A5046785835- Bioenergy crop production and management
- Biofuel production and bioconversion
- Plant nutrient uptake and metabolism
- Insect-Plant Interactions and Control
- Legume Nitrogen Fixing Symbiosis
- Plant Gene Expression Analysis
- Lignin and Wood Chemistry
- Ammonia Synthesis and Nitrogen Reduction
- Forest Biomass Utilization and Management
- Enzyme-mediated dye degradation
- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- Photosynthetic Processes and Mechanisms
- Plant biochemistry and biosynthesis
- Turfgrass Adaptation and Management
- Plant Virus Research Studies
- Agronomic Practices and Intercropping Systems
- Hemiptera Insect Studies
- Plant Parasitism and Resistance
- Insect Resistance and Genetics
- Nematode management and characterization studies
- Animal Genetics and Reproduction
- Plant Micronutrient Interactions and Effects
- Heme Oxygenase-1 and Carbon Monoxide
- Insect symbiosis and bacterial influences
University of Nebraska at Kearney
2010-2023
University of Nebraska–Lincoln
2008-2011
University of Tennessee at Knoxville
1993
The phenylpropanoid biosynthetic pathway that generates lignin subunits represents a significant target for altering the abundance and composition of lignin. global regulators metabolism may include MYB transcription factors, whose expression levels have been correlated with changes in secondary cell wall several other aromatic compounds, including anthocyanins flavonoids. While factors downregulation biosynthesis identified grass species, few linked to activation this C4 grasses, some which...
Aphids are, arguably, the single most damaging group of agricultural insect pests throughout world. Plant tolerance, which is a plant response to an pest, viewed as excellent management strategy. Developing testable hypotheses based on genome-wide and more focused methods will help in understanding molecular underpinnings tolerance aphid herbivory. As first step this process, we undertook transcript profiling with Affymetrix GeneChip Barley Genome arrays using RNA extracted from tissues...
The development of genomic resources for switchgrass ( Panicum virgatum L.), a perennial NAD + ‐malic enzyme type C 4 grass, is required to enable molecular breeding and biotechnological approaches improving its value as forage bioenergy crop. Expressed sequence tag (EST) sequencing one method that can quickly sample gene inventories produce data suitable marker or analysis tissue‐specific patterns expression. Toward this goal, three cDNA libraries from callus, crown, seedling tissues...
Aphid infestation of switchgrass (Panicum virgatum) has the potential to reduce yields and biomass quality. Although switchgrass-greenbug (Schizaphis graminum; GB) interactions have been studied at whole plant level, little information is available on defense responses molecular level. The global transcriptomic response cv Summer GB was monitored by RNA-Seq in infested control (uninfested) plants harvested 5, 10, 15 days after (DAI). Differentially expressed genes (DEGs) were analyzed...
Summary Few transcription factors have been identified in C 4 grasses that either positively or negatively regulate monolignol biosynthesis. Previously, the overexpression of SbMyb60 sorghum ( Sorghum bicolor ) has shown to induce biosynthesis, which leads elevated lignin deposition and altered cell wall composition. To determine how impacts other metabolic pathways, RNA ‐Seq metabolite profiling were performed on stalks leaves. 35S::SbMyb60 was associated with transcriptional activation...
Flavonoids are potent antioxidants that play a role in defense against pathogens, UV-radiation, and the detoxification of reactive oxygen species. Dihydroflavonol 4-reductase (DFR) flavanone (FNR) reduce dihydroflavonols flavanones, respectively, using NAD(P)H to produce flavan-(3)-4-(di)ols flavonoid biosynthesis. Anthocyanidin reductase (ANR) reduces anthocyanidins flavan-3-ols. In addition their sequences, 3D structures recombinant DFR, FNR ANR from sorghum switchgrass showed high level...
Two cDNAs representing different members (agNt84 and ag164) of a gene family encoding glycine- histidinerich proteins have been isolated from cDNA libraries Alnus glutinosa root nodules. Expression the corresponding genes could only be detected in With situ hybridization, expression nodules was found to occur young, infected cells prefixation zone (zone 2). The encoded contain putative signal peptides for targeting endomembrane system, sharing sequence similarity with plant glycinerich...
