A5033868176- Redox biology and oxidative stress
- Metal-Catalyzed Oxygenation Mechanisms
- Photosynthetic Processes and Mechanisms
- Glutathione Transferases and Polymorphisms
- Vanadium and Halogenation Chemistry
- Plant Gene Expression Analysis
- Sulfur Compounds in Biology
- Plant Stress Responses and Tolerance
Centre National de la Recherche Scientifique
2006-2012
Institut des Sciences des Plantes de Paris Saclay
2012
Université Paris-Sud
2006-2008
Glutaredoxins (GRXs) are small ubiquitous disulfide oxidoreductases known to use GSH as electron donor. In photosynthetic organisms, little is about the biochemical properties of GRXs despite existence approximately 30 different isoforms in higher plants. We report here characterization Chlamydomonas GRX1 and GRX3, major cytosolic chloroplastic isoforms, respectively. classified on basis amino acid sequence active site. a typical CPYC-type GRX, which reduced by exhibits reductase,...
ABSTRACT Chloroplast thioredoxin f (Trx ) is an important regulator of primary metabolic enzymes. However, genetic evidence for its physiological importance largely lacking. To test the functional significance Trx in vivo , Arabidopsis mutants with insertions trx 1 gene were studied, showing a drastic decrease leaf content. Knockout led to strong attenuation reductive light activation ADP‐glucose pyrophosphorylase (AGPase), key enzyme starch synthesis, leaves during day and isolated...
Methionine sulfoxide reductases (MSRs) A and B reduce methionine (MetSO) S ‐ R ‐diastereomers, respectively, back to Met using electrons generally supplied by thioredoxin. The physiological reductants for MSRBs remain unknown in plants, which display a remarkable variety of thioredoxins (Trxs) glutaredoxins (Grxs). Using recombinant proteins, we show that Arabidopsis plastidial MSRB1 MSRB2, differ regarding the number presumed redox‐active cysteines, possess specific reductants. Most...
In plants, thioredoxins (TRX) constitute a large protein disulphide oxidoreductase family comprising 10 plastidial members in Arabidopsis thaliana and subdivided five types. The f- m-types regulate enzymes involved mainly carbon metabolism whereas the x, y, z types have an antioxidant function. reduction of TRXm f chloroplasts is performed light by ferredoxin:thioredoxin reductase (FTR) that uses photosynthetically reduced ferredoxin (Fd) as reductant. system TRXx, has never been...