A5090659492- Genetically Modified Organisms Research
- CRISPR and Genetic Engineering
- Pesticide Residue Analysis and Safety
- Food Allergy and Anaphylaxis Research
- Animal Genetics and Reproduction
- Analytical Chemistry and Chromatography
- Esophageal and GI Pathology
- Analytical chemistry methods development
- Mycotoxins in Agriculture and Food
- Allergic Rhinitis and Sensitization
- Esophageal Cancer Research and Treatment
- Insect Resistance and Genetics
- Food Quality and Safety Studies
- Identification and Quantification in Food
- Dye analysis and toxicity
- Transgenic Plants and Applications
- Contact Dermatitis and Allergies
- Consumer Attitudes and Food Labeling
- Mercury impact and mitigation studies
- Gastric Cancer Management and Outcomes
- Phytochemicals and Antioxidant Activities
- Heavy Metal Exposure and Toxicity
- Tracheal and airway disorders
- Effects and risks of endocrine disrupting chemicals
- Radioactive contamination and transfer
Hoshi University
2021-2024
National Institute of Health Sciences
2015-2024
Ebara (Japan)
2021-2024
Waseda University
2022
Kawasaki Hospital
2021
Enoshima Aquarium
2021
Osaka University
2016-2019
Hiratsuka City Hospital
2018
High Pressure Gas Safety Institute of Japan
2015
The University of Tokyo
1972-2014
In this study, we investigated the absorption of apple procyanidins, namely, condensed tannins (ACTs), in rats using Porter method and high-performance liquid chromatography/tandem mass spectrometry. The procyanidin concentrations rat plasma reached a maximum 2 h after administration decreased thereafter. To investigate limits procyanidins polymerization degree, administered oligomer fraction, which was separated from ACT normal-phase chromatography according to degree polymerization....
Esophagoplasty with isoperistaltic stomach is a useful and satisfactory means of reconstruction the esophagus. It simpler there no necessity for using reversed gastric tube. The most essential points technique modelling stomach, taking advantage ample intramural vascular network adequate selection highest point site esophago-or pharyngogastrostomy. To utilize network, sufficient width necessary. This also allows stretching stomach. Esophageal resection above level neck was performed on 130...
An inter-laboratory evaluation study was conducted in order to evaluate the antioxidant capacity of food additives by using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Four antioxidants used as existing (i.e., tea extract, grape seed enju and d-α-tocopherol) 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) were analytical samples, 14 laboratories participated this study. The repeatability relative standard deviation (RSDr) IC50 Trolox, four antioxidants, Trolox equivalent...
Abstract This document provides supplemental guidance on specifications for the development and implementation of studies to validate performance characteristics quantitative ELISA methods determination food allergens. It is intended as a companion other existing publications method validation. The divided into two sections: information be provided by developer various method, multilaboratory validation study. Certain criteria included in are allergen-specific. Two allergens, egg milk, used...
Rapid analysis of multiple food allergens is required to confirm the appropriateness allergen labelling in processed foods. This study aimed develop a rapid and reliable method simultaneously detect trace amounts seven allergenic proteins (wheat, buckwheat, milk, egg, crustacean, peanut, walnut) foods using LC-MS/MS. Suspension-trapping (S-Trap) columns on-line automated solid-phase extraction were used improve complex time-consuming pretreatment process previously for The developed enabled...
Seven lines of genetically modified (GM) maize have been authorized in Japan as foods and feeds imported from the USA. We improved a multiplex PCR method described previous report order to distinguish five GM maize. Genomic DNA was extracted with silica spin column kit, which could reduce experimental time improve safety laboratory potentially environment. sequenced recombinant (r-DNA) introduced into maize, re-designed new primer pairs increase specificity by PCR. A pair for intrinsic zein...
Abstract In July 2018, certain valsartan-containing drugs were voluntary recalled in Japan owing to contamination with N -nitrosodimethylamine (NDMA), a probable human carcinogen. this study, an HPLC method was developed for the quantitative detection of NDMA simultaneously eluted valsartan. Good linearity correlation coefficient (R 2 ) > 0.999 achieved over concentration range 0.011–7.4 µg/mL. The limits and quantification 0.0085 μg/mL 0.0285 μg/mL, respectively. When valsartan samples...
