A5088632099- Neuroendocrine regulation and behavior
- Stress Responses and Cortisol
- Circadian rhythm and melatonin
- CRISPR and Genetic Engineering
- Yeasts and Rust Fungi Studies
- Adrenal Hormones and Disorders
- Biosensors and Analytical Detection
- Neurotransmitter Receptor Influence on Behavior
- Berry genetics and cultivation research
- Animal Genetics and Reproduction
- Plant Pathogens and Fungal Diseases
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
University of Wisconsin–Madison
2004-2025
Abstract Porcine biomedical models have emerged as valuable tools in research due to their physiological, anatomical, metabolic, immunological, and genetic similarities humans. As a result, they offer greater relevance for translational studies than rodent models. Moreover, compared nonhuman primates, porcine are more cost-effective, easier manipulate genetically, raise fewer ethical concerns. However, the conventional breeds of swine most commonly used rapid growth rates, which lead...
Behavioral inhibition (BI) is an adaptive defensive response to threat; however, children who display extreme BI as a stable trait are at risk for development of anxiety disorders and depression. The present study validates rodent model based on ethologically relevant predator exposure paradigm. We show that individual differences in rat trait-like from adolescence into adulthood. Using situ hybridization quantify expression the immediate early genes homer1a fos measures neuronal activation,...
Significance We successfully identified the duper allele as a null mutation of Cryptochrome 1 in Syrian hamsters. Here, we have shown use fast homozygosity mapping an effective approach to identify causal mutations mammals, despite lacking chromosomal genome information. In course this work, improved draft hamster and generated datasets necessary exploit hamsters modern genetic research model. The unique physiological features make them desirable model investigate human diseases, including...
Corticotropin-releasing factor (CRF) is a neurotransmitter and hormone believed to integrate responses stress. Evidence suggests central CRF systems are overactive in some individuals suffering from depression anxiety disorders. receptor antagonism blocks stress-induced endocrine, autonomic, behavioral effects animal models, studies have implicated the CRF2 anxiety-related behaviors. Greater understanding of regulation expression may facilitate mechanisms underlying anxiety. The present...
The COVID-19 pandemic necessitated sensitive, fast and inexpensive testing for the virus on university campuses across nation in 2020 prior to widespread availability of vaccines. Early efforts were limited by bottlenecks reagents, low throughput options slow return test results. In this paper we detail pipeline established at University Wisconsin-Madison rapid, sensitive surveillance SARS-CoV-2 highlight strengths platform that would allow it be applied other disease projects, variant or...
Behavioral inhibition (BI) is an adaptive defensive response to threat that has been evolutionarily conserved. Extreme BI in childhood a temperamental disposition for the development of psychopathology. Our goal was develop rodent model elicited by predator exposure. The ferret exposure test consists placing rat housing cage colony room 15 min and rating behavior BI. We show predator‐induced stable across when measured rats (n = 37) adolescence again adulthood (r 0.475, p < 0.01). reduced...
ABSTRACT Transgenic rodent models for human diseases have been widely used over the past 50 years and are a mainstay of many biomedical research programs. Oftentimes sequence transgenic segment DNA is carefully designed but incorporation this into host genome less well understood. Structural variation insertional mutagenesis may occur at insertion sites. Here, we present robust workflow including identification transgene locus via selective Illumina sequencing followed by Cas9-mediated...