Abstract Switchgrass is a large, North American, perennial grass that being evaluated as potential energy crop. There need to assess genetic diversity in stored accessions and remaining native stands assist breeding conservation efforts. Marker development will also be necessary for linkage mapping. Toward this end, 32 switchgrass genic di‐, tri‐ tetranucleotide repeat microsatellites were identified from expressed sequence tags (ESTs). These used screen individuals two different named...
Background Switchgrass (Panicum virgatum L.) is being developed as a bioenergy crop for many temperate regions of the world. One way to increase biomass yields move southern adapted lowland cultivars more northern latitudes. However, southerly switchgrass germplasm can suffer significant winter kill in northerly climes. Materials and Methods Here, we have applied next-generation sequencing combination with biochemical analyses query metabolism crowns rhizomes obtained from two contrasting...
Aphid herbivory elicits plant defense-related networks that are influenced by host genetics. Plants of the upland switchgrass (Panicum virgatum) cultivar Summer can be a suitable for greenbug aphids (Schizaphis graminum; GB), and yellow sugarcane (Sipha flava, YSA), whereas lowland Kanlow exhibited multi-species resistance curtails aphid reproduction. However, stabilized hybrids (♀) x (♂) (SxK) with improved agronomics damaged both aphids. Here, hormone metabolite analyses, coupled RNA-Seq...
Nonsymbiotic hemoglobins (nsHbs) and leghemoglobins (Lbs) are plant proteins that can reversibly bind O(2) other ligands. The nsHbs hexacoordinate appear to modulate cellular concentrations of NO maintain energy levels under hypoxic conditions. Lbs pentacoordinate facilitate the diffusion symbiotic bacteroids within legume root nodules. Multiple lines evidence suggest all Hbs evolved from a common ancestor originated nsHbs. However, little is known about structural intermediates occurred...
Herbaceous perennial plants selected as potential biofuel feedstocks had been understudied at the genomic and functional levels. Recent investments, primarily by U.S. Department of Energy, have led to development a number molecular resources for bioenergy grasses, such partially annotated genome switchgrass (Panicum virgatum L.), some related diploid species. In its current version, contains 65,878 gene models arising from A B genomes this tetraploid grass. The availability these sequences...
Switchgrass (Panicum virgatum), a perennial, polyploid, C4 warm-season grass is among the foremost herbaceous species being advanced as source of biomass for biofuel end uses. At every growing season, aerial tissues senesce, and below-ground rhizomes become dormant. Future growth dependent on successful over-wintering rhizomes. Although importance rhizome health to overall year-upon-year plant productivity has been long recognized, there limited information seasonal changes occurring during...
Switchgrass (Panicum virgatum L) is perennial, C4 grass with great potential as a biofuel crop. An in-depth understanding of the mechanisms that control mineral uptake, distribution and remobilization will benefit sustainable production. Nutrients are mobilized from aerial portions to below-ground crowns rhizomes natural accompaniment above-ground senescence post seed-set. Mineral uptake dependent on transporters, however, little if any information available about specific transporters...
Oxidative enzymes are one of many key players in plant tolerance responses and defense signaling pathways. This study evaluated gene expression four buffalograss transcripts (two peroxidases, a catalase, GRAS (gibberellic acid insensitive [GAI], repressor GAI, scarecrow) total peroxidase activity response to western chinch bug (Blissus occiduus Barber) feeding susceptible resistant buffalograsses (Buchloë dactyloides (Nuttall) Engelmann). Basal levels all were consistently higher the when...
Abstract Background Buffalograss [ Buchloë dactyloides (Nutt.) Engel. syn. Bouteloua Columbus] is a United States native turfgrass species that requires less irrigation, fungicides and pesticides compared to more commonly used species. In areas where water limited, interest in this grass for lawns increasing. While several buffalograss cultivars have been developed through breeding, the timeframe new cultivar development long limited by lack of useful genetic resources. Two high throughput...