Bacillus spp. produce a broad spectrum of lipopeptide biosurfactants, among which surfactin, iturin and fengycin are widely studied families. The goals this study were to characterize the biosurfactant activity investigate their motility biofilm formation capabilities. In addition, we extracted lipopeptides from these bacteria assess antifungal activities analyzed products by mass spectrometry (MS). B. amyloliquefaciens FZB42, sp. NH 217 subtilis NH-100 exhibited excellent surface spreading...

In the surroundings of a former Pb/Zn smelter in Arnoldstein (Austria) heavy metal concentrations planted crops exceed thresholds for usage as food and feed. The aim was to study effects plant growth-promoting bacterial strain combination with immobilizing soil amendments on growth, uptake microbial community structure. Pot experiments were performed whereby two maize cultivars grown different contaminated soils treatments consisted Burkholderia phytofirmans PsJN without addition gravel...

Laboratory and greenhouse experiments have shown that root-associated bacteria beneficial effects on grapevine growth; however, these not been tested in the field. Here, we aimed to demonstrate whether of different geographical origins derived from crop plants can colonize gain a outcome for plant leading promote growth at field scale. To link ecological functions promotion growth, sorted fifteen bacterial strains larger isolate collection study vitro Plant Growth Promoting (PGP) traits. We...

Microbacterium species have been isolated from a wide range of hosts and environments, including heavy metal-contaminated sites. Here, we present comprehensive analysis on the phylogenetic distribution genetic potential 70 belonging to 20 different non-contaminated sites with particular attention secondary metabolites gene clusters. The analyzed are divided in three main functional clades. They share small core genome (331 families covering basic functions) pointing high diversity. most...

Abstract Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) is an invasive pest for agricultural production. It associated with the primary symbiont “ Candidatus Pantoea carbekii”, which essential host's fitness. In a laboratory rearing, significant loss of fitness was observed, but reasons remained unknown. Since bacterial symbionts are known to have important impact on their hosts, we investigated gut microbiota laboratory‐reared H. individuals based 16S rRNA gene metabarcoding. We...

Streptomycetes, typical soil dwellers, can be detected as common colonizers of human bodies, especially the skin, respiratory tract, guts and genital tract using molecular techniques. However, their clinical manifestations isolations are rare. Recently they were discussed possible "coaches" immune system in connection with certain disorders cancer. This work aimed for characterization evaluation genetic adaptations a human-associated strain Streptomyces sp. TR1341. The was isolated from...

Wolbachia is a maternally inherited obligate endosymbiont that can induce wide spectrum of effects in its host, ranging from mutualism to reproductive parasitism. At the genomic level, recombination within and between strains, transposable elements, horizontal transfer strains host species make an evolutionarily dynamic bacterial system. The invasive cherry fruit fly Rhagoletis cingulata arrived Europe North America ~40 years ago, where it now co-occurs with native pest R. cerasi. This...

Heritable beneficial bacterial endosymbionts have been crucial for the evolutionary success of numerous insects by enabling exploitation nutritionally limited food sources. Herein, we describe a previously unknown dual endosymbiosis in psyllid genus

Microbacterium spp. isolated from heavy metal (HM)-contaminated environments (soil and plants) can play a role in mobilization processes the phytoextraction of HM. Here, we report whole-genome sequences annotation 10 both HM-contaminated -noncontaminated compartments.

Abstract Agromyces aureus AR33 T is a Gram-positive, rod-shaped and motile bacterium belonging to the Microbacteriaceae family in phylum Actinobacteria that was isolated from former zinc/lead mining processing site Austria. In this study, whole genome sequenced assembled combining sequences obtained Illumina MiSeq Sanger sequencing. The assembly resulted complete sequence which 4,373,124 bp long has GC content of 70.1%. Furthermore, we performed comparative genomic analysis with other...

A Gram-reaction-positive, motile, yellow-pigmented and rod-shaped bacterial strain, designated AR33T, was isolated from the rhizosphere of Salix caprea L. growing in a former zinc/lead mining processing site Austria. polyphasic approach applied to determine its taxonomic position. 16S rRNA gene sequence analysis, morphological chemotaxonomic properties showed that strain AR33T belongs genus Agromyces. Strain had peptidoglycan type B2γ major menaquinones were MK-11, MK-10 MK-12. The main...

