Spodoptera frugiperda (J.E. Smith) is composed of two genetically distinct strains, the so-called corn strain and rice strain. Whether strains differ in their host use unclear, because laboratory experiments have not been able to show consistent performance or preference differences between them, field studies showed high rates hybridization, as well some degree asymmetric use. To determine distribution association with plants, we collected fall armyworm larvae from different crops (corn,...

The method to assess the sexual compatibility and mating performance of Mediterranean fruit fly, Ceratitis capitata (Wiedemann), mass-reared strains has been revised. Three new indices (isolation index, ISI; male relative MRPI; female FRPI) that look at impact both population on isolation between laboratory wild are described. methodology were used in Argentina test SEIB 6–96 genetic sexing strain competition with C. from Patagonia region. experiments show 2 populations sexually compatible...

The genus Prosopis is an important member of arid and semiarid environments around the world.To study diversification evolution, a combined approach including molecular phylogeny, dating, character optimization analysis was applied.Phylogenetic relationships were inferred from five different markers G3pdh, NIA).Taxon sampling involved total 30 species that represented all Sections Series complete geographical range genus.The results suggest not natural group.Molecular dating indicates...

Spodoptera frugiperda, the fall armyworm, is a major economic pest throughout Western Hemisphere of corn (maize), cotton, sorghum, and variety agricultural grasses vegetable crops. Studies in United States, Caribbean, Brazil demonstrated existence two subpopulations (previously designated "host strains") that differ their choice plant host. Specifically, strain preferentially found while rice dominant rice, turf grass, alfalfa. However, inconsistent results were reported surveys armyworm...

The effects of routine irradiation the mass-reared males medfly, Ceratitis capitata on their mating performance were re-evaluated. Male courtship behavior was observed and quantified both in laboratory (video recording cages) field cage conditions. For experiments, samples strains routinely for SIT operations at Seibersdorf, Austria; Mendoza, Argentina Metapa, Mexico, used. No major qualitative differences found pattern between irradiated non-irradiated males. However, results revealed that...

Abstract Determining which factors contribute to the formation and maintenance of genetic divergence evaluate their relative importance as a cause biological differentiation is among major challenges in evolutionary biology. In S podoptera frugiperda ( mith) L epidoptera: N octuidae) two host strains have been recognized 1980s: corn‐strain prefers maize, sorghum, cotton, whereas rice‐strain rice wild grasses. However, it not clear what extent these so‐called ‘strains’, also called ‘host...

Anastrepha fraterculus (Wiedemann) (Diptera: Tephritidae) displays a lek mating system. Males form groups in which they simultaneously display signals (acoustical, visual, or chemical) to attract females with the purpose of mating. Females visit and choose among signaling courting males mate. Scarce information is available A. about main factors involved female choice behavior displaying males. This could be important within context pest control programs sterile insect technique (SIT)...

Ongoing discussion on the taxonomic status of Anastrepha fraterculus (Wiedemann) and limited biological knowledge this species emphasize a need for information sexual behavior mating compatibility among populations from different ecological regions. In study, we studied four A. fruit-growing biogeographical areas in Argentina, i.e., Yuto Horco Molle northwest Posadas Concordia northeast. Pair-wise tests were performed all possible combinations. Virgin males females, proportion 1:1, released...

As only six useful microsatellite loci that exhibit broad cross-amplification are so far available for Prosopis species, it is necessary to develop a larger number of codominant markers population genetic studies. Simple sequence repeat (SSR) obtained species from 454 pyrosequencing run were optimized and characterized studies in P. alba chilensis. •Twelve successfully amplified showed polymorphism The alleles per locus ranged between two seven heterozygosity estimates 0.2 0.8. Most these...

The remating behavior of wild Ceratitis capitata (Wiedemann) females was examined after first mating to males from the or one two genetic sexing strains: Cast 191 (irradiated nonirradiated males) Seib 6-96 only). observations were carried out in field cages set over a rooted host tree during continuous period 10 h which individually identified. On average, 12% remated. general trend higher rate for mated with lab compared males. It observed that remated tended start and end their earlier...

