A5057187455- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- RNA modifications and cancer
- Cancer-related gene regulation
- Kruppel-like factors research
- Virus-based gene therapy research
- Cell Adhesion Molecules Research
- Blood disorders and treatments
- Pineapple and bromelain studies
- Plant tissue culture and regeneration
- RNA Interference and Gene Delivery
- Herpesvirus Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Bioeconomy and Sustainability Development
- Cancer Genomics and Diagnostics
- NF-κB Signaling Pathways
- Metabolism, Diabetes, and Cancer
- Fibroblast Growth Factor Research
- Lung Cancer Treatments and Mutations
- TGF-β signaling in diseases
University of Toronto
2015-2023
Hebrew University of Jerusalem
2009-2018
Canada Research Chairs
2015
Délégation Paris 5
2012
University Hospital Olomouc
2012
Palacký University Olomouc
2012
Descartes (Belgium)
2012
Université Paris Cité
2012
In light of the numerous studies identifying post-transcriptional regulators on surface endoplasmic reticulum (ER), we asked whether there are factors that regulate compartment specific mRNA translation in human cells. Using a proteomic survey spatially regulated polysome interacting proteins, identified glycolytic enzyme Pyruvate Kinase M (PKM) as cytosolic (i.e. ER-excluded) interactor and investigated how it influences translation. We discovered PKM-polysome interaction is directly by ADP...
Vav1 functions as a signal transducer protein in the hematopoietic system, where it is exclusively expressed. was recently implicated several human cancers, including lung, pancreatic and neuroblasoma. In this study, we analyzed expression function of breast tumors cancer cell lines. Immunohistochemical analysis primary carcinomas indicated that expressed 62% 65 tested correlated positively with estrogen receptor expression. Based on published gene profiling 50 lines, Vav1-expressing lines...
Vav1 is a signal transducer protein that functions as guanine nucleotide exchange factor for the Rho/Rac GTPases in hematopoietic system where it exclusively expressed. Recently, was shown to be involved several human malignancies including neuroblastoma, lung cancer, and pancreatic ductal adenocarcinoma (PDA). Although some factors affect vav1 expression are known, neither physiological nor pathological regulation of completely understood. We demonstrate herein mutations putative...
While one pathway for the post-translational targeting of tail-anchored (TA) proteins to endoplasmic reticulum (ER) has been well defined, it is unclear whether additional pathways exist. Here we provide evidence that a subset mRNAs encoding TA-proteins, such as Sec61β and Nesprin2, partially localized surface ER in mammalian cells. In particular, mRNA can be targeted to, later maintained on using both translation-dependent independent mechanisms. Our data suggests this process p180, known...
// Shulamit Sebban 1,* , Marganit Farago Shiran Rabinovich 1 Galit Lazer Yulia Idelchuck Lena Ilan Eli Pikarsky 2 and Katzav Departement of Developmental Biology Cancer Research, Institute for Medical Research Israel-Canada, Hadassah School - Hebrew University, Jerusalem, Israel Department Immunology & Pathology, * These authors contributed equally to this work Correspondence: Katzav, email: Keywords : Vav1, CSF1, Lung Cancer, Growth Factors Received June 16, 2014 Accepted August 26,...
A critical step in the cellular stress response is transient activation of RNA-dependent protein kinase PKR by double-helical RNA, resulting down-regulation synthesis through phosphorylation α chain translation initiation factor eIF2, a major substrate. However, intragenic elements 100-200 nucleotides length within primary transcripts genes, exemplified tumor necrosis (TNF)-α gene and fetal adult globin are capable forming RNA structures that potently activate thereby strongly enhance mRNA...
Abstract How human cells coordinate various metabolic processes, such as glycolysis and protein translation, remains unclear. One key insight is that enzymes have been found to associate with mRNAs, however whether these regulate mRNA biology in response changes cellular state unknown. Here we report the glycolytic enzyme, pyruvate kinase M (PKM), inhibits translation of 7% transcriptome elevated levels glucose pyruvate. Our data suggest presence pyruvate, PKM associates ribosomes are...