A5087060289- Peptidase Inhibition and Analysis
- Enzyme Structure and Function
- Signaling Pathways in Disease
- Neuropeptides and Animal Physiology
- Enzyme Production and Characterization
- Insect Resistance and Genetics
- Entomopathogenic Microorganisms in Pest Control
- Biochemical and Molecular Research
- Protein Structure and Dynamics
- Ubiquitin and proteasome pathways
- Protease and Inhibitor Mechanisms
- Glycosylation and Glycoproteins Research
- Machine Learning in Bioinformatics
- Chemical Synthesis and Analysis
- Insect and Pesticide Research
- Pharmacogenetics and Drug Metabolism
- Genomics and Phylogenetic Studies
- Studies on Chitinases and Chitosanases
- Nonlinear Optical Materials Research
- RNA and protein synthesis mechanisms
- Advanced X-ray Imaging Techniques
- Structural and Chemical Analysis of Organic and Inorganic Compounds
- Phytochemicals and Medicinal Plants
- Phytochemistry and Bioactive Compounds
- Insect symbiosis and bacterial influences
Bhabha Atomic Research Centre
2014-2024
Indian Institute of Technology Indore
2024
Guru Kashi University
2023
Raja Ramanna Centre for Advanced Technology
2019-2022
Indian Institute of Toxicology Research
1990-2017
The protein crystallography beamline (PX-BL21), installed at the 1.5 T bending-magnet port Indian synchrotron (Indus-2), is now available to users. can be used for X-ray diffraction measurements on a single crystal of macromolecules such as proteins, nucleic acids and their complexes. PX-BL21 has working energy range 5-20 keV accessing absorption edges heavy elements commonly phasing. A double-crystal monochromator [Si(111) Si(220)] pair rhodium-coated mirrors are beam monochromatization...
The dichalcogenide ligated molecules in catalysis to produce molecular hydrogen through electroreduction of water are rarely explored. Here, a series heterometallic [Ag4(S2PFc(OR)4] [where Fc = Fe(η5-C5H4)(η5-C5H5), R Me, 1; Et, 2; nPr, 3; isoAmyl, 4] clusters were synthesized and characterized by IR, absorption spectroscopy, NMR (1H, 31P), electrospray ionization mass spectrometry. structures 1, 2, 3 established single-crystal X-ray crystallographic analysis. structural elucidation shows...
Serine peptidases of the prolyl oligopeptidase (POP) family are substantial therapeutic importance because their involvement in diseases such as diabetes, cancer, neurological diseases, and autoimmune disorders. Proper annotation knowledge substrate specificity mechanisms this highly valuable. Although endopeptidase, dipeptidyl peptidase, tripeptidyl acylaminoacyl peptidase activities have been reported previously, here we report first instance carboxypeptidase activity a POP member. We...
Acylaminoacyl peptidases (AAPs) play a pivotal role in various pathological conditions and are recognized as potential therapeutic targets. AAPs exhibit wide range of activities, such acylated amino acid-dependent aminopeptidase, endopeptidase, less studied carboxypeptidase activity. We have determined the crystal structure an AAP from Geobacillus stearothermophilus (S9gs) at 2.0 Å resolution. Despite being annotated aminopeptidase NCBI database, our enzymatic characterization proved S9gs to...
Xaa-Pro dipeptidase (XPD; prolidase; EC 3.4.13.9) specifically hydrolyzes dipeptides with a prolyl residue at the carboxy-terminus. Xanthomonas spp. possess two different isoforms of XPD (48 and 43 kDa) which share ∼24% sequence identity. The kDa in size (XPD43) from is unusual as it lacks strictly conserved tyrosine (equivalent to Tyr387 Escherichia coli aminopeptidase P) that suggested be important proton-shuttle transfer required for catalysis M24B (MEROPS) family. Here, crystallization...
Abstract M24B peptidases cleaving Xaa‐Pro bond in dipeptides are prolidases whereas those this longer peptides aminopeptidases‐P. Bacteria have small aminopeptidases‐P (36‐39 kDa), which diverged from canonical aminopeptidase‐P of Escherichia coli (50 kDa). Structure‐function studies lacking. We report crystal structures E. and Deinococcus radiodurans, substrate‐specificities these proteins their ortholog Mycobacterium tuberculosis. These aminopeptidases‐P, structurally close to except for...
LinA-type1 and LinA-type2 are two well-characterized variants of the enzyme 'hexachlorocyclohexane (HCH)-dehydrochlorinase'. They differ from each other at ten amino acid positions exhibit differing enantioselectivity for transformation (-) (+) enantiomers α-HCH. Amino acids responsible this enantioselectivity, however, not known. An in silico docking analysis identified four (K20, L96, A131, T133) that could be involved selective binding substrates. Experimental studies with constructed...
Peptidase-E, a nonclassical serine peptidase, is specific for dipeptides with an N-terminal aspartate. This stringent substrate specificity remains largely unexplained. We report aspartate-bound structure of peptidase-E at 1.83 Å resolution. In contrast to previous reports, the enzyme forms dimer, and active site located dimer interface, well shielded from solvent. Our findings further suggest that aspartate due electrostatics molecular complementarity in site. The new structural information...
Abstract Peptidase E (PepE) is a nonclassical serine peptidase with Ser‐His‐Glu catalytic triad. It specific for dipeptides an N‐terminal aspartate residue (Asp‐X dipeptidase activity). Its homolog from Listeria monocytogenes (PepElm) has Ser‐His‐Asn “catalytic triad.” Based on sequence alignment we predicted that the PepE Deinococcus radiodurans (PepEdr) would have Ser‐His‐Asp We confirmed this by solving crystal structure of PepEdr to 2.7 Å resolution. show PepElm and lack Asp‐X activity....
The causes of lung cancer might be many, but genetic variation in the genes carcinogen-metabolizing enzymes, tumor suppressor proteins, and/or DNA-repairing enzymes can also play a significant role susceptibility. protein p53 functions to induce cell cycle arrest, DNA repair, or apoptosis. Polymorphism its gene can, therefore, Present report evaluated association polymorphism exon 4 Arg72Pro (G>C) with susceptibility using 175 cases and 202 controls from North Indian population. Binary...
A bioinformatics-based protein-engineering approach called consensus design led to the construction of a chimeric triosephosphate isomerase (TIM) protein ccTIM (curated TIM) which is as active Saccharomyces cerevisiae TIM despite sharing only 58% sequence identity with it. The amino-acid this novel identical native sequences from eukaryotes those prokaryotes and shares some biophysical traits molten globular protein. Solving its crystal structure would help in understanding physical...
Acylpeptide hydrolase (APH; EC 3.4.19.1), which belongs to the S9 family of serine peptidases (MEROPS), catalyzes removal an N -acylated amino acid from a blocked peptide. The role this enzyme in mammalian cells has been suggested be clearance oxidatively damaged proteins as well degradation β-amyloid peptides implicated Alzheimer's disease. Detailed structural information for reported two thermophilic archaea; both archaeal APHs share similar monomeric structure. However, mechanisms...