A5086430736- Plant Pathogens and Fungal Diseases
- CRISPR and Genetic Engineering
- Transgenic Plants and Applications
- Photosynthetic Processes and Mechanisms
- Plant Disease Resistance and Genetics
- Plant tissue culture and regeneration
- Genetically Modified Organisms Research
- Mitochondrial Function and Pathology
- Fungal and yeast genetics research
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- Fungal Biology and Applications
- Microbial Natural Products and Biosynthesis
- Mycorrhizal Fungi and Plant Interactions
- RNA regulation and disease
- Plant-Microbe Interactions and Immunity
- Plant Reproductive Biology
- Plant and fungal interactions
- Nematode management and characterization studies
- Plant Molecular Biology Research
- Entomopathogenic Microorganisms in Pest Control
- Plant Virus Research Studies
- Yeasts and Rust Fungi Studies
- Protist diversity and phylogeny
- Marine Sponges and Natural Products
Kiel University
2013-2024
Ruhr University Bochum
1990-2002
University of Florida
1991-1997
Institut für Zeitgeschichte München–Berlin
1997
Filamentous fungi are of great importance in ecology, agriculture, medicine, and biotechnology. Thus, it is not surprising that genomes for more than 100 filamentous have been sequenced, most them by Sanger sequencing. While next-generation sequencing techniques revolutionized genome resequencing, e.g. strain comparisons, genetic mapping, or transcriptome ChIP analyses, de novo assembly eukaryotic still presents significant hurdles, because their large size stretches repetitive sequences....
The vast repertoire of toxic fungal secondary metabolites has long been assumed to have an evolved protective role against fungivory. It still remains elusive, however, whether fungi contain these compounds as anti-predator adaptation. We demonstrate that loss in the soil mould Aspergillus nidulans causes, under attack fungivorous springtail Folsomia candida , a disadvantage fungus. Springtails exhibited distinct preference for feeding on mutant deleted LaeA, global regulator metabolites....
Fungi are a large group of eukaryotes found in nearly all ecosystems. More than 250 fungal genomes have already been sequenced, greatly improving our understanding evolution, physiology, and development. However, for the Pezizomycetes, an early-diverging lineage filamentous ascomycetes, there is so far only one genome available, namely that black truffle, Tuber melanosporum, mycorrhizal species with unusual subterranean fruiting bodies. To help close sequence gap among basal to allow...
RNA editing and cytoplasmic male sterility are two important phenomena in higher plant mitochondria. To determine whether correlations might exist between the two, different tissues of Sorghum bicolor was compared employing reverse transcription–PCR subsequent sequence analysis. In etiolated shoots, transcripts mitochondrial atp6 , atp9 nad3 nad4 rps12 genes identical among fertile or sterile plants. We then established a protocol for isolation from anthers pollen to include these studies....
AbstractElements of the hAT transposon family, such as maize activator (Ac), have been discovered in a large number eukaryotic species. This type class II transposon, present both plants and animals, has not previously detected any fungal organism. However, using differential screening method to detect repetitive DNA, we identified hyphomycete Tolypocladium inflatum. The which named restless, is 4,097 bp long, carries 20-bp inverted repeats an 8-bp target site duplication, encodes long open...
Plants, bacteria and some fungi are known to produce indole-3-acetic acid (IAA) by employing various pathways. Among these pathways, the indole-3-pyruvic (IPA) pathway is best studied in green plants plant-associated beneficial microbes. While IAA production circuitry has been for decades, little regarding biosynthesis fungal species. Here, we present first data IAA-producing genes associated a non-pathogenic fungus, Neurospora crassa. For this purpose, used computational approach determine...
Abstract Marine Fungi are potent secondary metabolite producers. However, limited genetic information available their biosynthetic gene clusters (BGCs) and biotechnological applications. To overcome this lack of information, herein, we used next-generation sequencing methods for genome two marine fungi, isolated from the German Wadden Sea, namely Calcarisporium sp. KF525 Pestalotiopsis KF079. The assembled size isolate is about 36.8 Mb with 60 BGCs, while s p. KF079 has a 47.5 harboring 67...
The marine-derived Scopulariopsis brevicaulis strain LF580 produces scopularides A and B, which have anticancerous properties. We carried out genome sequencing using three next-generation DNA methods. De novo hybrid assembly yielded 621 scaffolds with a total size of 32.2 Mb 16298 putative gene models. identified large non-ribosomal peptide synthetase (nrps1) supporting pks2 in the same biosynthetic cluster. This cluster genes within are functionally active as confirmed by RNA-Seq....
Scopularide A is a promising potent anticancer lipopeptide isolated from marine derived Scopulariopsis brevicaulis strain. The compound consists of reduced carbon chain (3-hydroxy-methyldecanoyl) attached to five amino acids (glycine, l-valine, d-leucine, l-alanine, and l-phenylalanine). Using the newly sequenced S. genome we were able identify putative biosynthetic gene cluster using genetic information structurally related emericellamide Aspergillus nidulans W493-B Fusarium...
Flowering is a vital developmental shift in plants from vegetative to reproductive phase. The timing of this regulated by various linked genetic pathways including environmental cues and internal regulation. Here we report role for an Arabidopsis gene, AT1G15480, which encodes P-class pentatricopeptide repeat (PPR) protein, affecting flowering time. We show that AT1G15480 localized mitochondria. An T-DNA insertion line exhibits early-flowering phenotype, quite rare phenotype among PPR...
Red yeasts of the genus Rhodotorula are great interest to biotechnological industry due their ability produce valuable natural products, such as lipids and carotenoids with potential applications surfactants, food additives, pharmaceuticals. Herein, we explored biosynthetic R. mucilaginosa 50-3-19/20B collected from Mid-Atlantic Ridge using modern genomics untargeted metabolomics tools. exhibited anticancer activity when grown on PDA medium, while antimicrobial was observed cultured WSP-30...