A5102808818- Enzyme-mediated dye degradation
- Fungal Biology and Applications
- Microbial Metabolism and Applications
- Plant Pathogens and Fungal Diseases
- Fungal and yeast genetics research
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Biofuel production and bioconversion
- Yeasts and Rust Fungi Studies
- Plant and fungal interactions
- Mycorrhizal Fungi and Plant Interactions
- Chromium effects and bioremediation
- Insect Resistance and Genetics
- Metalloenzymes and iron-sulfur proteins
- Biochemical and biochemical processes
- Lignin and Wood Chemistry
- CRISPR and Genetic Engineering
- Polysaccharides and Plant Cell Walls
- Fermentation and Sensory Analysis
- Algal biology and biofuel production
- Microbial Natural Products and Biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Insect symbiosis and bacterial influences
- Infectious Diseases and Mycology
- Turfgrass Adaptation and Management
University of Shiga Prefecture
2014-2024
Iwate Biotechnology Research Center
2003-2017
Kyoto University
1996-2001
GTx (United States)
2000
Kyoto Bunkyo University
1998
Kagawa University
1995
Abstract In a previous study, we reported transient transformation system using repeated screening for hygromycin B (Hyg) resistance in the basidiomycete Ceriporiopsis subvermispora . present by combining this technique with CRISPR/Cas9, demonstrated successful marker-free genome editing Pleurotus ostreatus , which is one of most economically important cultivated mushrooms as well model white-rot fungus. At first, transformant selection mediated expression marker genes was plasmid harboring...
A genetic transformation system was developed for the selective white rot basidiomycete Ceriporiopsis subvermispora using a modified protocol with polyethylene glycol and CaCl2 treatment of protoplasts plasmids harboring recombinant hygromycin phosphotransferase (hph) driven by homologous promoter. During repeated transfer on fresh potato dextrose agar plates containing 100 µg/ml B, most transformants lost drug resistance, while remaining isolates showed stable resistance over five...
Abstract First, we attempted to recombine the Shiitake (Lentinula edodes) pyrG (ura3) gene homologously by introducing a donor vector containing carboxin resistance (lecbxR) flanked homologous sequences of into protoplasts fungus. However, all carboxin-resistant transformants only contained ectopic insertions exogenous and no insertions. Agaricomycetes are generally known for their low efficiency recombination, similar result was shown L. edodes. We then co-introduced Cas9 plasmid...
We cloned a gene for the iron sulfur protein (Ip) subunit from an edible mushroom, Lentinula edodes, and introduced point mutation that confers carboxin resistance into it. The mutant successfully transformed L. edodes with high efficiency (9 transformants/2.5 microg vector DNA). Restriction enzyme-mediated integration (REMI) increased transformation by about two-fold.
We constructed a LongSAGE (Long Serial Analysis of Gene Expression) library from 3-d culture Phanerochaete chrysosporium supplemented with atropine, which inhibits the production lignin-degrading enzymes. The (the atropine library) contains 13,108 tags and 6,783 unique tags. gene expression profile represented by was compared those two previously libraries, one using 2-d cultures in fungus had not yet produced ligninolytic enzymes other just started to produce library). found total 595 genes...
We studied the role of genes encoding cAMP-dependent protein kinase A catalytic subunit (PKAc) in ligninolytic system Pleurotus ostreatus. The wild-type P. ostreatus strain PC9 has two PKAc-encoding genes: PKAc1 and PKAc2 (protein ID 114122 85056). In current study, were fused with a β-tubulin promoter introduced into to produce overexpression strains PKAc1-97 PKAc2-69. These showed significantly higher transcription levels isozyme lignin-modifying enzymes than PC9, but specific gene...
The capacity of white-rot fungi to degrade wood lignin may be highly applicable the development novel bioreactor systems, but mechanisms underlying this function are not yet fully understood. Lignin peroxidase (LiP) and manganese (MnP), which thought very important for ligninolytic property, demonstrated increased activity in Phanerochaete chrysosporium RP-78 (FGSC #9002, ATCC MYA-4764™) cultures following exposure 5 mM cyclic adenosine 3', 5'-monophosphate (cAMP) 500 μM...
Transformation of Pleurotus ostreatus PC9 with the mutated heterotrimeric G protein alpha subunit (Gα) gene resulted in higher laccase (Lac) activity and intracellular cyclic adenosine monophosphate (cAMP) concentrations as compared to those wild-type PC9. The transformant also exhibited Lac than wild type when cultured a medium containing known inducers CuSO4 ferulic acid.
Choke diseases were surveyed in two closely related grass species, Agropyron ciliare var. minus and tsukushiense transiens, Shiga Prefecture, Japan. Perithecia ascospores not observed either case. Stromata on A. enclosed sterilized young inflorescences, as the typical choke symptoms by Epichloë typhina. On other hand stromata tsukusiense transiens thinly covered mature spikes with white epiphyllous hyphae, of Ephelis spp. The fungal isolates produced Neotyphodium-type conidia. Molecular...