- Soybean genetics and cultivation
- Legume Nitrogen Fixing Symbiosis
- Plant pathogens and resistance mechanisms
- Agronomic Practices and Intercropping Systems
- Genetics and Plant Breeding
- Genetic Mapping and Diversity in Plants and Animals
- Plant Pathogens and Resistance
- Selenium in Biological Systems
- Lipid metabolism and biosynthesis
- Phytase and its Applications
- Smart Agriculture and AI
- Plant Genetic and Mutation Studies
- Remote Sensing in Agriculture
- Plant Disease Management Techniques
- Agricultural pest management studies
- Plant Micronutrient Interactions and Effects
- Insect-Plant Interactions and Control
- Plant Virus Research Studies
- Plant Disease Resistance and Genetics
- Plant Molecular Biology Research
- Spectroscopy and Chemometric Analyses
- Phytoestrogen effects and research
- Plant responses to water stress
- Rice Cultivation and Yield Improvement
- Trace Elements in Health
University of Nebraska–Lincoln
2015-2024
Iowa State University
1988-2021
Soybean [ Glycine max (L.) Merr.] yield, when regressed on water needed to replenish 0 100% seasonal evapotranspiration (ET), generates an estimate of season‐specific water‐use efficiency (WUE). The impact unpredictable deficits might be lessened if high‐yielding genotypes had a smaller beta. Our objective was determine the genetic basis beta and carbon isotope discrimination (CID), theorized indicator transpiration (TE). A ‘Minsoy’ × ‘Noir 1’ population 236 recombinant inbred lines (RILs),...
Collecting plant phenotypic data with sufficient resolution (in both space and time) accuracy represents a long standing challenge in science research, has been major limiting factor for the effective use of genomic crop improvement. This is particularly true breeding where collecting large-scale field-based phenotypes can be very labor intensive costly. In this paper we reported multi-sensor system high throughput phenotyping breeding. The comprised five sensor modules (ultrasonic distance...
Advances in genotyping technology, such as by sequencing (GBS), are making genomic prediction more attractive to reduce breeding cycle times and costs associated with phenotyping. Genomic selection has been studied several crop species, but no reports exist soybean. The objectives of this study were (i) evaluate prospects for using GBS a typical soybean program (ii) the effect marker imputation on accuracy. To achieve these objectives, set lines sampled from University Nebraska Soybean...
ABSTRACT Soybean improvement via plant breeding has been critical for the success of crop. The objective this study was to quantify genetic change in yield and other traits that occurred during past 80 yr North American soybean Maturity Groups (MGs) II, III, IV. Historic sets 60 MG 59 49 IV cultivars, released from 1923 2008, were evaluated field trials conducted 17 U.S. states one Canadian province 2010 2011. Averaged over 27 II 26 III site‐years data, estimated rates 23 kg ha –1 MGs 20...
Soybean [ Glycine max (L.) Merr.] seed protein is negatively correlated with oil and often yield. Our goal was to examine the basis for these correlations at a quantitative genetic locus (QTL) level. Seventy‐six F 5 –derived recombinant inbred lines (RILs) from mating of high‐protein (480 g per kg seed) G. accession PI 437088A high‐yield cultivar Asgrow A3733 (420 −1 content) were evaluated in six irrigation treatments (i.e., 100, 80, 60, 40, 20, 0% replacement weekly evapotranspiration...
Soybean [ Glycine max (L.) Merr.] seeds contain high levels of protein and oil useful for human consumption. Increasing emphasis in breeding programs to produce soybeans with specific or content specialty markets demands that more efficient manipulation these traits be achieved. The objective this study was evaluate eight different soybean populations from the midwestern USA genetic markers linked seed content. were derived at Univ. Minnesota, Nebraska, Purdue Univ.‐USDA‐ARS. Each population...
The sensitivity of soybean [ Glycine max (L.) Merr.] main stem node accrual to ambient temperature has been documented in greenhouse‐grown plants but not with field‐grown the north‐central United States. Biweekly V‐node and R‐stage, number, internode length, other traits were quantified an irrigated split‐plot, four‐replicate, randomized complete block experiment conducted Lincoln, NE, 2003–2004. Main plots early‐, mid‐, late‐May, mid‐June sowing dates. Subplots 14 cultivars maturity groups...
Phenotypic characterization of soybean event 335-13, which possesses oil with an increased oleic acid content (> 85%) and reduced palmitic (< 5%), was conducted across multiple environments during 2004 2005. Under these conditions, the stability novel fatty profile not influenced by environment. Importantly, 335-13 compromised in yield both irrigated non-irrigated production schemes. Moreover, seed characteristics, including total protein, as well amino profile, were altered a result large...
Abstract Soybean is the world’s leading source of vegetable protein and demand for its seed continues to grow. Breeders have successfully increased soybean yield, but genetic architecture yield key agronomic traits poorly understood. We developed a 40-mating nested association mapping (NAM) population 5,600 inbred lines that were characterized by single nucleotide polymorphism (SNP) markers six in field trials 22 environments. Analysis agronomic, SNP data revealed 23 significant marker-trait...
