spp.) is a globally important fruit crop, with most edible varieties resulting from hybridizations between Musa acuminata (AA genome) and balbisiana (BB genome). Seed formation in hybrid bananas undesirable for both fresh consumption processing, making it crucial to understand the genetic mechanisms...

Abstract Salicylic acid (SA) serves as a pivotal signaling agent, activating defense mechanisms such hypersensitive responses and systemic acquired resistance in various plants. This study aims to investigate the impact of SA application on bacterial blight (BB) across diverse rice cultivars. The optimization spraying involved testing three distinct methods: Method Ⅰ (daily with for 2 consecutive days before inoculation), II (spraying once inoculation again 1 day after III inoculation). Each...

Dietary supplementation of plant herbs into aquafeed is recommended for intensive and sustainable aquaculture practices. This study has investigated the effect Persicaria odorata (PO) leaf powder on growth, feed utilization, whole‐body composition, hematology, immune‐related gene expression in Nile tilapia ( Oreochromis niloticus ). Fish (∼7.77 ± 0.01 g/fish) were randomly allocated four treatment groups, each with three replicates. They fed dietary PO at concentrations 0, 15, 30, 50 g/kg...

Rice (Oryza sativa L.) is a vital global crop with predominant presence in Asia, including Thailand. However, it faces significant threat from bacterial blight disease (BB), primarily caused by Xanthomonas oryzae pv. (Xoo). This research aims to provide an insights into the genetic virulence and variation of Xoo strains isolated Our phylogenetic analysis unveils that 20 Thai align Asian strains, setting them apart African USA strains. Remarkably, Average Nucleotide Identity (ANI) values,...

The broad-spectrum mildew resistance genes RPW8.1 and RPW8.2 define a unique type of plant disease (R) gene, so far homologous sequences have been found in Arabidopsis thaliana only, which suggests recent origin. In addition to RPW8.2, the RPW8 locus contains three homologs RPW8, HR1, HR2, HR3, do not contribute powdery resistance. To investigate whether has originated recently, if processes involved, we isolated analyzed syntenic loci from lyrata, Brassica rapa B. oleracea. A. lyrata four...

The floral-dip transformation, the simplest technique, is no requirement of tissue culture procedure, and can directly transfer interest gene into plant reproductive cells. It has been successfully applied to various species. In this study, optimal conditions a method for production transgenic rice variety RD41 were explored. simple effective inoculation medium was composed Murashige Skoog (MS) medium, 5% sucrose, 44 nmol/L benzylaminopurine, 0.075% surfactant Tween-20 with pH 5.7....

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Xanthomonas oryzae pv. (Xoo) is a widespread pathogen causing bacterial leaf blight (BLB) disease, devastating rice productivity in many cultivated areas of Thailand. A specific and simple method for Xoo detection required to improve surveillance disease transmission outbreak. This study developed recombinase polymerase amplification (RPA) assay assisted with CRISPR-cas12a (RAC) from cell suspension infected samples without DNA extraction. The efficiency the RAC system using either Xoo80 or...

Abstract Banana (Musa spp.) is a major global economic fruit crop. However, cross-pollination from other Musa cultivars grown in nearby plantations results seeded that exceeds market demand. This study investigated pollen viability and germination examined the expression profiles of development-related genes across seven genomes (AA, BB, AAA, BBB, AAB, ABB ABBB). Twenty-three were assessed for using lacto-aceto-orcein triphenyltetrazolium chloride staining methods. Results revealed obtained...

Oryza sativa L. ssp. indica (RD47 cultivar) is a major commercial rice variety known for its highly stable yields. However, it susceptible to bacterial blight disease caused by Xanthomonas oryzae pv. (Xoo). While previous research has focused on improving cultivars through breeding programs, no reports involved the interaction between Xoo infection and gene expression. This study aimed analyze relationship expression, focusing two resistance genes (Xa21 XIK1) one (OsSWEET14). Gene expression...

Xanthomonas oryzae pv. (Xoo) is a pathogenic bacterium responsible for bacterial blight (BB) disease in rice, primarily mediated by the interaction between plant and pathogen. The virulence mechanism involves activation of Sugars Will Eventually be Exported Transporter (SWEET) gene family rice transcription activator-like effectors derived from Xoo. BB resistance xa5 has been identified as one most effective genes against Thai Xoo isolates, but xa5-mediated resistance-breaking strains have...

Abstract. Boonsrangsom T, Fuenghoi C, Premjet D, Suvittawat K, Ratanasut Sujipuli K. 2023. Genetic relationships and genome verification of Thai banana cultivars using Random Amplification Polymorphic DNA (RAPD) markers. Biodiversitas 24: 3758-3765. Edible bananas plantains, belonging to the family Musaceae, genus Musa, represent one most important fruit crops, with an annual production more than 65 million tons worldwide. Bananas have several hybrid variations since they are descended from...

Natural herbicide is considered as a sustainable approach for weed management in agriculture. Here, allelopathic activities of Piper betle L. extract (BE) and known allelochemical eugenol (EU) were studied against rice associated weeds terms germination seedling growth. Five plant species including crop (Oryza sativa L.); dicot weed, false daisy (Eclipta prostrata (L.) three monocot weeds, barnyard grass (Echinochloa crus-galli P. Beauv.), swollen fingergrass (Chloris barbata Sw.), weedy f....

Activators of sesquiterpene synthase (STS) gene expression and production in Piper betle L. were examined using quantitative real time PCR gas chromatography mass spectrometry methods, the allelopathic activity untreated Fusarium solani-treated betel extracts was tested on seed germination shoot root growth Thai rice variety PSL2 (Oryza sativa cv. Phitsanulok 2) three dominant paddy weeds (Eclipta prostrata, Echinochloa crus-galli Chloris barbata). The results demonstrated that F. solani...

Abstract. Sunaryo W, Wahida, Idris SD, Pratama AN, Ratanasut K, Nurhasanah. 2020. Genetic relationships among cultivated and wild bananas from East Kalimantan, Indonesia based on ISSR markers. Biodiversitas 21: 824-832. Kalimantan is one of biodiversity centers for banana in including or bananas. This long-historical genetically contributed by the existence cultivars, local/indigenous varieties introduced accessions other regions Indonesia. The has played an important role socio-economic...