A5023526937- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- TGF-β signaling in diseases
- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- Renal function and acid-base balance
- Ubiquitin and proteasome pathways
- Enzyme function and inhibition
- Pharmaceutical studies and practices
- Potassium and Related Disorders
East Kent Hospitals University NHS Foundation Trust
2022
University of Oxford
2019
Abstract The role of Notch signaling and its ligand JAGGED1 (JAG1) in tumor biology has been firmly established, making them appealing therapeutic targets for cancer treatment. Here, we report the development characterization human/rat-specific JAG1-neutralizing mAbs. Epitope mapping identified their binding to receptor interaction site within JAG1 Delta/Serrate/Lag2 domain, where E228D substitution prevented effective murine Jag1 ortholog. These antibodies were able specifically inhibit...
<p>1: JAG1 antibody characterization. 2: inhibition of mediated Notch signalling in tumor cell lines. 3: vascular cells. 4: HUVEC-HUVSMC co-culture. 5: effect anti-Notch pathway treatment on MDA-MB231 2D growth and J1-65D titration 3D gene expression. 6: established OVCAR3 xenograft. 7: antibodies cross-reactivity with rat Jag1. 8: anti-JAG1 a brain metastasis model.</p>
<p>Supplementary material and methods. Supplementary table 1: cell lines growth conditions. 2: Notch ligands control protein for plate coating. 3: qPCR primers. 4: flow cytometry antibodies. 5: ICC/IHC 6: titration of Notch1-JAG1 binding inhibition by JAG1 7: amino acid sequence 8: blood analysis on antibody-treated tumor-bearing rats.</p>
<p>Supplementary material and methods. Supplementary table 1: cell lines growth conditions. 2: Notch ligands control protein for plate coating. 3: qPCR primers. 4: flow cytometry antibodies. 5: ICC/IHC 6: titration of Notch1-JAG1 binding inhibition by JAG1 7: amino acid sequence 8: blood analysis on antibody-treated tumor-bearing rats.</p>
<p>1: JAG1 antibody characterization. 2: inhibition of mediated Notch signalling in tumor cell lines. 3: vascular cells. 4: HUVEC-HUVSMC co-culture. 5: effect anti-Notch pathway treatment on MDA-MB231 2D growth and J1-65D titration 3D gene expression. 6: established OVCAR3 xenograft. 7: antibodies cross-reactivity with rat Jag1. 8: anti-JAG1 a brain metastasis model.</p>
<div>Abstract<p>The role of Notch signaling and its ligand JAGGED1 (JAG1) in tumor biology has been firmly established, making them appealing therapeutic targets for cancer treatment. Here, we report the development characterization human/rat-specific JAG1-neutralizing mAbs. Epitope mapping identified their binding to receptor interaction site within JAG1 Delta/Serrate/Lag2 domain, where E228D substitution prevented effective murine Jag1 ortholog. These antibodies were able...
<div>Abstract<p>The role of Notch signaling and its ligand JAGGED1 (JAG1) in tumor biology has been firmly established, making them appealing therapeutic targets for cancer treatment. Here, we report the development characterization human/rat-specific JAG1-neutralizing mAbs. Epitope mapping identified their binding to receptor interaction site within JAG1 Delta/Serrate/Lag2 domain, where E228D substitution prevented effective murine Jag1 ortholog. These antibodies were able...