A5102972811- Central Venous Catheters and Hemodialysis
- Vascular Malformations Diagnosis and Treatment
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Vascular anomalies and interventions
- Vascular Procedures and Complications
- Plant Virus Research Studies
- Coronary Interventions and Diagnostics
- Intracranial Aneurysms: Treatment and Complications
- Signaling Pathways in Disease
- Angiogenesis and VEGF in Cancer
- Cardiac and Coronary Surgery Techniques
- RNA regulation and disease
- Vector-Borne Animal Diseases
- Aortic aneurysm repair treatments
- Congenital heart defects research
- Genetics and Neurodevelopmental Disorders
- Mesenchymal stem cell research
- Fibroblast Growth Factor Research
- T-cell and Retrovirus Studies
- Aortic Disease and Treatment Approaches
- Herpesvirus Infections and Treatments
- Wound Healing and Treatments
- Vascular Malformations and Hemangiomas
- Venous Thromboembolism Diagnosis and Management
Yale University
2005-2024
First Affiliated Hospital of Zhengzhou University
2017
VA Connecticut Healthcare System
2017
Zhengzhou University
2017
University of Alberta
2008
State University of New York
2002
Institut Curie
1989-1996
Centre National de la Recherche Scientifique
1996
Signal transduction through epidermal growth factor receptors (EGFRs) is essential for the and development of multicellular organisms. A genetic screen regulators EGFR signaling has led to identification Sprouty, a cell autonomous inhibitor EGF that transcriptionally induced by pathway. However, molecular mechanisms which Sprouty exerts its antagonistic effect remain largely unknown. Here we have used transient expression in human cells investigate functional properties (hSpry) proteins....
Arteriovenous fistulae (AVF) are the most common access created for hemodialysis, but up to 60% do not sustain dialysis within a year, suggesting need improve AVF maturation and patency. In mouse model, Akt1 regulates fistula wall thickness diameter. We hypothesized that inhibition of Akt1-mTORC1 axis alters venous remodeling Daily intraperitoneal injections rapamycin reduced with no change in Rapamycin decreased smooth muscle cell (SMC) macrophage proliferation; also both M1 M2 type...
Arteriovenous fistulae (AVF) are the gold standard for vascular access hemodialysis. Although vein must thicken and dilate successful hemodialysis, excessive wall thickness leads to stenosis causing AVF failure. Since TGF-β (transforming growth factor-beta) regulates ECM (extracellular matrix) deposition smooth muscle cell (SMC) proliferation-critical components of thickness-we hypothesized that disruption signaling prevents thickening during venous remodeling.A mouse aortocaval fistula...
After arteriovenous fistula creation, females have lower rates of maturation and higher failure than males. In a mouse model venous adaptation that recapitulates human maturation, sex hormones may be mechanisms the sexual dimorphism: testosterone is associated with reduced shear stress, whereas estrogen increased immune cell recruitment. Modulating or downstream effectors suggests sex-specific therapies could address disparities in differences clinical outcomes.
Clinical failure of arteriovenous neointimal hyperplasia (NIH) fistulae (AVF) is frequently due to juxta-anastomotic NIH (JANIH). Although the mouse AVF model recapitulates human maturation, previous studies focused on outflow vein distal anastomosis. We hypothesized that area (JAA) has increased compared with vein. was created in C57BL/6 mice without or chronic kidney disease (CKD). Temporal and spatial changes JAA were examined using histology immunofluorescence. Computational techniques...
The leukemia-associated protein EVI1 possesses seven zinc fingers within an N-terminal domain (amino acids 1–250) that binds to GACAAGATA. Single amino acid missense mutants of were developed failed bind DNA either in vitro, as assessed by gel shift assay, or vivo, shown transactivation studies. Specifically, mutation R205N lacks high affinity binding the GACAAGATA motif. Putative target genes identified using EVI1-(1–250)-VP16 fusion acts a transcriptional activator with specificity EVI1....
Abstract Polyester is commonly used in vascular surgery for patch angioplasty and grafts. We hypothesized that polyester patches heal by infiltration of arterial or venous progenitor cells depending on the site implantation. were implanted into Wistar rat aorta inferior vena cava explanted day 7 30. Neointima formed was thicker environment compared to amount environment. Venous had more cell proliferation greater numbers VCAM‐positive CD68‐positive cells, whereas vimentin‐positive...