The effects of male size and other morphometric traits as determinants mating success were evaluated under field cage conditions. Males the laboratory Seib6-96 strain released into cages with males females a wild population from Patagonian region. Mating pairs classified 'successful', while unmated flies labeled 'unsuccessful'. Five measured in sample 141 unsuccessful 149 successful males: eye length (EL), head width (HW), thorax (TL), face (FW), wing (WL). An exploratory non-parametric...

We evaluated under semi-natural field cage conditions sexual compatibility and competitiveness of a laboratory strain (LAB) compared to wild population (TUC) Anastrepha fraterculus (Wiedemann). The LAB is produced semi-mass rearing at the Estación Experimental Agroindustrial Obispo Colombres facility (Tucumán, Argentina). Wild flies were obtained Horco Molle Argentina) from infested guava fruits. pupae irradiated (60Co) 48 h before adult emergence. tested doses 0 (control), 40, 70, 100 Gy....

The integration of ecology and genetics has become established in recent decades, hand with the development new technologies, whose implementation is allowing an improvement tools used for data analysis. In a landscape context, integrative management population information from different sources can make spatial studies involving phenotypic, genotypic environmental simpler, more accessible faster. Tools exploratory analysis autocorrelation help to uncover genetic structure populations...

Abstract Aim The genus Prosopis includes 44 species and has a pseudoamphitropical, disjunct distribution. We aimed to determine whether American sections arose in North or South America, explain the current distribution of their on basis genetic relationships. Location South‐western USA, Mexico, Caribbean Antilles, Peru–Ecuador, central northern Argentina, south‐western Argentina (Patagonia) Cuyo, Asia Africa. Methods Internal transcribed spacer fragments from 21 were sequenced data used...

Small inversions (SIs) in the chloroplast genome of angiosperms are ubiquitous. These always flanked by inverted repeats (palindromes or quasipalindromes) between approximately 8 and 50 bp long that form a hairpin structure when DNA is single-stranded. We evaluated different methodological empirical issues about SI evolution. As case study, we analysed an recently discovered psbC-trnS intergenic region Prosopis (Fabaceae). First, how can be optimized cases where segment also shows indels...

In relation to the application of sterile insect technique (SIT) for South American fruit fly Anastrepha fraterculus (Wiedemann), we analyzed effect on adult fertility different doses gamma irradiation and age pupae at time irradiation. a first experiment, applied 50, 70, 90 Gy 24, 48, 72, 96 h before emergence. second experiment irradiated 48 emergence with 20, 40, 60 estimated male female sperm transfer by males. The results indicated pupal does not significantly affect fertility. If males...

Anastrepha fraterculus (Wiedmann), a South American fruit fly, is an economically important pest for fruit-bearing plants of the neotropical region. Some evidence indicates that nominal A. comprises multiple cryptic species. In current work we analyzed genetic variability and structure in Argentine populations based on eight isoenzymatic loci restriction patterns fragment ≈500 bp belonging to 16 S mitochondrial ribosomal DNA. The analysis indicated significant excess homozygotes (FIS > 0),...

Morphological and genetic diversity among Acacia aroma , A. macracantha caven furcatispina were studied with morphometric, isozymal, RAPD approaches. The analysis of seven isozyme systems revealed 21 loci, showed 34 loci. Most these loci allowed us to differentiate the species, exception two most similar species. levels variability estimated by isozymes higher than those obtained from analyses. Morphometric characters highly significant differences although are differentiated only thorn...

The objective of the study was to compare courtship behavior various wild and mass reared medfly strains, in order document degree diversity among populations assess its implications for strategy application Sterile Insect Technique. Recordings were collected from several locations world-wide using a standard protocol. Qualitative quantitative analysis behavioral materials conducted. No major differences found strains both male female repertoire, which indicates general lack incompatibility...

Prosopis represents a valuable forest resource in arid and semiarid regions. Management of promising species requires information about genetic parameters, mainly the heritability (h(2)) quantitative profitable traits. This parameter is traditionally estimated from progeny tests or half-sib analysis conducted experimental stands. Such an approach estimates h(2) ratio between-family/total phenotypic variance. These analyses are difficult to apply natural populations with long life cycle,...