Abstract Novel analytical methods based on real-time quantitative polymerase chain reactions by use of new reference molecules were validated in interlaboratory studies for the quantitation genetically modified (GM) maize and soy. More than 13 laboratories from Japan, Korea, United States participated studies. The included 2 separate stages: (1) measurement tests coefficient values, ratio recombinant DNA (r-DNA) sequence, endogenous sequence seeds GM soy; (2) blind with 6 pairs soy samples,...
The incidence of type I allergic disorders has been increasing worldwide, particularly, the hypersensitivity to food. We first showed that apple condensed tannin (ACT) intake would inhibit development oral sensitization and inhibition could correlate with rise in population TCRγδ‐T cells intestinal intraepithelial lymphocytes (IEL) using W/W V mice B10A which were ovalbumin (OVA)‐orally sensitized. Serum OVA‐specific immunoglobulin E G1 titers OVA‐orally sensitized ad libitium fed ACT...
The Japanese food allergen labeling regulation was designed to match real allergy circumstances and also be enforced effectively; thus, (1) regulated allergens were selected by prevalence seriousness according surveys in Japan; (2) the detection criterion for ELISA monitoring, 10 μg protein/g (or mL) food, set up as threshold value regulate commercial prepackaged foods; (3) official analytical methods, which can determine accurately, developed. These three points are distinctive from other...
Polymerase Chain Reaction (PCR) techniques are increasingly used for the detection of genetically modified (GM) crops in foods. In this paper, recombinant DNAs introduced into seven lines GM maize, such as Event 176, Bt11, T25, MON810, GA21, DLL25, and MON802, sequenced. On basis obtained sequence, 14 primer pairs segments, promoter, terminator regions, construct genes, were designed. To confirm specificities designed pairs, PCR was performed on genomic extracted from non-GM soy, other...
In this study, we developed a novel multiplex polymerase chain reaction (PCR) method for simultaneous detection of up to eight events genetically modified (GM) maize within single reaction. The primer pairs designed be construct specific respective GM (i.e., Bt11, Event176, GA21, MON810, MON863, NK603, T25, and TC1507) pair an endogenous reference gene, ssIIb, were included in the nonaplex(9plex) PCR system, its amplified products could distinguished by agarose gel capillary electrophoreses...
We developed a novel type of real-time polymerase chain reaction (PCR) array with TaqMan chemistry as platform for the comprehensive and semiquantitative detection genetically modified (GM) crops. Thirty primer-probe sets specific GM lines, recombinant DNA (r-DNA) segments, endogenous reference genes, donor organisms were synthesized, 96-well PCR plate was prepared different in each well array. The specificity sensitivity evaluated. A comparative analysis data publicly available information...
2nd Department of Surgery and Radiology, Faculty Medicine, University Tokyo, Japan *Present address: Dept. Thoracic Surg., Tokyo Metropolitan Police Hospital, 2–10-41, Fujimi, Chiyoda ku, **Dept. Univ. Tokyo.
Cochineal dye is used worldwide as a red coloring in foods, drinks, cosmetics, quasi-drugs, and drugs. The main component of the color carminic acid (CA). Carmine an aluminum- or calcium-chelated product CA. CA carmine usually contain contaminating proteins, including 38-kDa protein thought to be primary allergen. Severe allergic reactions manifest anaphylaxis. aim this study was review all Japanese reported cases propose useful diagnostic chart.All cochineal dye-induced immediate allergy...
In the Japanese allergy-labeling system, food labeling is mandated for 7 specific ingredients (egg, cow's milk, wheat, buckwheat, peanut, shrimp, and crab) recommended 21 in reference to case numbers of actual illness degree seriousness. To monitor validity official methods detection ingredient proteins processed foods were developed. The consist ELISA screening, western blot egg PCR shrimp/prawn, crab as confirmation tests. Threshold amounts (a few mg/kg) set based on approach analytical...
We developed a simple and reliable analytical method using high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) to simultaneously detect walnut almond as specified in regulations for food allergen labelling processed foods. Five specific target peptides derived from 2S albumin 7S globulin three 11S were selected by analysing several varieties of almond, eight kinds other nuts, ten major ingredients or cereals. The limit detection the peptide GEEMEEMVQSAR (m/z 698.3...