A5013224445- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Animal Virus Infections Studies
- Molecular Biology Techniques and Applications
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- RNA modifications and cancer
- Enterobacteriaceae and Cronobacter Research
- Neuroscience and Neural Engineering
- Agriculture Sustainability and Environmental Impact
- Aquaculture disease management and microbiota
- Clinical Nutrition and Gastroenterology
- Membrane Separation Technologies
- MicroRNA in disease regulation
- Stock Market Forecasting Methods
- Forecasting Techniques and Applications
- Animal Genetics and Reproduction
- Innovative Microfluidic and Catalytic Techniques Innovation
National Institute for Bioprocessing Research and Training
2018-2024
Dublin City University
2010-2020
University of Limerick
2016-2019
To study the role of microRNA (miRNA) in regulation Chinese hamster ovary (CHO) cell growth, qPCR, microarray and quantitative LC-MS/MS analysis were utilised for simultaneous expression profiling miRNA, mRNA protein. The sample set under investigation consisted clones with variable cellular growth rates derived from same population. In addition to providing a systems level perspective on integration multiple datasets can facilitate identification non-seed miRNA targets, complement...
Improving the efficiency of recombinant protein production by CHO cells is highly desirable as more complex proteins (MAbs, fusion proteins, blood/clotting factors, etc.) go into development and come onto market. Previous reports have shown that microRNA (miRNA)-7 overexpression arrests growth its depletion increases proliferation various cell types. In this study we generated stable clones overexpressed a miR-7-specific decoy transcript (sponge) downstream green fluorescent reporter gene....
Abstract microRNA engineering of CHO cells has already proved successful in enhancing various industrially relevant phenotypes and producing recombinant products. A single miRNA's ability to interact with multiple mRNA targets allows their regulatory capacity extend processes such as cellular metabolism. Various metabolic states have previously been associated particular cell glycolytic or oxidative metabolism accommodating growth productivity, respectively. miR‐23 demonstrated play a role...
The biopharmaceutical industry has invested considerably in the implementation of single-use disposable bioreactors place or addition to their stainless steel-counterparts. This new wave construction materials for bioprocess containers encompass a plethora uncharacterized secondary compounds that, when contact with culture media, can leach, contaminating bioprocess. One such cytotoxic leachable already receiving attention is bis(2,4-di-tert-butylphenyl)-phosphate (bDtBPP), breakdown product...
Abstract Single‐use technologies, in particular disposable bioreactor bags, have become integral within the biopharmaceutical community. However, safety concerns arose upon identification of toxic leachable compounds derived from plastic materials. Although bis(2,4‐di‐tert‐butylphenyl)‐phosphate (bDtBPP) has been previously shown to inhibit CHO cell growth, it is critical determine if other like this are still present subsequent generations films for industrial application. This study...
Abstract RNA sequencing (RNASeq) has been widely used to associate alterations in Chinese hamster ovary (CHO) cell gene expression with bioprocess phenotypes; however, alternative messenger (mRNA) splicing, thus far, received little attention. In this study, we utilized RNASeq for transcriptomic analysis of a monoclonal antibody (mAb) producing CHO K1 line subjected temperature shift. More than 2,465 instances differential splicing were observed 24 hr after the reduction culture temperature....
Genetic engineering of mammalian cells is interest as a means to boost bio‐therapeutic protein yield. X‐linked inhibitor apoptosis (XIAP) overexpression has previously been shown enhance CHO cell growth and prolong culture longevity while additionally boosting productivity. The authors confirmed this across range recombinant products (SEAP, EPO, IgG). However, stable an transgene competes for the translational machinery potentially compromising product titre. MicroRNAs are attractive genetic...
Fundamental to the efficient production of quality biopharmaceuticals is selection, optimization and tailored manipulation mammalian cellular host. Engineering these cell factories, predominantly Chinese hamster ovary advancements in bioprocess regimens have led greatly increased product titres. The ability miRNAs regulate gene expression on a global level has generated considerable interest molecules as potential engineering targets. In this review, we briefly describe their organization...
Appears in: INTED2024 Proceedings Publication year: 2024Pages: 1304-1311ISBN: 978-84-09-59215-9ISSN: 2340-1079doi: 10.21125/inted.2024.0398Conference name: 18th International Technology, Education and Development ConferenceDates: 4-6 March, 2024Location: Valencia, Spain
Abstract RNA sequencing (RNASeq) has been widely used to associate alterations in Chinese hamster ovary (CHO) cell gene expression with bioprocess phenotypes, however alternative mRNA splicing, thus far, received little attention. In this study, we utilised RNASeq for transcriptomic analysis of a monoclonal antibody producing CHOK1 line subjected temperature shift. More than 2,365 instances differential splicing were observed 24hrs after the reduction culture temperature. 1,163 these events...
Abstract Chinese hamster ovary (CHO) cells are used to produce almost 90% of therapeutic monoclonal antibodies (mAbs). The annotation non-canonical translation events in these cellular factories remains incomplete, limiting not only our ability study CHO cell biology but also detect host protein (HCP) contaminants the final mAb drug product. We utilised ribosome footprint profiling (Ribo-seq) identify novel open reading frames (ORFs) including N-terminal extensions and thousands short ORFs...