A5081367802- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- RNA Interference and Gene Delivery
- SARS-CoV-2 detection and testing
- Advanced Biosensing Techniques and Applications
- Bacterial Genetics and Biotechnology
- Advanced Nanomaterials in Catalysis
- Advanced Fiber Optic Sensors
- Archaeology and ancient environmental studies
- Photonic and Optical Devices
- Gold and Silver Nanoparticles Synthesis and Applications
- Molecular Junctions and Nanostructures
- CRISPR and Genetic Engineering
- Metallurgy and Cultural Artifacts
- SARS-CoV-2 and COVID-19 Research
- Clostridium difficile and Clostridium perfringens research
- RNA modifications and cancer
- Analytical Chemistry and Sensors
- Electrochemical sensors and biosensors
- Electrochemical Analysis and Applications
- Chemical Synthesis and Analysis
- Optimal Experimental Design Methods
McMaster University
2016-2025
Jilin University
2025
Sichuan University
2018-2024
Tongji University
2024
University of South China
2024
Taiyuan Institute of Technology
2024
Institute of Archaeology
2023
Hamilton Health Sciences
2022
Yunnan University
2022
Kunming Medical University
2016-2021
Aptamers are single-stranded nucleic acids with defined tertiary structures for selective binding to target molecules. also able bind a complementary DNA sequence form duplex structure. In this report, we describe strategy designing aptamer-based fluorescent reporters that function by switching from DNA/DNA DNA/target complex. The is formed between fluorophore-labeled aptamer and small oligonucleotide modified quenching moiety (denoted QDNA). When the absent, binds QDNA, bringing fluorophore...
IMPORTANCE Clostridium difficile infection (CDI) is a major burden in health care and community settings.CDI recurrence of particular concern because limited treatment options associated clinical control issues.Fecal microbiota transplantation (FMT) promising, but not readily available, intervention.OBJECTIVE To determine whether frozen-and-thawed (frozen, experimental) FMT noninferior to fresh (standard) terms efficacy among patients with recurrent or refractory CDI assess the safety both...
A detailed understanding of the susceptibility RNA phosphodiesters to specific base-catalyzed cleavage is necessary approximate stability under various conditions. In addition, quantifying rate enhancements that can be produced exclusively by this common mechanism needed fully interpret mechanisms employed ribonucleases and RNA-cleaving ribozymes. Chimeric DNA/RNA oligonucleotides were used examine rates hydroxide-dependent degradation reaction conditions simulate those biological systems....
Picking out probes: A novel approach permits isolation of standard DNA aptamers by in vitro selection and conversion into fluorescence signaling probes. This method comprises the capable duplex-to-complex structure switching labeling derived with a pair short strands fluorophore F quencher Q to create reporter system for real-time sensing (see picture). Supporting information this article is available on WWW under http://www.wiley-vch.de/contents/jc_2002/2005/z461848_s.pdf or from author....
We have investigated the effect of folding DNA aptamers on colloidal stability gold nanoparticles (AuNPs) to which an aptamer is tethered. On basis studies two different (adenosine and K+ aptamer), we discovered a unique stabilization associated with folding: AuNPs folded structures are attached more stable toward salt-induced aggregation than those tethered unfolded aptamers. This significant when spacer was incorporated between AuNP or lower surface graft densities were used. The...
The majority of bioassays utilize thermosensitive reagents (e.g., biomolecules) and laboratory conditions for analysis. developing world, however, requires inexpensive, simple-to-perform tests that do not require refrigeration or access to highly trained technicians. To address this need, paper-based using gold nanoparticle (AuNP) colorimetric probes have been developed. In the two prototype DNase I adenosine-sensing assays, blue (or black)-colored DNA-cross-linked AuNP aggregates were...
ConspectusDNA-based enzymes, or DNAzymes, are not known to exist in Nature but can be isolated from random-sequence DNA pools using test tube selection techniques. Since the report of first DNAzyme 1994, many catalytic molecules for catalyzing wide-ranging chemical transformations have been and studied. Our laboratory has a keen interest searching diverse DNAzymes capable cleaving RNA-containing substrates, determining their sequence requirements structural properties, examining potential as...
Abstract Microbial pathogens pose serious threats to public health and safety, results in millions of illnesses deaths as well huge economic losses annually. Laborious expensive pathogen tests often represent a significant hindrance implementing effective front‐line preventative care, particularly resource‐limited regions. Thus, there is need develop low‐cost easy‐to‐use methods for detection. Herein, we present simple inexpensive litmus test bacterial The method takes advantage...
We report a simple and rapid saliva-based SARS-CoV-2 antigen test that utilizes newly developed dimeric DNA aptamer, denoted as DSA1N5, specifically recognizes the spike proteins of wildtype virus its Alpha Delta variants with dissociation constants 120, 290 480 pM, respectively, binds pseudotyped lentiviruses expressing alpha trimeric affinity 2.1 pM 2.3 respectively. To develop highly sensitive test, DSA1N5 was immobilized onto gold electrodes to produce an electrochemical impedance...
We performed in vitro selection experiments to identify DNA aptamers for the S1 subunit of SARS-CoV-2 spike protein (S1 protein). Using a pool pre-structured random sequences, we obtained over 100 candidate after 13 cycles enrichment under progressively more stringent pressure. The top 10 sequences all exhibited strong binding protein. Two aptamers, named MSA1 (Kd = 1.8 nM) and MSA5 2.7 nM), were assessed heat-treated protein, untreated spiked into 50% human saliva trimeric both wildtype...
Prompted by the increasing number of electrochemical biosensors reported in literature, a wide range lab-made potentiostats have been developed researchers recent years. While these devices are less costly than their commercial counterparts, they typically single-plex and rely on non-integrated sample preparation or signal actuation devices. To address limitations, we designed portable fully integrated platform for point-of-care (PoC) readout actuation. This device performs standard...
Abstract The development of aptamer technology considerably broadens the utility nucleic acids as molecular recognition elements, because it allows creation DNA or RNA molecules for binding a wide variety analytes (targets) with high affinity and specificity. Several recent studies have focused on developing rational design strategies transducing aptamer–target events into easily detectable signals, so that aptamers can be widely exploited detection directed applications. We devised...
DNA sequences were isolated by in vitro selection for binding to N-methylmesoporphyrin IX (NMM), a molecule that behaves as stable transition-state analogue porphyrin chelatases. Clones ∼280 and ∼120 nucleotides long obtained, which bound NMM with sub-micromolar affinity but mesoporphyrin (MPIX), well various metalloderivatives of MPIX, lower affinity. Footprinting experiments dimethyl sulfate, DNase I, hemin molecules activated superoxide identified series short guanine-rich motifs be the...