A5085941909- Cancer Cells and Metastasis
- RNA Interference and Gene Delivery
- Ovarian cancer diagnosis and treatment
- Immunotherapy and Immune Responses
- Cancer Research and Treatments
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Ferroptosis and cancer prognosis
- Cervical Cancer and HPV Research
- Microbial Community Ecology and Physiology
- Enzyme Production and Characterization
- Bacteriophages and microbial interactions
- Circular RNAs in diseases
- MicroRNA in disease regulation
- CAR-T cell therapy research
- Cancer, Hypoxia, and Metabolism
- Digestive system and related health
- RNA modifications and cancer
- Bacterial biofilms and quorum sensing
- Reproductive System and Pregnancy
- Gut microbiota and health
- Epigenetics and DNA Methylation
- Probiotics and Fermented Foods
University of Ulsan
2018-2025
Asan Medical Center
2018-2025
Ulsan College
2018-2024
Pusan National University
2012
Objective To investigate the anti-cancer efficacy of ENB101-LNP, an ionizable lipid nanoparticles (LNPs) encapsulating siRNA against E6/E7 HPV 16, in combination therapy with cisplatin cervical cancer vitro and vivo . Methods CaSki cells were treated cisplatin, or combination. Cell viability assessed cytotoxicity treatment. HPV16 gene knockdown was verified RT-PCR both HLA class I PD-L1 checked by flow cytometry. A xenograft model made using BALB/c nude mice. evaluate anticancer efficacy,...
Abstract Background: Ovarian cancer (OC) is a highly treatment-resistant malignancy, often diagnosed late due to minimal symptoms and extensive metastasis. Despite platinum-based chemotherapy, recurrence chemoresistance highlight the need for new therapies. This study focuses on cancer-associated fibroblasts (CAFs), key TME components, explore their impact OC progression identify novel CAF subtypes. Methods: CAFs were isolated from patient tumors via enzymatic dissociation flow cytometry....
Abstract Background: Recently, immunotherapy with immune checkpoint inhibitors (ICIs) has shown promising efficacy in endometrial cancer (EC), particularly patients mismatch repair deficiency (dMMR). Preclinical models of EC are essential for evaluating the immuno-oncology (IO) drugs and investigating strategies to enhance antitumor responses immunotherapy. Humanized (Hu) mouse can contribute a better understanding underlying mechanisms associated improved ICIs treating EC. Methods: We...
Abstract The regulatory properties of pyruvate kinase M2 isoform (PKM2), the key glycolytic enzyme, influence altered energy metabolism including glycolysis in cancer. In this study, we found that PKM2 was highly expressed patients with ulcerative colitis or colorectal cancer (CRC). We then investigated effectiveness conditionally ablating Lgr5 + intestinal stem cells (ISC) using a mouse model colitis-associated CRC (AOM plus DSS). Tamoxifen-inducible Lgr5-driven deletion ISC (PKM2 ΔLgr5...
Immunoprofiling has an established impact on the prognosis of several cancers; however, its role and definition in high-grade serous ovarian cancer (HGSOC) are mostly unknown. This study is to investigate immunoprofiling which could be a prognostic factor HGSOC. We produced tumor microarrays 187 patients diagnosed with performed multiplexed immunofluorescence staining using Opal Multiplex IHC kit quantitative analysis Vectra-Inform system. The expression intensities programmed death-ligand 1...
Abstract Dendritic cell (DC)-based immunotherapies have been shown to be a potential treatment option for various cancers; however, the exact strategies in ovarian cancer remain unknown. Here, we report effectiveness of mouse CD8α+ DCs derived from bone marrow hematopoietic stem cells (BM-HSCs), equivalent human CD141+ DCs, which proven highly superior subset. Mono-DCs monocytes and stem-DCs HSCs were characterized by CD11c+ CD80+ CD86+ Clec9a+ expression, respectively. Despite lower dose...
Abstract Background: Although immunotherapy has not yet been as successful in ovarian cancer (OC), it remains a potential therapeutic strategy. Preclinical models of OC are necessary to evaluate the efficacy immuno-oncology (IO) drugs targeting human and immune components but have underutilized. Developing mouse with humanized (Hu) system can help understand response IO drugs, including checkpoint inhibitors (ICIs), which demonstrated limited effectiveness patients. Methods: We established...
Although immunotherapy has not yet been as successful in ovarian cancer (OC), it remains a potential therapeutic strategy. Preclinical models of OC are necessary to evaluate the efficacy immuno-oncology (IO) drugs targeting human immune components but have underutilized. Developing mouse with humanized (Hu) system can help understand response IO which demonstrated limited effectiveness patients. We established xenograft Hu-mouse by intraperitoneally injecting luciferase-expressing SKOV-3 Luc...
