Salmonella spp. are considered the main agents of foodborne disease and Enteritidis is one most frequently isolated serovars worldwide. The virulence their interaction with host complex processes involving factors to overcome defenses. purpose this study was detect genes in S. isolates from poultry South Brazil. PCR-based assays were developed order nine (lpfA, agfA, sefA, invA, hilA, avrA, sopE, sivH spvC) associated eighty-four poultry. sivH, sefA avrA present 100% isolates; lpfA sopE 99%;...

ABSTRACT: Salmonella spp. are one of the most important agents foodborne disease in several countries, including Brazil. Poultry-derived products common food products, meat and eggs, involved outbreaks human salmonellosis. has capacity to form biofilms on both biotic abiotic surfaces. The biofilm formation process depends an interaction among bacterial cells, attachment surface environmental conditions. These structures favor survival hostile environments, such as slaughterhouses processing...

Pasteurella multocida causes atrophic rhinitis in swine and fowl cholera birds, is a secondary agent respiratory syndromes. Pathogenesis virulence factors involved are still poorly understood. The aim of this study was to detect 22 virulence-associated genes by PCR, including capsular serogroups A, B D evaluate the antimicrobial susceptibility P. strains from poultry swine. ompH, oma87, plpB, psl, exbD-tonB, fur, hgbA, nanB, sodA, sodC, ptfA were detected more than 90% both hosts. 91% 92%...

During the last years, Brazilian government control programs have detected an increase of Salmonella Heidelberg in poultry slaughterhouses a condition that poses threat to human health However, reasons remain unclear. Differences genetic virulence profiles may be possible justification. In addition, effective is related efficient epidemiological surveillance system through genotyping techniques. this context, aim study was detection 24 virulence-associated genes 126 S. isolates. We...

Eighty Salmonella Enteritidis strains isolated from broiler carcasses between May 1995 and April 1996 in the State of Rio Grande do Sul, Brazil, were tested for antibiotic susceptibility using disk diffusion method. Resistance to colistin, novobiocin, erythromycin tetracycline was observed 100% isolates. The showed intermediate resistance at different levels kanamycin (1.25%), enrofloxacin (3.75%), neomycin fosfomycin (20%), sulphonamides (86.25%) nitrofurantoin (90%). ciprofloxacin,...

Salmonella spp. remain among the most important agents of foodborne diseases worldwide. The importance in public health is linked to their wide range antimicrobial resistance and pathogenicity virulence both human animal hosts. aim this study was determine patterns for serotypes isolated from poultry sources Brazil detect virulence-associated genes verify association with specific serotypes. A total 163 strains enterica Southern were selected, each belonged one 11 different They tested...

Salmonella spp. are one of the most common causes foodborne disease outbreaks worldwide. Disinfectants widely used in food industry to reduce pathogen contamination, but increase antimicrobial resistance has reinforced global need for effective and environmentally friendly alternatives. In addition, Heidelberg, an emergent serotype, been described as highly persistent facilities poultry production chain. this context, present study aimed evaluate action electrochemically activated water...

This study assessed biofilm formation on polystyrene by Staphylococcus aureus, Listeria monocytogenes, L. welshimeri and Escherichia coli, isolated from a slaughtering plant, grown tryptic soy broth (TSB) using different glucose concentrations. The tested bacteria produced in at least one of the concentrations used, some them were strong producers.

Abstract Campylobacter jejuni is one of the most common causes foodborne diseases worldwide. There are few reports on strains isolated from Latin-American countries. Here, 140 C. cloacal and transport boxes swabs, water chiller tanks, broiler carcasses five poultry companies in Southern Brazil were identified using phenotypic genotypic methods. Polymerase chain reaction (PCR) was used to analyze eight virulence markers: fla A, cad F, invasion-associated ( iam ) genes, cdt ABC operon...

Abstract 1. Ultrastructural analyses of the eggshells one strain commercial layers indicated that cuticular layer is rarely present as an even covering over shell surface and paired membranes are invariably pitted with holes larger than bacterial dimensions. 2. The above conditions pertain irrespective age laying bird. 3. Bacterial penetration eggshell independent pore numbers. 4. In absence a functional cuticle removed, bacteria checked in their movement by structural modifications mammillary layer.

The present study was carried out to evaluate the occurrence of Salmonellae in raw broiler parts and determine antimicrobial resistance profile isolated strains. Twenty-four (39.3%) samples were positive for Salmonella twenty-five strains isolated, since two different serovars detected one single sample. Enteritidis most prevalent serovar. Among isolates, 95.2% belonged Phage Type 4 (PT4) (20/21) 4.8% PT7 (1/21). Twenty-two (88%) resistant at least agent, generating eight patterns. S....

Salmonella Heidelberg is one of the 3 most frequently isolated serovars from human cases in Canada, and fourth commonly reported serovar foodborne disease United States. Since 1962, has been poultry products Brazil. The industry focused efforts on reducing salmonellae incidence live production an effort to reduce processing plant. A better understanding initial infection chicks could provide approaches control contamination. objective present study was evaluate 2 strains that differed...

Over the years, Salmonella Heidelberg (SH) has gained prominence in North America poultry production and of other countries. been isolated reported from products Brazil since 1962, whereas Enteritidis (SE) only emerged as a serious problem public health 1993. These strains can cause intestinal problems newly hatched chicks, infection may persist until adulthood. Upon slaughter chickens, contaminate carcasses, condition that poses threat to human health. The aim this study was compare fecal...

Bactérias do gênero Salmonella são consideradas a principal causa de infecções alimentares em humanos associadas produtos avícolas, na maioria dos países. São capazes formar biofilmes diferentes superfícies, dificultando sua eliminação por procedimentos limpeza e sanificação indústria alimentos. Estudos demonstram uma melhor adesão microbiana com o aumento da hidrofobicidade, tanto superfície celular como substrato adesão. Neste trabalho, avaliou-se formação biofilme placas poliestireno S....

Salmonellosis ranks among the major diseases of commercial poultry, and its presence in poultry flocks is responsible for economic losses risks related to public health. Vaccines are an important tool within integrated programmes control salmonellosis. The purpose this study was assess cross-protection provided by Poulvac® ST vaccine Salmonella Heidelberg experimentally challenged 3- 21-day-old birds. Eighty birds were identified separated into four treatments (T1: vaccinated at 3 days age,...

To assess diversity of Salmonella enterica serotypes present in poultry and their environment from southern Brazil, the Kauffmann-White-Le Minor (KWL) scheme was used to serotype a total 155 isolates. Isolates were then re-examined with nested PCR sequencing dkgB-linked intergenic sequence ribotyping (ISR) region that assesses single nucleotide polymorphisms occurring around 5S ribosomal gene. Serotypes identified Heidelberg (40·6%), Enteritidis (34·2%), Hadar (8·4%), Typhimurium (3·9%),...

The objective of this study was to determine fluoroquinolone resistance in Campylobacter spp from poultry and human isolates. Forty-one jejuni isolates (30 origin 11 origin) coli (10 1 were examined for ciprofloxacin, norfloxacin, nalidixic acid using the minimal inhibitory concentration (MIC) method. Thereafter, analyzed by PCR–Restriction Fragment Length Polymorphism (RFLP) assay detection Thr-86 mutation. Finally, DNA sequencing performed confirmation gyrA gene A complete correlation...

1. Epidemiological surveillance of Salmonella spp. serves as a primary tool for maintaining the health poultry flocks. Characterising circulating serotypes is crucial implementing control and prevention measures. This study conducted phenotypic molecular characterisation S. enterica Pullorum, Heidelberg, Corvalis isolated from broiler chickens during slaughtering.