The arteriovenous fistula (AVF) is the preferred method of dialysis access because its proven superior long-term outcomes.However, women havelower rates AVF patency andutilizationthan men.We used a novel mouseAVF model that recapitulates human maturation to determine whether there are differences in female and male mice.Aortocaval fistulas were created C57BL/6 mice (9-10 weeks). At days 0, 3, 7, 21, infrarenal inferior vena cava (IVC) aortic diameters flow velocity monitored by Doppler...
Pseudoaneurysms remain a significant complication after vascular procedures. We hypothesized that TGF-β (transforming growth factor-β) signaling plays mechanistic role in the development of pseudoaneurysms.Rat aortic pericardial patch angioplasty was associated with high incidence (88%) pseudoaneurysms at 30 days, increased smad2 phosphorylation small but not large pseudoaneurysms; TGF-β1 receptors were and preserved pseudoaneurysms. Delivery via nanoparticles covalently bonded to stimulated...
Objective— Arteriovenous fistulae (AVF) are the most common access created for hemodialysis; however, many AVF fail to mature and require repeated intervention, suggesting a need improve maturation. Eph-B4 (ephrin type-B receptor 4) is embryonic venous determinant that functional in adult veins can regulate Cav-1 (caveolin-1) major scaffolding protein of caveolae—a distinct microdomain serves as mechanosensor at endothelial cell membrane. We hypothesized function critical Eph-B4–mediated...
Objective: Arteriovenous fistulae (AVF) are the optimal conduit for hemodialysis access but have high rates of primary maturation failure. Successful AVF requires wall thickening with deposition ECM (extracellular matrix) including collagen and fibronectin, as well lumen dilation. TAK1 (TGFβ [transforming growth factor-beta]–activated kinase 1) is a mediator noncanonical TGFβ signaling plays crucial roles in regulation production deposition; therefore, we hypothesized that regulates dilation...
Calreticulin is a Ca (2+)-buffering chaperone of the endoplasmic reticulum. The protein highly expressed in embryonic heart but downregulated postnatal heart, indicating that expression calreticulin regulated. In this study we identify GATA6 and Evi-1 transcription factors as new regulators gene. neonatal rat ventricular cardiomyocytes mouse fibroblasts gene activated by repressed Evi-1. Furthermore, transactivation suppressed Evi-1, suggesting an antagonistic role between both Using EMSA,...
When injected into chickens, a transformation-defective mutant of the Prague C strain Rous sarcoma virus induced tumors at low incidence and after long latency. One such tumor released replication-defective designated PR2257. We molecularly cloned sequenced proviral DNA from quail fibroblasts transformed by Comparison PR2257 sequence with that C, cellular src, 3' adjacent showed spliced version c-src gene about 950 base pairs (bp) 3'-flanking were transduced This transduction eliminated...
Avian sarcoma virus PR2257 transduced de novo the c- src gene and about 900 bp of 3‣ non-coding sequences belonging to locus. This contains only one mutation in c-src coding sequence causing a reading frame shift after Pro-525. The molecular clone studied was derived from cell line transformed quail fibroblasts, Cl. It endogenous (ev) U5 regions, indicating that multiple recombination occurred with virus. Here we investigated possible evolution when original tumour repeatedly passaged vivo....
Clinical outcomes of arteriovenous fistulae (AVF) for hemodialysis remain inadequate since biological mechanisms AVF maturation and failure are still poorly understood. Aortocaval fistula creation (AVF group) or a sham operation (sham was performed in C57BL/6 mice. Venous limbs were collected on postoperative day 7 total RNA extracted high throughput sequencing bioinformatic analysis. Genes metabolic pathways significantly downregulated the AVF, whereas significant sex differences not...
Objectives: Arteriovenous fistulae (AVF) are the preferred vascular access for patients with end-stage renal disease (ESRD) but have high rates of primary failure. Models AVF using mice chronic kidney (CKD) show diminished maturation and increased failure compared to wild type mice. Since CKD TGF-β1 expression activity in model, signaling can alter autophagy, we hypothesized that increases autophagy during maturation, whereas inhibition smooth muscle cell (SMC) TGF-β receptor would attenuate...
Central venous stenosis (CVS) is a major cause of arteriovenous fistula (AVF) failure. However, central veins are relatively inaccessible to study with conventional Doppler ultrasound methods. To understand mechanisms underlying AVF failure owing CVS, an animal model was established that creates distal AVF. We hypothesized this mouse will show comparable morphology and physiology human CVS.An aortocaval created between the aorta inferior vena cava (IVC); then by partial IVC ligation....
Вирусы и клетка
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