환경에서 분리된 CK214 균주는 1.5% (w/v) agar가 포함되어 있는 LB 평판배지에서 빠르게 이동하는 특징을 가지며, agar 고체평판배지 위의 균주의 집락 주위로 움푹한 투명환이 관찰되었다. 이 단일 탄소원으로 agar만이 첨가된 최소 배지에서 잘 자랐으며, DNS 법을 이용하여 외부추출성분이 분해활성을 가진다는 것을 확인하였다. 다양한 농도의 (0.5, 1.0, 1.5 2.0% w/v)가 포함된 고체평판 swarming 운동을 하였다. 균주를 동정하기 위해 그람염색과 현미경 관찰, 생화학적 분석(API), 16S rRNA 염기서열분석에 기초한 계통발생학적 분석을 수행하였다. 이를 통해 그람 양성의 간균으로, Paenibacillus 속에 포함되었으며 lactis MB 2035와 가장 가까운 연관성을 보이는 확인할 수 있었다. 또한 고체표면에서 주모성의 편모를 형성하는 투과 전자 현미경(TEM)을 관찰하였다. agarase 활성과 운동성의 연관성에 관한 앞으로의...
Paenibacillus는 호기성의 내생포자를 형성하는 그람양성균으로써 이전에는 Bacillus로 분류되었다. Paenibacillus sp. CK214 균주는 LB agar 평판배지에서 높은 swarming 운동 능력을 가지고 특유의 집락 형태를 나타내었지만 glucose가 첨가된 평판배지에서는 상실하였다. 투과전자현미경(TEM)을 이용하여 glucose 조건에 따른 균주의 편모를 관찰하면 배양한 주모성의 가지는 반면 glucose를 첨가한 경우 주모성 편모가 나타나지 않는 것을 확인할 수 있었다. 물리적 충격과 원심분리를 통해 분리한 filament 구성 단백질을 SDS-PAGE를 확인하였으며, 약 29 kDa 크기의 단일 단백질 밴드가 나타났다. Edwardsiella tarda flagellin 단백질에 특이적인 항체를 이용한 immunoblotting 수행 결과, 이 밴드는 단백질임이 확인되었다. Glucose조건에 단백질의 발현을 수준에서 관찰한 조건에서 생장한...
본 연구는 아동의 놀이 환경이 삶의 만족도에 미치는 영향과 스트레스의 매개효과를 확인하기 위해 수행되었다. 이를 한국청소년정책연구원에서 수행한「청소년이 행복한 지역사회 지표조사 및 조성사업 연구」7차 자료를 활용하였으며, 분석 방법으로는 PROCESS macro for SPSS를 활용하여 매개효과 분석을 진행하였다. 통한 주요 연구결과는 다음과 같다. 첫째, 환경은 유의미한 정적 영향을 주는 것으로 나타났다. 둘째, 전반적 환경 만족도 또한 따라서 긍정적 요인이다. 셋째, 스트레스는 매개하는 연구를 통해 삶에서 환경의 중요성을 다시 확인하였으며, 보장을 위한 정책 실천적 제언을 제시하였다.
Abstract Background: High-risk human papillomavirus (HPV) type 16 is the major etiological agent found in more than 60% of patients with cervical cancer and associated development progression. Persistent expression HPV E6 E7 oncoproteins are essential for initiation maintenance cancer. The therapeutic approaches targeting have been proved to be highly efficient killing malignant cells. suppression E6/E7 by short interfering RNA (siRNA) was effective combination therapy cisplatin (CDDP)...
Abstract Objectives: Dendritic cell (DC) based immunotherapies have been shown to be a potential treatment option for various cancers; however, the exact strategies in ovarian cancer remain unknown. This study aimed evaluate effectiveness of mouse CD8α+ dendritic cells (DCs), corresponding human CD141+ DCs, derived from bone marrow (BM) hematopoietic stem (HSCs) syngeneic and orthotopic model. Methods: Stem-DCs HSCs Mono-DCs monocytes were generated mononuclear (BM-MNCs) C57BL/6 mice...
Objective: This study is to investigate the anti-cancer efficacy of ENB101-LNP, an ionizable lipid nanoparticles (LNPs) encapsulating siRNA against E6/E7 human papillomavirus (HPV) 16, in combination therapy with radiation cervical cancer xenograft model.Methods: The model was generated by injection CaSki cells subcutaneously into BALB/c nude mice.Mice were randomly divided 6 groups according ENB101-LNP.ENB101-LNP treated intravenous 3 times weekly for weeks and 5 Gy once at 14 days after...
ABSTRACT Background Although immunotherapy has not yet been as successful in ovarian cancer (OC), it remains a potential therapeutic strategy. Preclinical models of OC are necessary to evaluate the efficacy immuno-oncology (IO) drugs targeting human and immune components but have underutilized. Developing mouse with humanized (Hu) system can help understand response IO drugs, including checkpoint inhibitors (ICIs), which demonstrated limited effectiveness patients. Methods We established...