Field-based high throughput plant phenotyping has recently gained increased interest in the efforts to bridge genotyping and gap accelerate breeding for crop improvement. In this paper, we introduce a large-scale, integrated robotic cable-driven sensing system developed at University of Nebraska field research. It is constructed collect data from 0.4 ha field. The sensor payload 30 kg offers flexibility integrate user defined modules. Currently it integrates four-band multispectral camera,...
Summary Epigenetic variation has been associated with a wide range of adaptive phenotypes in plants, but there exist few direct means for exploiting this variation. RNA i suppression the plant‐specific gene, MutS HOMOLOG 1 ( MSH ), multiple plant species produces developmental changes accompanied by modulation defence, phytohormone and abiotic stress response pathways along methylome repatterning. This msh1‐ conditioned reprogramming is retained independent transgene segregation, giving rise...
Sclerotinia stem rot [caused by sclerotiorum (Lib.) de Bary] is considered the second most important cause of yield loss in soybean [ Glycine max (L.) Merr]. Soybean cultivars show variability susceptibility, but no complete resistance to disease has been reported and little information on genetics available. The objective this study was identify putative quantitative trait loci (QTLs) associated with soybean. Recombinant inbred lines (RILs) from five populations were developed crossing...
Genetic improvement toward optimized and stable agronomic performance of soybean genotypes is desirable for food security. Understanding how perform in different environmental conditions helps breeders develop sustainable cultivars adapted to target regions. Complex traits importance are known be controlled by a large number genomic regions with small effects whose magnitude direction modulated factors. Knowledge the constraints undesirable resulting from genotype interactions key objective...
Alkaline soils comprise 30% of the earth and have low plant-available iron (Fe) concentration, can cause deficiency chlorosis (IDC). IDC causes soybean yield losses $260 million annually. However, it is not known whether molecular responses to are equivalent supply. tolerant sensitive lines provide a contrast identify specific factors associated with IDC. We used RNA-seq compare gene expression under combinations normal pH (5.7) or alkaline (7.7, imposed by 2.5 mM bicarbonate, 8.2 5...
Iron deficiency chlorosis (IDC) is an abiotic stress in soybean that can cause significant biomass and yield reduction. IDC characterized by stunted growth yellowing interveinal of early trifoliate leaves. Scoring severity the field conventionally done visual assessment. The goal this study was to investigate usefulness RGB (Red Green Blue) images plots captured under condition for scoring. Sixty-four lines with four replicates were planted six fields over two years. Visual scoring (referred...
Sclerotinia stem rot of soybean [Glycine max (L.) Merr.], caused by the fungal pathogen sclerotiorum (Lib.) de Bary, recently has increased in importance northern U.S. production area. The objective our study was to determine effectiveness three different inoculation techniques predicting field reactions cultivars sclerotinia rot. Eighteen were tested six Michigan environments from 1994 1996 and greenhouse or laboratory with methods. inoculated placing infested oat (Avena sativa L.) seed...
Simple sequence repeat (SSR) markers with linkages to the Rps1 , Rps2 Rps3 Rps4 Rps5 and Rps6 loci that govern soybean [ Glycine max (L.) Merr.] resistance Phytophthora root rot (caused by megasperma Drechs. f. sp. glycinea Kuan Ervin) are desired. Near‐isogenic lines (NILs) of Clark or Williams, homozygous resistant ( RpsRps ) at just one those Rps loci, were mated a NIL Harosoy susceptible rpsrps all six loci. From 100 120 F 2:3 progenies per mating, 20 3 seedlings evaluated for (R)...
During the past 50 yr, we have witnessed a revolution in science of plant breeding, genetics, and cytology, its impact on human lives (e.g., Green Revolution). Because increased productivity, breeding objectives evolved from predominantly improving yield to include greater quality value‐added traits. The discovery chemical nature deoxyribonucleic acid (DNA), coupled with Mendelian genetics led refinement quantitative robust use molecular markers, transgenic crop plants. Cytogenetics...
Abstract Plant breeders continually generate ever-higher yielding cultivars, but also want to improve seed constituent value, which is mainly protein and oil, in soybean [Glycine max (L.) Merr.]. Identification of genetic loci governing those two traits would facilitate that effort. Though genome-wide association offers one such approach, selective genotyping multiple biparental populations a complementary alternative, was evaluated here, using 48 F2:3 (n = ∼224 plants) created by mating...
Abstract Soybean ( Glycine max ) is the most widely grown oilseed in world and an important source of protein for both humans livestock. adapted to temperate tropical regions, but a changing climate demands better understanding adaptation specific environmental conditions. Here, we explore genetic variation collection 3,012 georeferenced, locally landraces from broad geographical range help elucidate basis local adaptation. We used geographic origin, data dense genome-wide SNP perform...
Abstract Global crop production is facing the challenge of a high projected demand, while yields major crops are not increasing at sufficient speeds. Crop breeding an important way to boost productivity, however its improvement rate partially hindered by long generation cycles. If end-season traits such as yield can be predicted through early-season phenotypic measurements, selection potentially made before full cycle finishes. This study explored possibility predicting soybean color and...