Abstract Wolbachia is one of the most abundant intracellular symbionts arthropods and has profound effects on host biology. transmission phenotypes often depend its density within host, which can be affected by multiple biotic abiotic factors. However, very few studies measured in natural populations. Here, we describe pear psyllid Cacopsylla pyri from three populations Czech Republic. Using phylogenetic analyses based wsp multilocus sequence typing genes, demonstrate that C. harbours new...

Abstract The European spruce bark beetle Ips typographus is a pest causing severe damages in forests dominated by the Norway Europe. Microorganisms play an essential role host species performance, including nutrition, fitness as well overcoming defenses. Here, we performed high-throughput 16S rRNA metabarcoding of I. across different populations Europe, to assess its bacterial community. We investigated four postglacial refugial areas Europe and focused specifically on current hot spot...

Phytoplasmas are bacterial pathogens located in the plant's phloem that responsible for several plant diseases and mainly transmitted by phloem-sucking insects. Apple proliferation (AP) is an economically important disease associated with presence of '

<title>Abstract</title> The Brown marmorated stink bug <italic>Halyomorpha halys </italic>is one of the most harmful invasive species worldwide. It is highly polyphagous with ability to feed on more than 300 different plants. So far, studies describe host plants based visual observations, which allowed diagnose important in range North America and Europe. However, exact feeding behavior this pest not known. By applying a molecular gut content analysis approach individuals collected field...

Abstract The relationship between phytophagous insects and plants is a central aspect of food webs ecosystem functioning. introduction new species into an environment can have significant impacts on the web native ecosystem. In many cases, there lack knowledge biology feeding behavior invasive prior their in invaded regions. Gut content analyses provided valuable information host spectrum insects. However, current approaches are time‐consuming costly. Here, we describe molecular gut analysis...

The gut microbiome plays an important role in insect evolution and ecology. Bacteria support the host's nutrition defense therefore play fitness of host. Halyomorpha halys is one most invasive pest species world. Native to North-Eastern Asia, this Pentatomid bug has recently invaded North America Europe, causing significant damage agricultural production. Although increasing number studies investigated biology species, little known about composition its microbiota. Like many other H. harbors...

Notwithstanding the fact that streptomycetes are overlooked in clinical laboratories, studies describing their occurrence disease and potential pathogenicity emerging. Information on species diversity specimens, aetiology appropriate therapeutic treatment is scarce. We identified evaluated antibiotic susceptibility profile of 84 Streptomyces isolates from Czech Republic. In absence disk diffusion (DD) breakpoints for testing (AST) spp., we determined DD by correlation with broth...

Abstract Phytoplasmas are bacterial pathogens located in the plant’s phloem that responsible for several plant diseases and mainly transmitted by phloem-sucking insects. Apple proliferation (AP) is an economically important disease caused ‘ Candidatus Phytoplasma mali’ which two psyllid species. While Cacopsylla picta a confirmed vector different regions, efficiency of melanoneura varies between populations. This species considered main AP Northwestern Italy but plays minor role Northeastern...

ABSTRACT Hemipteran insects are well-known for their ancient associations with beneficial bacterial endosymbionts, particularly nutritional symbionts providing the host essential nutrients such as amino acids or vitamins lacking from host’s diet. Thereby, these primary endosymbionts enable exploitation of nutrient-poor food sources plant sap vertebrate blood. In turn, strictly host-associated lifestyle strongly impacts genome evolution resulting in small and degraded genomes. Over time, even...

We report here the draft genome sequence of Paenibacillus polymyxa strain CCI-25, which displays strong antifungal and antibacterial activities in vitro The encompasses nonribosomal peptide synthetases predicted to encode a tridecaptin, polymyxin, fusaricidin, an iturin-like synthetase, lantibiotic similar paenicidin A, as well type 1 polyketide synthase.